Sense codon reassignment enables viral resistance and encoded polymer synthesis

doi:10.1126/science.abg3029

GSTDTAP > 气候变化

DOI	10.1126/science.abg3029
	Sense codon reassignment enables viral resistance and encoded polymer synthesis
	Wesley E. Robertson; Louise F. H. Funke; Daniel de la Torre; Julius Fredens; Thomas S. Elliott; Martin Spinck; Yonka Christova; Daniele Cervettini; Franz L. Böge; Kim C. Liu; Salvador Buse; Sarah Maslen; George P. C. Salmond; Jason W. Chin
	2021-06-04
发表期刊	Science
出版年	2021
英文摘要	Biological systems read all 64 triplet codons in DNA to encode the synthesis of proteins composed of 20 canonical amino acids. Robertson et al. created cells that do not read several codons and showed that this confers complete resistance to viruses, which normally rely on the host cell's ability to read all the codons in the viral genome to reproduce (see the Perspective by Jewel and Chatterjee). The authors reassigned each codon to several noncanonical amino acids (ncAAs). This advance enables the efficient synthesis of proteins containing three distinct ncAAs and the encoded synthesis of entirely noncanonical polymers and macrocycles. Science , abg3029, this issue p. [1057][1]; see also abi9892, p. [1040][2] It is widely hypothesized that removing cellular transfer RNAs (tRNAs)—making their cognate codons unreadable—might create a genetic firewall to viral infection and enable sense codon reassignment. However, it has been impossible to test these hypotheses. In this work, following synonymous codon compression and laboratory evolution in Escherichia coli , we deleted the tRNAs and release factor 1, which normally decode two sense codons and a stop codon; the resulting cells could not read the canonical genetic code and were completely resistant to a cocktail of viruses. We reassigned these codons to enable the efficient synthesis of proteins containing three distinct noncanonical amino acids. Notably, we demonstrate the facile reprogramming of our cells for the encoded translation of diverse noncanonical heteropolymers and macrocycles. [1]: /lookup/doi/10.1126/science.abg3029 [2]: /lookup/doi/10.1126/science.abi9892
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/329868
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Wesley E. Robertson,Louise F. H. Funke,Daniel de la Torre,et al. Sense codon reassignment enables viral resistance and encoded polymer synthesis[J]. Science,2021.
APA	Wesley E. Robertson.,Louise F. H. Funke.,Daniel de la Torre.,Julius Fredens.,Thomas S. Elliott.,...&Jason W. Chin.(2021).Sense codon reassignment enables viral resistance and encoded polymer synthesis.Science.
MLA	Wesley E. Robertson,et al."Sense codon reassignment enables viral resistance and encoded polymer synthesis".Science (2021).