The mouse <em>Sry</em> locus harbors a cryptic exon that is essential for male sex determination

doi:10.1126/science.abb6430

GSTDTAP > 气候变化

DOI	10.1126/science.abb6430
	*The mouse Sry* locus harbors a cryptic exon that is essential for male sex determination**
	Shingo Miyawaki; Shunsuke Kuroki; Ryo Maeda; Naoki Okashita; Peter Koopman; Makoto Tachibana
	2020-10-02
发表期刊	Science
出版年	2020
英文摘要	For several decades, it has been believed that the mammalian sex-determining gene Sry contains a single exon. Miyawaki et al. have now identified a cryptic second exon of mouse Sry . Loss- and gain-of-function analyses revealed that the two-exon SRY (SRY-T), not the canonical single exon–encoded SRY (SRY-S), is the bona fide testis-determining factor. Sry exon2 is composed of retrotransposon-derived sequences. The SRY-S carboxyl terminus contains a degradation sequence (degron), whereas the SRY-T carboxyl terminus encoded in the Sry exon2 is degron free, thereby conferring protein stability on SRY-T. Science , this issue p. [121][1] The mammalian sex-determining gene Sry induces male development. Since its discovery 30 years ago, Sry has been believed to be a single-exon gene. Here, we identified a cryptic second exon of mouse Sry and a corresponding two-exon type Sry ( Sry-T ) transcript. XY mice lacking Sry-T were sex-reversed, and ectopic expression of Sry-T in XX mice induced male development. Sry-T messenger RNA is expressed similarly to that of canonical single-exon type Sry ( Sry-S ), but SRY-T protein is expressed predominantly because of the absence of a degron in the C terminus of SRY-S. Sry exon2 appears to have evolved recently in mice through acquisition of a retrotransposon-derived coding sequence to replace the degron. Our findings suggest that in nature, SRY-T, not SRY-S, is the bona fide testis-determining factor. [1]: /lookup/doi/10.1126/science.abb6430
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/298063
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Shingo Miyawaki,Shunsuke Kuroki,Ryo Maeda,et al. The mouse Sry locus harbors a cryptic exon that is essential for male sex determination[J]. Science,2020.
APA	Shingo Miyawaki,Shunsuke Kuroki,Ryo Maeda,Naoki Okashita,Peter Koopman,&Makoto Tachibana.(2020).The mouse Sry locus harbors a cryptic exon that is essential for male sex determination.Science.
MLA	Shingo Miyawaki,et al."The mouse Sry locus harbors a cryptic exon that is essential for male sex determination".Science (2020).