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DOI | 10.3354/cr01449 |
Mediator structure and rearrangements required for holoenzyme formation | |
Tsai, Kuang-Lei1; Yu, Xiaodi1; Gopalan, Sneha2,3; Chao, Ti-Chun4; Zhang, Ying2,3; Florens, Laurence2,3; Washburn, Michael P.2,3,5; Murakami, Kenji6,8; Conaway, Ronald C.2,3,7; Conaway, Joan W.2,3,7; Asturias, Francisco J.1,9 | |
2017-04-13 | |
发表期刊 | NATURE
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ISSN | 0028-0836 |
EISSN | 1476-4687 |
出版年 | 2017 |
卷号 | 544期号:7649页码:196-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 angstrom resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 angstrom resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000398897900030 |
WOS关键词 | RNA-POLYMERASE-II ; SUBUNIT ARCHITECTURE ; PROTEOMIC ANALYSIS ; TRANSCRIPTION ; INITIATION ; COMPLEX ; YEAST ; IDENTIFICATION ; MICROSCOPY ; VISUALIZATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/38496 |
专题 | 气候变化 |
作者单位 | 1.Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA; 2.Stowers Inst Med Res, Kansas City, MO USA; 3.Univ Calif San Diego, Sch Med, Dept Pediat, La Jolla, CA 92093 USA; 4.Univ Calif San Diego, Sch Med, Inst Genom Med, La Jolla, CA 92093 USA; 5.Univ Kansas, Med Ctr, Dept Pathol & Lab Med, Kansas City, KS 66103 USA; 6.Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA; 7.Univ Kansas, Med Ctr, Dept Biochem & Mol Biol, Kansas City, KS USA; 8.Univ Penn, Dept Biochem & Biophys, Perelman Sch Med, Philadelphia, PA 19104 USA; 9.Univ Colorado, Sch Med, Dept Biochem & Mol Genet, Aurora, CO 80045 USA |
推荐引用方式 GB/T 7714 | Tsai, Kuang-Lei,Yu, Xiaodi,Gopalan, Sneha,et al. Mediator structure and rearrangements required for holoenzyme formation[J]. NATURE,2017,544(7649):196-+. |
APA | Tsai, Kuang-Lei.,Yu, Xiaodi.,Gopalan, Sneha.,Chao, Ti-Chun.,Zhang, Ying.,...&Asturias, Francisco J..(2017).Mediator structure and rearrangements required for holoenzyme formation.NATURE,544(7649),196-+. |
MLA | Tsai, Kuang-Lei,et al."Mediator structure and rearrangements required for holoenzyme formation".NATURE 544.7649(2017):196-+. |
条目包含的文件 | 条目无相关文件。 |
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