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DOI | 10.1002/2017JD026797 |
Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography | |
Stagno, J. R.1; 39;Amare, A. R.2 | |
2017-01-12 | |
发表期刊 | NATURE |
ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 541期号:7636页码:242-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Germany |
英文摘要 | Riboswitches are structural RNA elements that are generally located in the 5' untranslated region of messenger RNA. During regulation of gene expression, ligand binding to the aptamer domain of a riboswitch triggers a signal to the downstream expression platform(1-3). A complete understanding of the structural basis of this mechanism requires the ability to study structural changes over time(4). Here we use femtosecond X-ray free electron laser (XFEL) pulses(5,6) to obtain structural measurements from crystals so small that diffusion of a ligand can be timed to initiate a reaction before diffraction. We demonstrate this approach by determining four structures of the adenine riboswitch aptamer domain during the course of a reaction, involving two unbound apo structures, one ligand-bound intermediate, and the final ligand-bound conformation. These structures support a reaction mechanism model with at least four states and illustrate the structural basis of signal transmission. The three-way junction and the P1 switch helix of the two apo conformers are notably different from those in the ligand-bound conformation. Our time-resolved crystallographic measurements with a 10-second delay captured the structure of an intermediate with changes in the binding pocket that accommodate the ligand. With at least a 10-minute delay, the RNA molecules were fully converted to the ligand-bound state, in which the substantial conformational changes resulted in conversion of the space group. Such notable changes in crystallo highlight the important opportunities that micro-and nanocrystals may offer in these and similar time-resolved diffraction studies. Together, these results demonstrate the potential of 'mix-and-inject' time-resolved serial crystallography to study biochemically important interactions between biomacromolecules and ligands, including those that involve large conformational changes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000396125500046 |
WOS关键词 | X-RAY-DIFFRACTION ; PHOTOACTIVE YELLOW PROTEIN ; STANDARD ATOMIC VOLUMES ; FEMTOSECOND CRYSTALLOGRAPHY ; BINDING RIBOSWITCHES ; MOLECULAR-STRUCTURE ; ADENINE RIBOSWITCH ; GENETIC-CONTROL ; SCATTERING DATA ; LIGAND |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/32181 |
专题 | 气候变化 |
作者单位 | 1.NCI, Prot Nucle Acid Interact Sect, Struct Biophys Lab, Ctr Canc Res, Frederick, MD 21702 USA; 2.Arizona State Univ, Dept Biochem, Tempe, AZ 85287 USA; 3.Arizona State Univ, Biodesign Inst, Ctr Appl Struct Discovery, Tempe, AZ 85287 USA; 4.Johns Hopkins Univ, Dept Biophys, Baltimore, MD 21218 USA; 5.NCI, Small Angle Xray Scattering Core Facil, Ctr Canc Res, Frederick, MD 21702 USA; 6.Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA; 7.Deutsch Elektronen Synchrotron DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany; 8.Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22607 Hamburg, Germany; 9.Hauptmann Woodward Med Res Inst, Buffalo, NY 14203 USA; 10.NIH, Ctr Informat Technol, Bldg 10, Bethesda, MD 20892 USA; 11.NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA; 12.NHLBI, Lab RNA Biophys & Cellular Physiol, NIH, Bldg 10, Bethesda, MD 20892 USA; 13.Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA; 14.SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA; 15.Argonne Natl Lab, Adv Photon Source, Struct Biol Ctr, Biosci Div, Argonne, IL 60439 USA; 16.Argonne Natl Lab, Adv Photon Source, Xray Sci Div, Argonne, IL 60439 USA; 17.NCI, Macromol Crystallog Lab, Ctr Canc Res, Frederick, MD 21702 USA |
推荐引用方式 GB/T 7714 | Stagno, J. R.,39;Amare, A. R.. Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography[J]. NATURE,2017,541(7636):242-+. |
APA | Stagno, J. R.,&39;Amare, A. R..(2017).Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography.NATURE,541(7636),242-+. |
MLA | Stagno, J. R.,et al."Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography".NATURE 541.7636(2017):242-+. |
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