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DOI | 10.1289/EHP7699 |
DNA Methylation Patterns in T Cells of Naïve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand | |
Catherine G. Burke; Jason R. Myers; Christina M. Post; Lisbeth A. Boulé; B. Paige Lawrence | |
2021-01-15 | |
发表期刊 | Environmental Health Perspectives
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出版年 | 2021 |
英文摘要 | AbstractBackground:Early life environmental exposures can have lasting effects on the function of the immune system and contribute to disease later in life. Epidemiological studies have linked early life exposure to xenobiotics that bind the aryl hydrocarbon receptor (AhR) with dysregulated immune responses later in life. Among the immune cells influenced by developmental activation of the AhR are T cells. Yet, the underlying affected cellular pathways via which activating the AhR early in life causes the responses of T cells to remain affected into adulthood remain unclear. Objective:Our goal was to identify cellular mechanisms that drive impaired T-cell responses later in life following maternal exposure to an exogenous AhR ligand. Methods:C57BL/6 mice were vertically exposed to the prototype AhR ligand, 2,3,7,8-tetrachlorodibenzo--dioxin (TCDD), throughout gestation and early postnatal life. The transcriptome and DNA methylation patterns were evaluated in T cells isolated from naïve and influenza A virus (IAV)-infected adult mice that were developmentally exposed to TCDD or vehicle control. We then assessed the influence of DNA methylation-altering drug therapies on the response of T cells from developmentally exposed mice to infection. Results:Gene and protein expression showed that developmental AhR activation reduced T-cell expansion and effector functions during IAV infection later in life. Furthermore, whole-genome bisulfite sequencing analyses revealed that developmental AhR activation durably programed DNA methylation patterns across the T-cell genome. Treatment of developmentally exposed offspring with DNA methylation-altering drugs alleviated some, but not all, of the impaired T-cell responses. Discussion:Taken together, these results indicate that skewed DNA methylation is one of the mechanisms by which early life exposures can durably change the function of T cells in mice. Furthermore, treatment with DNA methylation-altering drugs after the exposure restored some aspects of T-cell functional responsiveness. https://doi.org/10.1289/EHP7699 |
领域 | 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/311286 |
专题 | 资源环境科学 |
推荐引用方式 GB/T 7714 | Catherine G. Burke,Jason R. Myers,Christina M. Post,et al. DNA Methylation Patterns in T Cells of Naïve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand[J]. Environmental Health Perspectives,2021. |
APA | Catherine G. Burke,Jason R. Myers,Christina M. Post,Lisbeth A. Boulé,&B. Paige Lawrence.(2021).DNA Methylation Patterns in T Cells of Naïve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand.Environmental Health Perspectives. |
MLA | Catherine G. Burke,et al."DNA Methylation Patterns in T Cells of Naïve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand".Environmental Health Perspectives (2021). |
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