MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO

doi:10.1126/science.abg6130

GSTDTAP > 气候变化

DOI	10.1126/science.abg6130
	MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO
	Lucia Ichino; Brandon A. Boone; Luke Strauskulage; C. Jake Harris; Gundeep Kaur; Matthew A. Gladstone; Maverick Tan; Suhua Feng; Yasaman Jami-Alahmadi; Sascha H. Duttke; James A. Wohlschlegel; Xiaodong Cheng; Sy Redding; Steven E. Jacobsen
	2021-06-25
发表期刊	Science
出版年	2021
英文摘要	DNA methylation is a conserved epigenetic mark required for gene silencing in many different organisms. However, how the methyl mark is able to silence genes is still largely unknown. Ichino et al. discovered two Arabidopsis proteins named MBD5 and MBD6 that are recruited to DNA by direct recognition of methylation. These methyl readers recruit the class C J-domain protein SILENZIO to chromatin to silence methylated genes and transposons. SILENZIO likely acts through its interaction with heat shock chaperone proteins. Science , abg6130, this issue p. [1434][1] DNA methylation is associated with transcriptional repression of eukaryotic genes and transposons, but the downstream mechanism of gene silencing is largely unknown. Here, we describe two Arabidopsis thaliana methyl-CpG–binding domain proteins, MBD5 and MBD6, that are recruited to chromatin by recognition of CG methylation, and redundantly repress a subset of genes and transposons without affecting DNA methylation levels. These methyl readers recruit a J-domain protein, SILENZIO, that acts as a transcriptional repressor in loss-of-function and gain-of-function experiments. J-domain proteins often serve as co-chaperones with HSP70s. Indeed, we found that SILENZIO’s conserved J-domain motif was required for its interaction with HSP70s and for its silencing function. These results uncover an unprecedented role of a molecular chaperone J-domain protein in gene silencing downstream of DNA methylation. [1]: /lookup/doi/10.1126/science.abg6130
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/334143
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Lucia Ichino,Brandon A. Boone,Luke Strauskulage,et al. MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO[J]. Science,2021.
APA	Lucia Ichino.,Brandon A. Boone.,Luke Strauskulage.,C. Jake Harris.,Gundeep Kaur.,...&Steven E. Jacobsen.(2021).MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO.Science.
MLA	Lucia Ichino,et al."MBD5 and MBD6 couple DNA methylation to gene silencing through the J-domain protein SILENZIO".Science (2021).