<em>Debaryomyces</em> is enriched in Crohn’s disease intestinal tissue and impairs healing in mice

doi:10.1126/science.abd0919

GSTDTAP > 气候变化

DOI	10.1126/science.abd0919
	Debaryomyces is enriched in Crohn’s disease intestinal tissue and impairs healing in mice
	Umang Jain; Aaron M. Ver Heul; Shanshan Xiong; Martin H. Gregory; Elora G. Demers; Justin T. Kern; Chin-Wen Lai; Brian D. Muegge; Derek A. G. Barisas; J. Steven Leal-Ekman; Parakkal Deepak; Matthew A. Ciorba; Ta-Chiang Liu; Deborah A. Hogan; Philip Debbas; Jonathan Braun; Dermot P. B. McGovern; David M. Underhill; Thaddeus S. Stappenbeck
	2021-03-12
发表期刊	Science
出版年	2021
英文摘要	The gut microbiota includes not only prokaryotes, viruses, protists, and occasionally helminths, but also fungi. The role that fungi play in this symbiosis has long been overlooked. While investigating alterations to the gut microbiota in mice with mucosal damage and human subjects with Crohn's disease, Jain et al. discovered the fungus Debaryomyces hansenii localized to wounds in inflamed mucosal tissue (see the Perspective by Chiaro and Round). Impaired healing was associated with antibiotic treatment, overgrowth of the fungus, and subsequent induction of a type I interferon–CCL5 axis by macrophages. The fungus was observed within macrophages. Such persistent injury stimulus is a hallmark of inflammatory bowel diseases, including Crohn's disease and ulcerative colitis. It is not known whether this salt-tolerant fungus is a natural symbiont, but it is used in the food industry for surface ripening of cheese and meat products. Science , this issue p. [1154][1]; see also p. [1102][2] Alterations of the mycobiota composition associated with Crohn’s disease (CD) are challenging to link to defining elements of pathophysiology, such as poor injury repair. Using culture-dependent and -independent methods, we discovered that Debaryomyces hansenii preferentially localized to and was abundant within incompletely healed intestinal wounds of mice and inflamed mucosal tissues of CD human subjects. D. hansenii cultures from injured mice and inflamed CD tissues impaired colonic healing when introduced into injured conventionally raised or gnotobiotic mice. We reisolated D. hansenii from injured areas of these mice, fulfilling Koch’s postulates. Mechanistically, D. hansenii impaired mucosal healing through the myeloid cell–specific type 1 interferon–CCL5 axis. Taken together, we have identified a fungus that inhabits inflamed CD tissue and can lead to dysregulated mucosal healing. [1]: /lookup/doi/10.1126/science.abd0919 [2]: /lookup/doi/10.1126/science.abg6017
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/318696
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Umang Jain,Aaron M. Ver Heul,Shanshan Xiong,等. Debaryomyces is enriched in Crohn’s disease intestinal tissue and impairs healing in mice[J]. Science,2021.
APA	Umang Jain.,Aaron M. Ver Heul.,Shanshan Xiong.,Martin H. Gregory.,Elora G. Demers.,...&Thaddeus S. Stappenbeck.(2021).Debaryomyces is enriched in Crohn’s disease intestinal tissue and impairs healing in mice.Science.
MLA	Umang Jain,et al."Debaryomyces is enriched in Crohn’s disease intestinal tissue and impairs healing in mice".Science (2021).