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DOI | 10.1002/joc.4697 |
Cryo-EM structure of a human spliceosome activated for step 2 of splicing | |
Bertram, Karl1; Agafonov, Dmitry E.2; Liu, Wen-Ti1; Dybkov, Olexandr2; Will, Cindy L.2; Hartmuth, Klaus2; Urlaub, Henning3,4; Kastner, Berthold2; Stark, Holger1; Luehrmann, Reinhard2 | |
2017-02-16 | |
发表期刊 | NATURE
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ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 542期号:7641页码:318-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Spliceosome rearrangements facilitated by RNA helicase PRP16 before catalytic step two of splicing are poorly understood. Here we report a 3D cryo-electron microscopy structure of the human spliceosomal C complex stalled directly after PRP16 action (C*). The architecture of the catalytic U2-U6 ribonucleoprotein (RNP) core of the human C* spliceosome is very similar to that of the yeast pre-Prp16 C complex. However, in C* the branched intron region is separated from the catalytic centre by approximately 20 angstrom, and its position close to the U6 small nuclear RNA ACAGA box is stabilized by interactions with the PRP8 RNase H-like and PRP17 WD40 domains. RNA helicase PRP22 is located about 100 angstrom from the catalytic centre, suggesting that it destabilizes the spliced mRNA after step two from a distance. Comparison of the structure of the yeast C and human C* complexes reveals numerous RNP rearrangements that are likely to be facilitated by PRP16, including a large-scale movement of the U2 small nuclear RNP. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000394451600030 |
WOS关键词 | PRE-MESSENGER-RNA ; U4/U6.U5 TRI-SNRNP ; 2ND STEP ; I SPLICEOSOME ; MOLECULAR ARCHITECTURE ; CRYSTAL-STRUCTURE ; CATALYTIC CORE ; PRP8 ; U5 ; INSIGHTS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/37181 |
专题 | 气候变化 |
作者单位 | 1.MPI Biophys Chem, Dept Struct Dynam, Fassberg 11, D-37077 Gottingen, Germany; 2.MPI Biophys Chem, Dept Cellular Biochem, Fassberg 11, D-37077 Gottingen, Germany; 3.MPI Biophys Chem, Bioanalyt Mass Spectrometry, Fassberg 11, D-37077 Gottingen, Germany; 4.Univ Med Ctr Gottingen, Bioanalyt Grp, Inst Clin Chem, Robert Koch Str 40, D-37075 Gottingen, Germany |
推荐引用方式 GB/T 7714 | Bertram, Karl,Agafonov, Dmitry E.,Liu, Wen-Ti,et al. Cryo-EM structure of a human spliceosome activated for step 2 of splicing[J]. NATURE,2017,542(7641):318-+. |
APA | Bertram, Karl.,Agafonov, Dmitry E..,Liu, Wen-Ti.,Dybkov, Olexandr.,Will, Cindy L..,...&Luehrmann, Reinhard.(2017).Cryo-EM structure of a human spliceosome activated for step 2 of splicing.NATURE,542(7641),318-+. |
MLA | Bertram, Karl,et al."Cryo-EM structure of a human spliceosome activated for step 2 of splicing".NATURE 542.7641(2017):318-+. |
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