A single-cell RNA-seq atlas of <em>Schistosoma mansoni</em> identifies a key regulator of blood feeding

doi:10.1126/science.abb7709

GSTDTAP > 气候变化

DOI	10.1126/science.abb7709
	*A single-cell RNA-seq atlas of Schistosoma mansoni* identifies a key regulator of blood feeding**
	George Wendt; Lu Zhao; Rui Chen; Chenxi Liu; Anthony J. O’Donoghue; Conor R. Caffrey; Michael L. Reese; James J. Collins
	2020-09-25
发表期刊	Science
出版年	2020
英文摘要	Schistosomiasis is caused by a parasitic flatworm about which little is known. Therefore, options to combat human disease caused by schistosome infection are limited. To aid in our quest to develop treatments, two studies undertook molecular investigations of the parasite Schistosoma mansoni . By generating a single-cell atlas, Wendt et al. identified the developmental trajectory of the flatworm, including the blood-feeding gut required for its survival in the host. From these data, they found a gene required for gut development that, when knocked out through RNA interference, confers reduced pathology in infected mice. Wang et al. performed a large-scale RNA interference survey of S. mansoni and identified an essential pair of protein kinases that can be targeted by approved pharmacological intervention (see the Perspective by Anderson and Duraisingh). These molecular investigations add to our understanding of the schistosome parasite and provide biological information that may help to combat this neglected tropical disease. Science , this issue p. [1644][1], p. [1649][2]; see also p. [1562][3] Schistosomiasis is a neglected tropical disease that infects 240 million people. With no vaccines and only one drug available, new therapeutic targets are needed. The causative agents, schistosomes, are intravascular flatworm parasites that feed on blood and lay eggs, resulting in pathology. The function of the parasite’s various tissues in successful parasitism are poorly understood, hindering identification of therapeutic targets. Using single-cell RNA sequencing (RNA-seq), we characterize 43,642 cells from the adult schistosome and identify 68 distinct cell populations, including specialized stem cells that maintain the parasite’s blood-digesting gut. These stem cells express the gene hnf4 , which is required for gut maintenance, blood feeding, and pathology in vivo. Together, these data provide molecular insights into the organ systems of this important pathogen and identify potential therapeutic targets. [1]: /lookup/doi/10.1126/science.abb7709 [2]: /lookup/doi/10.1126/science.abb7699 [3]: /lookup/doi/10.1126/science.abe0710
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/296528
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	George Wendt,Lu Zhao,Rui Chen,et al. A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding[J]. Science,2020.
APA	George Wendt.,Lu Zhao.,Rui Chen.,Chenxi Liu.,Anthony J. O’Donoghue.,...&James J. Collins.(2020).A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding.Science.
MLA	George Wendt,et al."A single-cell RNA-seq atlas of Schistosoma mansoni identifies a key regulator of blood feeding".Science (2020).