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Massively multiplexed nucleic acid detection with Cas13 期刊论文
NATURE, 2020, 582 (7811) : 277-+
作者:  Mahato, Biraj;  Kaya, Koray Dogan;  Fan, Yan;  Sumien, Nathalie;  Shetty, Ritu A.;  Zhang, Wei;  Davis, Delaney;  Mock, Thomas;  Batabyal, Subrata;  Ni, Aiguo;  Mohanty, Samarendra;  Han, Zongchao;  Farjo, Rafal;  Forster, Michael J.;  Swaroop, Anand;  Chavala, Sai H.
收藏  |  浏览/下载:84/0  |  提交时间:2020/07/03

CRISPR-based nucleic acid detection is used in a platform that can simultaneously detect 169 human-associated viruses in multiple samples, providing scalable, multiplexed pathogen detection aimed at routine surveillance for public health.


The great majority of globally circulating pathogens go undetected, undermining patient care and hindering outbreak preparedness and response. To enable routine surveillance and comprehensive diagnostic applications, there is a need for detection technologies that can scale to test many samples(1-3)while simultaneously testing for many pathogens(4-6). Here, we develop Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (CARMEN), a platform for scalable, multiplexed pathogen detection. In the CARMEN platform, nanolitre droplets containing CRISPR-based nucleic acid detection reagents(7)self-organize in a microwell array(8)to pair with droplets of amplified samples, testing each sample against each CRISPR RNA (crRNA) in replicate. The combination of CARMEN and Cas13 detection (CARMEN-Cas13) enables robust testing of more than 4,500 crRNA-target pairs on a single array. Using CARMEN-Cas13, we developed a multiplexed assay that simultaneously differentiates all 169 human-associated viruses with at least 10 published genome sequences and rapidly incorporated an additional crRNA to detect the causative agent of the 2020 COVID-19 pandemic. CARMEN-Cas13 further enables comprehensive subtyping of influenza A strains and multiplexed identification of dozens of HIV drug-resistance mutations. The intrinsic multiplexing and throughput capabilities of CARMEN make it practical to scale, as miniaturization decreases reagent cost per test by more than 300-fold. Scalable, highly multiplexed CRISPR-based nucleic acid detection shifts diagnostic and surveillance efforts from targeted testing of high-priority samples to comprehensive testing of large sample sets, greatly benefiting patients and public health(9-11).


  
Quality of Care for PTSD and Depression in the Military Health System: Phase I Report 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Kimberly A. Hepner;  Elizabeth M. Sloss;  Carol P. Roth;  Heather Krull;  Susan M. Paddock;  Shaela Moen;  Martha J. Timmer;  Harold Alan Pincus
收藏  |  浏览/下载:44/0  |  提交时间:2019/04/05
Military Health and Health Care  Depression  Health Care Quality Measurement  Evidence Based Health Practice  Post-Traumatic Stress Disorder  Mental Health Treatment  
Evaluating the role and contribution of innovation to health and wealth in the UK: A review of Innovation, Health and Wealth: Phase 1 Final Report 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Teresa Bienkowska-Gibbs;  Josephine Exley;  Catherine L. Saunders;  Sonja Marjanovic;  Joanna Chataway;  Calum MacLure;  Ruth McDonald;  Tom Ling
收藏  |  浏览/下载:25/0  |  提交时间:2019/04/05
United Kingdom  Health Care Quality  Science, Technology and Innovation Policy  Health Care Program Evaluation  
Racial and Ethnic Differences in Mental Illness Stigma and Discrimination Among Californians Experiencing Mental Health Challenges 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Eunice C. Wong;  Rebecca L. Collins;  Jennifer L. Cerully;  Rachana Seelam;  Elizabeth Roth
收藏  |  浏览/下载:28/0  |  提交时间:2019/04/05
Social Determinants Of Health  Discriminatory Practices  California  Mental Health and Illness  Health Care Program Evaluation  
Galvanising the NHS to adopt innovation: The feasibility and practicality of recommendations from the interim report of the Accelerated Access Review 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Stuart Parris;  Gavin Cochrane;  Sonja Marjanovic;  Tom Ling;  Joanna Chataway
收藏  |  浏览/下载:28/0  |  提交时间:2019/04/05
Health Care Organization and Administration  Organizational Leadership Performance  Science, Technology and Innovation Policyy  Health Care Technology  Organizational Leadership Development  
Evaluation of the Offender Liaison and Diversion Trial Schemes 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Emma Disley;  Celia Taylor;  Kristy Kruithof;  Eleanor Winpenny;  Mark Liddle;  Alex Sutherland;  Richard Lilford;  Sam Wright;  Lyndsay McAteer
收藏  |  浏览/下载:28/0  |  提交时间:2019/04/05
Health Care Access  Law Enforcement  United Kingdom  Social Services and Welfare  Courts  Mental Health and Illness  
Evaluation of CalMHSA Student Mental Health Online Resources 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Lisa Sontag-Padilla;  Courtney Ann Kase;  Michelle W. Woodbridge;  Bradley D. Stein
收藏  |  浏览/下载:16/0  |  提交时间:2019/04/05
The Internet  Students  California  Mental Health and Illness  Health Care Program Evaluation  
Evaluating the role and contribution of innovation to health and wealth in the UK: A review of Innovation, Health and Wealth: Phase 1 Appendix 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Teresa Bienkowska-Gibbs;  Josephine Exley;  Catherine L. Saunders;  Bryn Garrod;  Calum MacLure;  Ruth McDonald;  Tom Ling
收藏  |  浏览/下载:19/0  |  提交时间:2019/04/05
United Kingdom  Health Care Quality  Science, Technology and Innovation Policy  Health Care Program Evaluation  
Building a National Culture of Health: Background, Action Framework, Measures, and Next Steps 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Anita Chandra;  Joie Acosta;  Katherine Grace Carman;  Tamara Dubowitz;  Laura Leviton;  Laurie T. Martin;  Carolyn Miller;  Christopher Nelson;  Tracy Orleans;  Margaret Tait;  et al.
收藏  |  浏览/下载:37/0  |  提交时间:2019/04/05
Health Interventions  Health Care Costs  Health Behaviors  United States  Chronic Diseases and Conditions  Health and Wellness Promotion  Health-related Quality Of Life  Community Health  
Ten Frequently Asked Questions About Veterans' Transitions: Results of a Decade of RAND Work on Veteran Life 科技报告
来源:Rand Corporation. 出版年: 2016
作者:  Christopher Guo;  Julia Pollak;  Melissa Bauman
收藏  |  浏览/下载:11/0  |  提交时间:2019/04/05
Veterans' Education  Suicide  Workplace Discrimination  Post-Traumatic Stress Disorder  Veteran Health Care  Employment and Unemployment  Mental Health Treatment  Students