Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery

doi:10.1126/science.abg6155

GSTDTAP > 气候变化

DOI	10.1126/science.abg6155
	Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
	Michael Segel; Blake Lash; Jingwei Song; Alim Ladha; Catherine C. Liu; Xin Jin; Sergei L. Mekhedov; Rhiannon K. Macrae; Eugene V. Koonin; Feng Zhang
	2021-08-20
发表期刊	Science
出版年	2021
英文摘要	Retroviruses and retroelements have inserted their genetic code into mammalian genomes throughout evolution. Although many of these integrated virus-like sequences pose a threat to genomic integrity, some have been retooled by mammalian cells to perform essential roles in development. Segel et al. found that one of these retroviral-like proteins, PEG10, directly binds to and secretes its own mRNA in extracellular virus–like capsids. These virus-like particles were then pseudotyped with fusogens to deliver functional mRNA cargos to mammalian cells. This potentially provides an endogenous vector for RNA-based gene therapy. Science , abg6155, this issue p. [882][1] Eukaryotic genomes contain domesticated genes from integrating viruses and mobile genetic elements. Among these are homologs of the capsid protein (known as Gag) of long terminal repeat (LTR) retrotransposons and retroviruses. We identified several mammalian Gag homologs that form virus-like particles and one LTR retrotransposon homolog, PEG10, that preferentially binds and facilitates vesicular secretion of its own messenger RNA (mRNA). We showed that the mRNA cargo of PEG10 can be reprogrammed by flanking genes of interest with Peg10 ’s untranslated regions. Taking advantage of this reprogrammability, we developed selective endogenous encapsidation for cellular delivery (SEND) by engineering both mouse and human PEG10 to package, secrete, and deliver specific RNAs. Together, these results demonstrate that SEND is a modular platform suited for development as an efficient therapeutic delivery modality. [1]: /lookup/doi/10.1126/science.abg6155
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/336029
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Michael Segel,Blake Lash,Jingwei Song,et al. Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery[J]. Science,2021.
APA	Michael Segel.,Blake Lash.,Jingwei Song.,Alim Ladha.,Catherine C. Liu.,...&Feng Zhang.(2021).Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery.Science.
MLA	Michael Segel,et al."Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery".Science (2021).