Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41893-019-0302-6 |
Enzyme morphinan N-demethylase for more sustainable opiate processing | |
Augustin, M. M.1; Augustin, J. M.1,3; Brock, J. R.1,2; Kutchan, T. M.1 | |
2019-06-01 | |
发表期刊 | NATURE SUSTAINABILITY |
ISSN | 2398-9629 |
出版年 | 2019 |
卷号 | 2期号:6页码:465-474 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Naturally occurring opiates, such as morphine and thebaine, are produced in poppy species. Thebaine is converted into painkillers and opiate addiction treatments, the latter requiring a chemical reaction called N-demethylation. Current opiate N-demethylation utilizes noxious reagents, resulting in copious amounts of harmful waste. One way to make opiate production more sustainable is to use enzymes rather than chemicals. Microorganisms provide a rich source of enzymes useful for metabolizing unique compounds in their environment. Therefore, an opium-processing waste stream was probed to identify an organism capable of catalysing opiate N-demethylation. A sludge sample was grown on minimal medium containing thebaine as the sole carbon source, to identify a biocatalyst. This led to the discovery of Thebainfresser, a Methylobacterium that metabolizes opiates by removing the N-methyl group. N-demethylation was induced following growth in minimal medium, a characteristic which led to discovery of the underlying gene MND (morphinan N-demethylase). The enzyme MND was found to be robust and versatile, N-demethylating structurally diverse substrates at varying temperatures and pH levels. In addition, MND tolerated selected organic solvents and maintained activity when immobilized. These properties make it an attractive candidate for further development for pharmaceutical manufacture. |
领域 | 资源环境 |
收录类别 | SSCI |
WOS记录号 | WOS:000471032700010 |
WOS关键词 | METHYLOBACTERIUM-EXTORQUENS AM1 ; GREEN CHEMISTRY ; ALKALOIDS ; IDENTIFICATION ; CHLOROMETHANICUM ; TRANSFORMATION ; BIOSYNTHESIS ; OXIDATION ; THEBAINE ; BACTERIA |
WOS类目 | Green & Sustainable Science & Technology ; Environmental Sciences ; Environmental Studies |
WOS研究方向 | Science & Technology - Other Topics ; Environmental Sciences & Ecology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/289821 |
专题 | 资源环境科学 |
作者单位 | 1.Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA; 2.Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA; 3.Elemental Enzymes, St Louis, MO USA |
推荐引用方式 GB/T 7714 | Augustin, M. M.,Augustin, J. M.,Brock, J. R.,et al. Enzyme morphinan N-demethylase for more sustainable opiate processing[J]. NATURE SUSTAINABILITY,2019,2(6):465-474. |
APA | Augustin, M. M.,Augustin, J. M.,Brock, J. R.,&Kutchan, T. M..(2019).Enzyme morphinan N-demethylase for more sustainable opiate processing.NATURE SUSTAINABILITY,2(6),465-474. |
MLA | Augustin, M. M.,et al."Enzyme morphinan N-demethylase for more sustainable opiate processing".NATURE SUSTAINABILITY 2.6(2019):465-474. |
条目包含的文件 | 条目无相关文件。 |
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