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Is the marine ice cliff hypothesis collapsing? 期刊论文
Science, 2021
作者:  Nicholas R. Golledge;  Daniel P. Lowry
收藏  |  浏览/下载:22/0  |  提交时间:2021/06/24
In situ genome sequencing resolves DNA sequence and structure in intact biological samples 期刊论文
Science, 2021
作者:  Andrew C. Payne;  Zachary D. Chiang;  Paul L. Reginato;  Sarah M. Mangiameli;  Evan M. Murray;  Chun-Chen Yao;  Styliani Markoulaki;  Andrew S. Earl;  Ajay S. Labade;  Rudolf Jaenisch;  George M. Church;  Edward S. Boyden;  Jason D. Buenrostro;  Fei Chen
收藏  |  浏览/下载:11/0  |  提交时间:2021/03/02
Illuminating tremors in the deep 期刊论文
Science, 2021
作者:  William Wilcock
收藏  |  浏览/下载:2/0  |  提交时间:2021/03/02
How to improve robotic touch 期刊论文
Science, 2020
作者:  Subramanian Sundaram
收藏  |  浏览/下载:6/0  |  提交时间:2020/11/20
Food for thought 期刊论文
Science, 2020
作者:  Gabor Egervari;  Karl M. Glastad;  Shelley L. Berger
收藏  |  浏览/下载:7/0  |  提交时间:2020/11/09
A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases 期刊论文
NATURE, 2020, 577 (7789) : 244-+
作者:  Mendoza, Senen D.;  Nieweglowska, Eliza S.;  Govindarajan, Sutharsan;  Leon, Lina M.;  Berry, Joel D.;  Tiwari, Anika;  Chaikeeratisak, Vorrapon;  Pogliano, Joe;  Agard, David A.;  Bondy-Denomy, Joseph
收藏  |  浏览/下载:18/0  |  提交时间:2020/07/03

All viruses require strategies to inhibit or evade the immune pathways of cells that they infect. The viruses that infect bacteria, bacteriophages (phages), must avoid immune pathways that target nucleic acids, such as CRISPR-Cas and restriction-modification systems, to replicate efficiently(1). Here we show that jumbo phage phi KZ segregates its DNA from immunity nucleases of its host, Pseudomonas aeruginosa, by constructing a proteinaceous nucleus-like compartment. phi KZ is resistant to many immunity mechanisms that target DNA in vivo, including two subtypes of CRISPR-Cas3, Cas9, Cas12a and the restriction enzymes HsdRMS and EcoRI. Cas proteins and restriction enzymes are unable to access the phage DNA throughout the infection, but engineering the relocalization of EcoRI inside the compartment enables targeting of the phage and protection of host cells. Moreover, phi KZ is sensitive to Cas13a-a CRISPR-Cas enzyme that targets RNA-probably owing to phage mRNA localizing to the cytoplasm. Collectively, we propose that Pseudomonas jumbo phages evade a broad spectrum of DNA-targeting nucleases through the assembly of a protein barrier around their genome.


  
The promise of spatial transcriptomics for neuroscience in the era of molecular cell typing 期刊论文
SCIENCE, 2017, 358 (6359) : 64-+
作者:  Lein, Ed;  Borm, Lars E.;  Linnarsson, Sten
收藏  |  浏览/下载:0/0  |  提交时间:2019/11/27
Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes 期刊论文
SCIENCE, 2017, 355 (6325) : 606-612
作者:  Balzarotti, Francisco;  Eilers, Yvan;  Gwosch, Klaus C.;  Gynna, Arvid H.;  Westphal, Volker;  Stefani, Fernando D.;  Elf, Johan;  Hell, Stefan W.
收藏  |  浏览/下载:5/0  |  提交时间:2019/11/27