Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes

doi:10.1126/science.abf8705

GSTDTAP > 气候变化

DOI	10.1126/science.abf8705
	Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes
	Nazar Mashtalir; Hai T. Dao; Akshay Sankar; Hengyuan Liu; Aaron J. Corin; John D. Bagert; Eva J. Ge; Andrew R. D’Avino; Martin Filipovski; Brittany C. Michel; Geoffrey P. Dann; Tom W. Muir; Cigall Kadoch
	2021-07-16
发表期刊	Science
出版年	2021
英文摘要	Large, multicomponent molecular machines known as mammalian SWI/SNF (mSWI/SNF) chromatin-remodeling complexes play critical roles in governing the architecture of our genomes. These entities bind to chromatin (DNA assembled on proteins) inside the nucleus and dictate which regions of DNA, and thus which genes in our genome, are made accessible. It is critical that this process happens at the right times and for the right genes in cells, because disruptions cause diseases such as cancers and neurodevelopmental disorders. Mashtalir et al. uncovered molecular cues that direct complex activities on chromatin, informing specific interactions that may be amenable to therapeutic targeting. This combinatorial approach, which considers many factors involved in determining mSWI/SNF activity, provides a valuable resource for understanding the binding and activity of chromatin-remodeling complexes. Science , abf8705, this issue p. [306][1] Mammalian SWI/SNF (mSWI/SNF) adenosine triphosphate–dependent chromatin remodelers modulate genomic architecture and gene expression and are frequently mutated in disease. However, the specific chromatin features that govern their nucleosome binding and remodeling activities remain unknown. We subjected endogenously purified mSWI/SNF complexes and their constituent assembly modules to a diverse library of DNA-barcoded mononucleosomes, performing more than 25,000 binding and remodeling measurements. Here, we define histone modification-, variant-, and mutation-specific effects, alone and in combination, on mSWI/SNF activities and chromatin interactions. Further, we identify the combinatorial contributions of complex module components, reader domains, and nucleosome engagement properties to the localization of complexes to selectively permissive chromatin states. These findings uncover principles that shape the genomic binding and activity of a major chromatin remodeler complex family. [1]: /lookup/doi/10.1126/science.abf8705
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/334379
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Nazar Mashtalir,Hai T. Dao,Akshay Sankar,et al. Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes[J]. Science,2021.
APA	Nazar Mashtalir.,Hai T. Dao.,Akshay Sankar.,Hengyuan Liu.,Aaron J. Corin.,...&Cigall Kadoch.(2021).Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes.Science.
MLA	Nazar Mashtalir,et al."Chromatin landscape signals differentially dictate the activities of mSWI/SNF family complexes".Science (2021).