Clonal analysis of immunodominance and cross-reactivity of the CD4 T cell response to SARS-CoV-2

doi:10.1126/science.abg8985

GSTDTAP > 气候变化

DOI	10.1126/science.abg8985
	Clonal analysis of immunodominance and cross-reactivity of the CD4 T cell response to SARS-CoV-2
	Jun Siong Low; Daniela Vaqueirinho; Federico Mele; Mathilde Foglierini; Josipa Jerak; Michela Perotti; David Jarrossay; Sandra Jovic; Laurent Perez; Rosalia Cacciatore; Tatiana Terrot; Alessandra Franzetti Pellanda; Maira Biggiogero; Christian Garzoni; Paolo Ferrari; Alessandro Ceschi; Antonio Lanzavecchia; Federica Sallusto; Antonino Cassotta
	2021-06-18
发表期刊	Science
出版年	2021
英文摘要	A better understanding of CD4+ T cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial to the design of effective next-generation vaccines. Low et al. defined and estimated the CD4+ T cell repertoire of convalescent COVID-19 patients. After sorting various CD4+ T cell subsets, they generated numerous T cell clones that reacted to the SARS-CoV-2 spike protein. In around a third of all clones and almost all individuals, the T cells recognized a small conserved immunodominant region within the spike protein receptor-binding domain (RBD). The researchers isolated T cell clones that broadly reacted to the spike protein of other coronaviruses, providing evidence for the recall of preexisting cross-reactive memory T cells after SARS-CoV-2 infection. Science , abg8985, this issue p. [1336][1] The identification of CD4+ T cell epitopes is instrumental for the design of subunit vaccines for broad protection against coronaviruses. Here, we demonstrate in COVID-19–recovered individuals a robust CD4+ T cell response to naturally processed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein and nucleoprotein (N), including effector, helper, and memory T cells. By characterizing 2943 S-reactive T cell clones from 34 individuals, we found that the receptor-binding domain (RBD) is highly immunogenic and that 33% of RBD-reactive clones and 94% of individuals recognized a conserved immunodominant S346–S365 region comprising nested human leukocyte antigen DR (HLA-DR)– and HLA-DP–restricted epitopes. Using pre– and post–COVID-19 samples and S proteins from endemic coronaviruses, we identified cross-reactive T cells targeting multiple S protein sites. The immunodominant and cross-reactive epitopes identified can inform vaccination strategies to counteract emerging SARS-CoV-2 variants. [1]: /lookup/doi/10.1126/science.abg8985
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/330813
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Jun Siong Low,Daniela Vaqueirinho,Federico Mele,et al. Clonal analysis of immunodominance and cross-reactivity of the CD4 T cell response to SARS-CoV-2[J]. Science,2021.
APA	Jun Siong Low.,Daniela Vaqueirinho.,Federico Mele.,Mathilde Foglierini.,Josipa Jerak.,...&Antonino Cassotta.(2021).Clonal analysis of immunodominance and cross-reactivity of the CD4 T cell response to SARS-CoV-2.Science.
MLA	Jun Siong Low,et al."Clonal analysis of immunodominance and cross-reactivity of the CD4 T cell response to SARS-CoV-2".Science (2021).