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Deciphering human macrophage development at single-cell resolution 期刊论文
NATURE, 2020
作者:  Oberst, Polina;  Fievre, Sabine;  Baumann, Natalia;  Concetti, Cristina;  Bartolini, Giorgia;  Jabaudon, Denis
收藏  |  浏览/下载:38/0  |  提交时间:2020/07/03

Macrophages are the first cells of the nascent immune system to emerge during embryonic development. In mice, embryonic macrophages infiltrate developing organs, where they differentiate symbiotically into tissue-resident macrophages (TRMs)(1). However, our understanding of the origins and specialization of macrophages in human embryos is limited. Here we isolated CD45(+) haematopoietic cells from human embryos at Carnegie stages 11 to 23 and subjected them to transcriptomic profiling by single-cell RNA sequencing, followed by functional characterization of a population of CD45(+)CD34(+)CD44(+) yolk sac-derived myeloid-biased progenitors (YSMPs) by single-cell culture. We also mapped macrophage heterogeneity across multiple anatomical sites and identified diverse subsets, including various types of embryonic TRM (in the head, liver, lung and skin). We further traced the specification trajectories of TRMs from either yolk sac-derived primitive macrophages or YSMP-derived embryonic liver monocytes using both transcriptomic and developmental staging information, with a focus on microglia. Finally, we evaluated the molecular similarities between embryonic TRMs and their adult counterparts. Our data represent a comprehensive characterization of the spatiotemporal dynamics of early macrophage development during human embryogenesis, providing a reference for future studies of the development and function of human TRMs.


Single-cell RNA sequencing of haematopoietic cells from human embryos at different developmental stages sheds light on the development and specification of macrophages in different tissues.


  
Ecological characteristics of floodplain forest reference sites in the Upper Mississippi River System 期刊论文
FOREST ECOLOGY AND MANAGEMENT, 2018, 427: 208-216
作者:  Guyon, Lyle J.;  Battaglia, Loretta L.
收藏  |  浏览/下载:13/0  |  提交时间:2019/04/09
Upper Mississippi River  Floodplain forests  Ecological characteristics  Restoration  Reference sites  
Radiochemically-Supported Microbial Communities: A Potential Mechanism for Biocolloid Production of Importance to Actinide Transport 科技报告
来源:US Department of Energy (DOE). 出版年: 2014
作者:  Moser, Duane P;  Hamilton-Brehm, Scott D;  Fisher, Jenny C;  Bruckner, James C;  Kruger, Brittany;  Sackett, Joshua;  Russell, Charles E;  Onstott, Tullis C;  Czerwinski, Ken
收藏  |  浏览/下载:42/0  |  提交时间:2019/04/05
Due to the legacy of Cold War nuclear weapons testing  the Nevada National Security Site (NNSS  formerly known as the Nevada Test Site (NTS)) contains millions of Curies of radioactive contamination. Presented here is a summary of the results of the first comprehensive study of subsurface microbial communities of radioactive and nonradioactive aquifers at this site. To achieve the objectives of this project  cooperative actions between the Desert Research Institute (DRI)  the Nevada Field Office of the National Nuclear Security Administration (NNSA)  the Underground Test Area Activity (UGTA)  and contractors such as Navarro-Interra (NI)  were required. Ultimately  fluids from 17 boreholes and two water-filled tunnels were sampled (sometimes on multiple occasions and from multiple depths) from the NNSS  the adjacent Nevada Test and Training Range (NTTR)  and a reference hole in the Amargosa Valley near Death Valley. The sites sampled ranged from highly-radioactive nuclear device test cavities to uncontaminated perched and regional aquifers. Specific areas sampled included recharge  intermediate  and discharge zones of a 100  000-km2 internally-draining province  known as the Death Valley Regional Flow System (DVRFS)  which encompasses the entirety of the NNSS/NTTR and surrounding areas. Specific geological features sampled included: West Pahute and Ranier Mesas (recharge zone)  Yucca and Frenchman Flats (transitional zone)  and the Western edge of the Amargosa Valley near Death Valley (discharge zone). The original overarching question underlying the proposal supporting this work was stated as: Can radiochemically-produced substrates support indigenous microbial communities and subsequently stimulate biocolloid formation that can affect radionuclides in NNSS subsurface nuclear test/detonation sites? Radioactive and non-radioactive groundwater samples were thus characterized for physical parameters  aqueous geochemistry  and microbial communities using both DNA- and cultivation-based tools in an effort to understand the drivers of microbial community structure (including radioactivity) and microbial interactions with select radionuclides and other factors across the range of habitats surveyed.