Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1002/fee.1811 |
Early detection of invasive exotic insect infestations using eDNA from crop surfaces | |
Valentin, Rafael E.1; Fonseca, Dina M.1,2; Nielsen, Anne L.2; Leskey, Tracy C.3; Lockwood, Julie L.1 | |
2018-06-01 | |
发表期刊 | FRONTIERS IN ECOLOGY AND THE ENVIRONMENT |
ISSN | 1540-9295 |
EISSN | 1540-9309 |
出版年 | 2018 |
卷号 | 16期号:5页码:265-270 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The number of exotic species invasions has increased over recent decades, as have the ecological harm and economic burdens they impose. Rapid-response eradication of nascent exotic populations is a viable approach to minimizing damage, but implementation is limited by the difficulty of detecting such species during the early stages of infestation due to their small numbers. The use of environmental DNA (eDNA) has helped address this issue in aquatic ecosystems, but to the best of our knowledge has not been trialed for surveillance of exotic species in terrestrial systems. Using a high-resolution, real-time (quantitative) polymerase chain reaction assay, we developed a highly efficient protocol to survey agricultural fields for the invasive non-native brown marmorated stink bug (BMSB; Halyomorpha halys). We compared results using eDNA to those for conventional monitoring traps and documented substantially higher sensitivity and detection effectiveness. Our methodology is transferable to situations in which the DNA of terrestrial target species can be accumulated into a single substrate, suggesting that eDNA-based approaches could transform our ability to detect exotic insects in non-aquatic settings. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000434222100016 |
WOS关键词 | HALYS HEMIPTERA-PENTATOMIDAE ; ENVIRONMENTAL DNA ; BIOLOGICAL INVASIONS ; ABUNDANCE ; ECOLOGY ; IMPACT ; STAL |
WOS类目 | Ecology ; Environmental Sciences |
WOS研究方向 | Environmental Sciences & Ecology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/31841 |
专题 | 资源环境科学 |
作者单位 | 1.Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ USA; 2.Rutgers State Univ, Dept Entomol, New Brunswick, NJ USA; 3.ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA |
推荐引用方式 GB/T 7714 | Valentin, Rafael E.,Fonseca, Dina M.,Nielsen, Anne L.,et al. Early detection of invasive exotic insect infestations using eDNA from crop surfaces[J]. FRONTIERS IN ECOLOGY AND THE ENVIRONMENT,2018,16(5):265-270. |
APA | Valentin, Rafael E.,Fonseca, Dina M.,Nielsen, Anne L.,Leskey, Tracy C.,&Lockwood, Julie L..(2018).Early detection of invasive exotic insect infestations using eDNA from crop surfaces.FRONTIERS IN ECOLOGY AND THE ENVIRONMENT,16(5),265-270. |
MLA | Valentin, Rafael E.,et al."Early detection of invasive exotic insect infestations using eDNA from crop surfaces".FRONTIERS IN ECOLOGY AND THE ENVIRONMENT 16.5(2018):265-270. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论