MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools

doi:10.2172/1167142

GSTDTAP > 地球科学

DOI	10.2172/1167142
报告编号	DOE-UTK--0004796
来源ID	OSTI ID: 1167142
	MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools
	Loeffler, Frank E.
	2014-12-31
出版年	2014
页数	10
语种	英语
国家	美国
领域	地球科学
英文摘要	Uranium (U) contamination in the subsurface is a major remediation challenge at many DOE sites. Traditional site remedies present enormous costs to DOE; hence, enhanced bioremediation technologies (i.e., biostimulation and bioaugmentation) combined with monitoring efforts are being considered as cost-effective corrective actions to address subsurface contamination. This research effort improved understanding of the microbial U reduction process and developed new tools for monitoring microbial activities. Application of these tools will promote science-based site management decisions that achieve contaminant detoxification, plume control, and long-term stewardship in the most efficient manner. The overarching hypothesis was that the design, validation and application of a suite of new molecular and biogeochemical tools advance process understanding, and improve environmental monitoring regimes to assess and predict in situ U immobilization. Accomplishments: This project (i) advanced nucleic acid-based approaches to elucidate the presence, abundance, dynamics, spatial distribution, and activity of metal- and radionuclide-detoxifying bacteria; (ii) developed proteomics workflows for detection of metal reduction biomarker proteins in laboratory cultures and contaminated site groundwater; (iii) developed and demonstrated the utility of U isotopic fractionation using high precision mass spectrometry to quantify U(VI) reduction for a range of reduction mechanisms and environmental conditions; and (iv) validated the new tools using field samples from U-contaminated IFRC sites, and demonstrated their prognostic and diagnostic capabilities in guiding decision making for environmental remediation and long-term site stewardship.
英文关键词	Uranium microbial activity immobilization monitoring tools
URL	查看原文
来源平台	US Department of Energy (DOE)
引用统计
文献类型	科技报告
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/6632
专题	地球科学
推荐引用方式 GB/T 7714	Loeffler, Frank E.. MURMoT. Design and Application of Microbial Uranium Reduction Monitoring Tools,2014.