GSTDTAP

浏览/检索结果: 共10条,第1-10条 帮助

已选(0)清除 条数/页:   排序方式:
Insights into the assembly and activation of the microtubule nucleator gamma-TuRC 期刊论文
NATURE, 2020, 578 (7795) : 467-+
作者:  Cyranoski, David
收藏  |  浏览/下载:39/0  |  提交时间:2020/07/03

Microtubules are dynamic polymers of alpha- and beta-tubulin and have crucial roles in cell signalling, cell migration, intracellular transport and chromosome segregation(1). They assemble de novo from alpha beta-tubulin dimers in an essential process termed microtubule nucleation. Complexes that contain the protein gamma-tubulin serve as structural templates for the microtubule nucleation reaction(2). In vertebrates, microtubules are nucleated by the 2.2-megadalton gamma-tubulin ring complex (gamma-TuRC), which comprises gamma-tubulin, five related gamma-tubulin complex proteins (GCP2-GCP6) and additional factors(3). GCP6 is unique among the GCP proteins because it carries an extended insertion domain of unknown function. Our understanding of microtubule formation in cells and tissues is limited by a lack of high-resolution structural information on the gamma-TuRC. Here we present the cryo-electron microscopy structure of gamma-TuRC from Xenopus laevis at 4.8 angstrom global resolution, and identify a 14-spoked arrangement of GCP proteins and gamma-tubulins in a partially flexible open left-handed spiral with a uniform sequence of GCP variants. By forming specific interactions with other GCP proteins, the GCP6-specific insertion domain acts as a scaffold for the assembly of the gamma-TuRC. Unexpectedly, we identify actin as a bona fide structural component of the gamma-TuRC with functional relevance in microtubule nucleation. The spiral geometry of gamma-TuRC is suboptimal for microtubule nucleation and a controlled conformational rearrangement of the gamma-TuRC is required for its activation. Collectively, our cryo-electron microscopy reconstructions provide detailed insights into the molecular organization, assembly and activation mechanism of vertebrate gamma-TuRC, and will serve as a framework for the mechanistic understanding of fundamental biological processes associated with microtubule nucleation, such as meiotic and mitotic spindle formation and centriole biogenesis(4).


The cryo-EM structure of the gamma-tubulin ring complex (gamma-TuRC) from Xenopus laevis provides insights into the molecular organization of the complex, and shows that actin is a structural component that is functionally relevant to microtubule nucleation.


  
Streamflow Reconstruction in the Upper Missouri River Basin Using a Novel Bayesian Network Model 期刊论文
WATER RESOURCES RESEARCH, 2019, 55 (9) : 7694-7716
作者:  Ravindranath, Arun;  Devineni, Naresh;  Lall, Upmanu;  Cook, Edward R.;  Pederson, Greg;  Martin, Justin;  Woodhouse, Connie
收藏  |  浏览/下载:20/0  |  提交时间:2019/11/27
spatial Markov model  paleo-reconstructions  streamflow reconstructions  Bayesian statistics  water management  stochastic hydrology  
European warm-season temperature and hydroclimate since 850 CE 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2019, 14 (8)
作者:  Ljungqvist, Fredrik Charpentier;  Seim, Andrea;  Krusic, Paul J.;  Gonzalez-Rouco, Jesus Fidel;  Werner, Johannes P.;  Cook, Edward R.;  Zorita, Eduardo;  Luterbacher, Juerg;  Xoplaki, Elena;  Destouni, Georgia;  Garcia-Bustainante, Elena;  Aguilar, Camilo Andres Melo;  Seftigen, Kristina;  Wang, Jianglin;  Gagen, Mary H.;  Esper, Jan;  Solomina, Olga;  Fleitmann, Dominik;  Buntgen, Ulf
收藏  |  浏览/下载:21/0  |  提交时间:2019/11/27
climate variability  climate model simulations  gridded climate reconstructions  hydroclimate  Europe  past millennium  tree-ring data  
Persistent Draining of the Stratospheric Be-10 Reservoir After the Samalas Volcanic Eruption (1257 CE) 期刊论文
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES, 2019, 124 (13) : 7082-7097
作者:  Baroni, Melanie;  Bard, Edouard;  Petit, Jean-Robert;  Viseur, Sophie;  Aumaitre, Georges;  Bourles, Didier L.;  Keddadouche, Karim
收藏  |  浏览/下载:23/0  |  提交时间:2019/11/27
beryllium-10  ice cores  stratospheric volcanic eruptions  Samalas eruption  solar reconstructions  
Multidecadal to centennial surface wintertime wind variability over Northeastern North America via statistical downscaling 期刊论文
CLIMATE DYNAMICS, 2019, 53: 41-66
作者:  Lucio-Eceiza, Etor E.;  Fidel Gonzalez-Rouco, J.;  Garcia-Bustamante, Elena;  Navarro, Jorge;  Beltrami, Hugo
收藏  |  浏览/下载:18/0  |  提交时间:2019/11/27
Surface wind  Statistical downscaling  Spatial and temporal variability  Sensitivity  Reanalysis intercomparison  Past reconstructions  Extreme events  
Quantitative Holocene climatic reconstructions for the lower Yangtze region of China 期刊论文
CLIMATE DYNAMICS, 2018, 50: 1101-1113
作者:  Li, Jianyong;  Dodson, John;  Yan, Hong;  Wang, Weiming;  Innes, James B.;  Zong, Yongqiang;  Zhang, Xiaojian;  Xu, Qinghai;  Ni, Jian;  Lu, Fengyan
收藏  |  浏览/下载:21/0  |  提交时间:2019/04/09
Lower Yangtze  China  Holocene  Climate  Pollen  Quantitative reconstructions  
A 250-year drought catalogue for the island of Ireland (1765-2015) 期刊论文
INTERNATIONAL JOURNAL OF CLIMATOLOGY, 2017, 37
作者:  Noone, S.;  Broderick, C.;  Duffy, C.;  Matthews, T.;  Wilby, R. L.;  Murphy, C.
收藏  |  浏览/下载:24/0  |  提交时间:2019/04/09
drought  Standardized Precipitation Index  documentary sources  newspaper archives  climate reconstructions  Ireland  
Tropospheric circulation during the early twentieth century Arctic warming 期刊论文
CLIMATE DYNAMICS, 2017, 48
作者:  Wegmann, Martin;  Bronnimann, Stefan;  Compo, Gilbert P.
收藏  |  浏览/下载:12/0  |  提交时间:2019/04/09
Arctic climate  Arctic warming  Circulation  Reanalysis  Reconstructions  Troposphere  Climate change  Heat flux  
Reconstructed and simulated temperature asymmetry between continents in both hemispheres over the last centuries 期刊论文
CLIMATE DYNAMICS, 2017, 48
作者:  Goosse, Hugues
收藏  |  浏览/下载:6/0  |  提交时间:2019/04/09
Past millennium  Continental-scale temperature changes  PAGES 2k reconstructions  Data assimilation  Interhemispheric contrasts  
Assessing reconstruction techniques of the Atlantic Ocean circulation variability during the last millennium 期刊论文
CLIMATE DYNAMICS, 2017, 48
作者:  Moreno-Chamarro, Eduardo;  Ortega, Pablo;  Gonzalez-Rouco, Fidel;  Montoya, Marisa
收藏  |  浏览/下载:16/0  |  提交时间:2019/04/09
Last millennium  Little Ice Age  Pseudo-reconstructions  AMOC  Florida Current  Thermal-wind transport