A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes

doi:10.1126/science.abe6494

GSTDTAP > 气候变化

DOI	10.1126/science.abe6494
	A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes
	Dona Sleiman; Pierre Simon Garcia; Marion Lagune; Jerome Loc’h; Ahmed Haouz; Najwa Taib; Pascal Röthlisberger; Simonetta Gribaldo; Philippe Marlière; Pierre Alexandre Kaminski
	2021-04-30
发表期刊	Science
出版年	2021
英文摘要	Four nucleobases. adenine (A), cytosine (C), guanine (G), and thymine (T), are usually thought to be invariable in DNA. In bacterial viruses, however, each of the DNA bases have variations that help them to escape degradation by bacterial restriction enzymes. In the genome of cyanophage S-2L, A is completely replaced by diaminopurine (Z), which forms three hydrogen bonds with T and thus creates non–Watson-Crick base pairing in the DNA of this virus (see the Perspective by Grome and Isaacs). Zhou et al. and Sleiman et al. determined the biochemical pathway that produces Z, which revealed more Z genomes in viruses hosted in bacteria distributed widely in the environment and phylogeny. Pezo et al. identified a DNA polymerase that incorporates Z into DNA while rejecting A. These findings enrich our understanding of biodiversity and expand the genetic palette for synthetic biology. Science , this issue p. [512][1], [516][2], [520][3]; see also p. [460][4] Cells have two purine pathways that synthesize adenine and guanine ribonucleotides from phosphoribose via inosylate. A chemical hybrid between adenine and guanine, 2-aminoadenine (Z), replaces adenine in the DNA of the cyanobacterial virus S-2L. We show that S-2L and Vibrio phage PhiVC8 encode a third purine pathway catalyzed by PurZ, a distant paralog of succinoadenylate synthase (PurA), the enzyme condensing aspartate and inosylate in the adenine pathway. PurZ condenses aspartate with deoxyguanylate into dSMP (N6-succino-2-amino-2′-deoxyadenylate), which undergoes defumarylation and phosphorylation to give dZTP (2-amino-2′-deoxyadenosine-5′-triphosphate), a substrate for the phage DNA polymerase. Crystallography and phylogenetics analyses indicate a close relationship between phage PurZ and archaeal PurA enzymes. Our work elucidates the biocatalytic innovation that remodeled a DNA building block beyond canonical molecular biology. [1]: /lookup/doi/10.1126/science.abe4882 [2]: /lookup/doi/10.1126/science.abe6494 [3]: /lookup/doi/10.1126/science.abe6542 [4]: /lookup/doi/10.1126/science.abh3571
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/325041
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Dona Sleiman,Pierre Simon Garcia,Marion Lagune,et al. A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes[J]. Science,2021.
APA	Dona Sleiman.,Pierre Simon Garcia.,Marion Lagune.,Jerome Loc’h.,Ahmed Haouz.,...&Pierre Alexandre Kaminski.(2021).A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes.Science.
MLA	Dona Sleiman,et al."A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes".Science (2021).