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Femtosecond-to-millisecond structural changes in a light-driven sodium pump 期刊论文
NATURE, 2020, 583 (7815) : 314-+
作者:  Moore, Luiza;  Leongamornlert, Daniel;  Coorens, Tim H. H.;  Sanders, Mathijs A.;  Ellis, Peter;  Dentro, Stefan C.;  Dawson, Kevin J.;  Butler, Tim;  Rahbari, Raheleh;  Mitchell, Thomas J.;  Maura, Francesco;  Nangalia, Jyoti;  Tarpey, Patrick S.;  Brunner, Simon F.;  Lee-Six, Henry;  Hooks, Yvette;  Moody, Sarah;  Mahbubani, Krishnaa T.;  Jimenez-Linan, Mercedes;  Brosens, Jan J.;  Iacobuzio-Donahue, Christine A.;  Martincorena, Inigo;  Saeb-Parsy, Kourosh;  Campbell, Peter J.;  Stratton, Michael R.
收藏  |  浏览/下载:17/0  |  提交时间:2020/07/03

Light-driven sodium pumps actively transport small cations across cellular membranes(1). These pumps are used by microorganisms to convert light into membrane potential and have become useful optogenetic tools with applications in neuroscience. Although the resting state structures of the prototypical sodium pump Krokinobacter eikastus rhodopsin 2 (KR2) have been solved(2,3), it is unclear how structural alterations overtime allow sodium to be translocated against a concentration gradient. Here, using the Swiss X-ray Free Electron Laser(4), we have collected serial crystallographic data at ten pump-probe delays from femtoseconds to milliseconds. High-resolution structural snapshots throughout the KR2 photocycle show how retinal isomerization is completed on the femtosecond timescale and changes the local structure of the binding pocket in the early nanoseconds. Subsequent rearrangements and deprotonation of the retinal Schiff base open an electrostatic gate in microseconds. Structural and spectroscopic data, in combination with quantum chemical calculations, indicate that a sodium ion bind stransiently close to the retinal within one millisecond. In the last structural intermediate, at 20 milliseconds after activation, we identified a potential second sodium-binding site close to the extracellular exit. These results provide direct molecular insight into the dynamics of active cation transport across biological membranes.


  
Experimental demonstration of memory-enhanced quantum communication 期刊论文
NATURE, 2020
作者:  Quinn, Robert A.;  Melnik, Alexey, V;  Vrbanac, Alison;  Fu, Ting;  Patras, Kathryn A.;  Christy, Mitchell P.;  Bodai, Zsolt;  Belda-Ferre, Pedro;  Tripathi, Anupriya;  Chung, Lawton K.;  Downes, Michael;  Welch, Ryan D.;  Quinn, Melissa;  Humphrey, Greg;  Panitchpakdi, Morgan;  Weldon, Kelly C.;  Aksenov, Alexander;  da Silva, Ricardo;  Avila-Pacheco, Julian;  Clish, Clary;  Bae, Sena;  Mallick, Himel;  Franzosa, Eric A.;  Lloyd-Price, Jason;  Bussell, Robert;  Thron, Taren;  Nelson, Andrew T.;  Wang, Mingxun;  Leszczynski, Eric;  Vargas, Fernando;  Gauglitz, Julia M.;  Meehan, Michael J.;  Gentry, Emily;  Arthur, Timothy D.;  Komor, Alexis C.;  Poulsen, Orit;  Boland, Brigid S.;  Chang, John T.;  Sandborn, William J.;  Lim, Meerana;  Garg, Neha;  Lumeng, Julie C.;  Xavier, Ramnik J.;  Kazmierczak, Barbara, I;  Jain, Ruchi;  Egan, Marie;  Rhee, Kyung E.;  Ferguson, David;  Raffatellu, Manuela;  Vlamakis, Hera;  Haddad, Gabriel G.;  Siegel, Dionicio;  Huttenhower, Curtis;  Mazmanian, Sarkis K.;  Evans, Ronald M.;  Nizet, Victor;  Knight, Rob;  Dorrestein, Pieter C.
收藏  |  浏览/下载:36/0  |  提交时间:2020/07/03

The ability to communicate quantum information over long distances is of central importance in quantum science and engineering(1). Although some applications of quantum communication such as secure quantum key distribution(2,3) are already being successfully deployed(4-7), their range is currently limited by photon losses and cannot be extended using straightforward measure-and-repeat strategies without compromising unconditional security(8). Alternatively, quantum repeaters(9), which utilize intermediate quantum memory nodes and error correction techniques, can extend the range of quantum channels. However, their implementation remains an outstanding challenge(10-16), requiring a combination of efficient and high-fidelity quantum memories, gate operations, and measurements. Here we use a single solid-state spin memory integrated in a nanophotonic diamond resonator(17-19) to implement asynchronous photonic Bell-state measurements, which are a key component of quantum repeaters. In a proof-of-principle experiment, we demonstrate high-fidelity operation that effectively enables quantum communication at a rate that surpasses the ideal loss-equivalent direct-transmission method while operating at megahertz clock speeds. These results represent a crucial step towards practical quantum repeaters and large-scale quantum networks(20,21).


A solid-state spin memory is used to demonstrate quantum repeater functionality, which has the potential to overcome photon losses involved in long-distance transmission of quantum information.


  
Live-animal imaging of native haematopoietic stem and progenitor cells 期刊论文
NATURE, 2020, 578 (7794) : 278-+
作者:  Gerstung, Moritz;  Jolly, Clemency;  Leshchiner, Ignaty;  Dentro, Stefan C.;  Gonzalez, Santiago;  Rosebrock, Daniel;  Mitchell, Thomas J.;  Rubanova, Yulia;  Anur, Pavana;  Yu, Kaixian;  Tarabichi, Maxime;  Deshwar, Amit;  Wintersinger, Jeff;  Kleinheinz, Kortine;  Vazquez-Garcia, Ignacio;  Haase, Kerstin;  Jerman, Lara;  Sengupta, Subhajit;  Macintyre, Geoff;  Malikic, Salem;  Donmez, Nilgun;  Livitz, Dimitri G.;  Cmero, Marek;  Demeulemeester, Jonas;  Schumacher, Steven;  Fan, Yu;  Yao, Xiaotong;  Lee, Juhee;  Schlesner, Matthias;  Boutros, Paul C.;  Bowtell, David D.;  Zhu, Hongtu;  Getz, Gad;  Imielinski, Marcin;  Beroukhim, Rameen;  Sahinalp, S. Cenk;  Ji, Yuan;  Peifer, Martin;  Markowetz, Florian;  Mustonen, Ville;  Yuan, Ke;  Wang, Wenyi;  Morris, Quaid D.;  Spellman, Paul T.;  Wedge, David C.;  Van Loo, Peter;  Deshwar, Amit G.;  Adams, David J.;  Campbell, Peter J.;  Cao, Shaolong;  Christie, Elizabeth L.;  Cun, Yupeng;  Dawson, Kevin J.;  Drews, Ruben M.;  Eils, Roland;  Fittall, Matthew;  Garsed, Dale W.;  Ha, Gavin;  Lee-Six, Henry;  Martincorena, Inigo;  Oesper, Layla;  Peto, Myron;  Raphael, Benjamin J.;  Salcedo, Adriana;  Shi, Ruian;  Shin, Seung Jun;  Spiro, Oliver;  Stein, Lincoln D.;  Vembu, Shankar;  Wheeler, David A.;  Yang, Tsun-Po
收藏  |  浏览/下载:15/0  |  提交时间:2020/07/03

The biology of haematopoietic stem cells (HSCs) has predominantly been studied under transplantation conditions(1,2). It has been particularly challenging to study dynamic HSC behaviour, given that the visualization of HSCs in the native niche in live animals has not, to our knowledge, been achieved. Here we describe a dual genetic strategy in mice that restricts reporter labelling to a subset of the most quiescent long-term HSCs (LT-HSCs) and that is compatible with current intravital imaging approaches in the calvarial bone marrow(3-5). We show that this subset of LT-HSCs resides close to both sinusoidal blood vessels and the endosteal surface. By contrast, multipotent progenitor cells (MPPs) show greater variation in distance from the endosteum and are more likely to be associated with transition zone vessels. LT-HSCs are not found in bone marrow niches with the deepest hypoxia and instead are found in hypoxic environments similar to those of MPPs. In vivo time-lapse imaging revealed that LT-HSCs at steady-state show limited motility. Activated LT-HSCs show heterogeneous responses, with some cells becoming highly motile and a fraction of HSCs expanding clonally within spatially restricted domains. These domains have defined characteristics, as HSC expansion is found almost exclusively in a subset of bone marrow cavities with bone-remodelling activity. By contrast, cavities with low bone-resorbing activity do not harbour expanding HSCs. These findings point to previously unknown heterogeneity within the bone marrow microenvironment, imposed by the stages of bone turnover. Our approach enables the direct visualization of HSC behaviours and dissection of heterogeneity in HSC niches.


A dual genetic strategy enables the labelling and in vivo imaging of native long-term haematopoietic stem cells in the mouse calvarial bone marrow.


  
Challenging local realism with human choices 期刊论文
NATURE, 2018, 557 (7704) : 212-+
作者:  Abellan, C.;  Acin, A.;  Alarcon, A.;  Alibart, O.;  Andersen, C. K.;  Andreoli, F.;  Beckert, A.;  Beduini, F. A.;  Bendersky, A.;  Bentivegna, M.;  Bierhorst, P.;  Burchardt, D.;  Cabello, A.;  Carine, J.;  Carrasco, S.;  Carvacho, G.;  Cavalcanti, D.;  Chaves, R.;  Cortes-Vega, J.;  Cuevas, A.;  Delgado, A.;  de Riedmatten, H.;  Eichler, C.;  Farrera, P.;  Fuenzalida, J.;  Garcia-Matos, M.;  Garthoff, R.;  Gasparinetti, S.;  Gerrits, T.;  Jouneghani, F. Ghafari;  Glancy, S.;  Gomez, E. S.;  Gonzalez, P.;  Guan, J-Y;  Handsteiner, J.;  Heinsoo, J.;  Heinze, G.;  Hirschmann, A.;  Jimenez, O.;  Kaiser, F.;  Knill, E.;  Knoll, L. T.;  Krinner, S.;  Kurpiers, P.;  Larotonda, M. A.;  Larsson, J-A;  Lenhard, A.;  Li, H.;  Li, M-H;  Lima, G.;  Liu, B.;  Liu, Y.;  Lopez Grande, I. H.;  Lunghi, T.;  Ma, X.;  Magana-Loaiza, O. S.;  Magnard, P.;  Magnoni, A.;  Marti-Prieto, M.;  Martinez, D.;  Mataloni, P.;  Mattar, A.;  Mazzera, M.;  Mirin, R. P.;  Mitchell, M. W.;  Nam, S.;  Oppliger, M.;  Pan, J-W;  Patel, R. B.;  Pryde, G. J.;  Rauch, D.;  Redeker, K.;  Rielander, D.;  Ringbauer, M.;  Roberson, T.;  Rosenfeld, W.;  Salathe, Y.;  Santodonato, L.;  Sauder, G.;  Scheidl, T.;  Schmiegelow, C. T.;  Sciarrino, F.;  Seri, A.;  Shalm, L. K.;  Shi, S-C;  Slussarenko, S.;  Stevens, M. J.;  Tanzilli, S.;  Toledo, F.;  Tura, J.;  Ursin, R.;  Vergyris, P.;  Verma, V. B.;  Walter, T.;  Wallraff, A.;  Wang, Z.;  Weinfurter, H.;  Weston, M. M.;  White, A. G.;  Wu, C.;  Xavier, G. B.;  You, L.;  Yuan, X.;  Zeilinger, A.;  Zhang, Q.;  Zhang, W.;  Zhong, J.
收藏  |  浏览/下载:13/0  |  提交时间:2019/11/27