In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

doi:10.1126/science.abd5223

GSTDTAP > 气候变化

DOI	10.1126/science.abd5223
	In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges
	Beata Turoňová; Mateusz Sikora; Christoph Schürmann; Wim J. H. Hagen; Sonja Welsch; Florian E. C. Blanc; Sören von Bülow; Michael Gecht; Katrin Bagola; Cindy Hörner; Ger van Zandbergen; Jonathan Landry; Nayara Trevisan Doimo de Azevedo; Shyamal Mosalaganti; Andre Schwarz; Roberto Covino; Michael D. Mühlebach; Gerhard Hummer; Jacomine Krijnse Locker; Martin Beck
	2020-10-09
发表期刊	Science
出版年	2020
英文摘要	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein enables viral entry into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor and is a major target for neutralizing antibodies. About 20 to 40 spikes decorate the surface of virions. Turoňová et al. now show that the spike is flexibly connected to the viral surface by three hinges that are well protected by glycosylation sites. The flexibility imparted by these hinges may explain how multiple spikes act in concert to engage onto the flat surface of a host cell. Science , this issue p. [203][1] The spike protein (S) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is required for cell entry and is the primary focus for vaccine development. In this study, we combined cryo–electron tomography, subtomogram averaging, and molecular dynamics simulations to structurally analyze S in situ. Compared with the recombinant S, the viral S was more heavily glycosylated and occurred mostly in the closed prefusion conformation. We show that the stalk domain of S contains three hinges, giving the head unexpected orientational freedom. We propose that the hinges allow S to scan the host cell surface, shielded from antibodies by an extensive glycan coat. The structure of native S contributes to our understanding of SARS-CoV-2 infection and potentially to the development of safe vaccines. [1]: /lookup/doi/10.1126/science.abd5223
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/298096
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Beata Turoňová,Mateusz Sikora,Christoph Schürmann,et al. In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges[J]. Science,2020.
APA	Beata Turoňová.,Mateusz Sikora.,Christoph Schürmann.,Wim J. H. Hagen.,Sonja Welsch.,...&Martin Beck.(2020).In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges.Science.
MLA	Beata Turoňová,et al."In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges".Science (2020).