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Spatial resolution and location impact group structure in a marine food web 期刊论文
ECOLOGY LETTERS, 2020
作者:  Ohlsson, Mikael;  Eklof, Anna
收藏  |  浏览/下载:14/0  |  提交时间:2020/07/14
Communities  ecological networks  food webs  group model  group structure  spatial location  spatial resolution  
Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme 期刊论文
NATURE, 2020, 581 (7808) : 323-+
作者:  Nikoo, Mohammad Samizadeh;  Jafari, Armin;  Perera, Nirmana;  Zhu, Minghua;  Santoruvo, Giovanni;  Matioli, Elison
收藏  |  浏览/下载:36/0  |  提交时间:2020/07/03

Triacylglycerols store metabolic energy in organisms and have industrial uses as foods and fuels. Excessive accumulation of triacylglycerols in humans causes obesity and is associated with metabolic diseases(1). Triacylglycerol synthesis is catalysed by acyl-CoA diacylglycerol acyltransferase (DGAT) enzymes(2-4), the structures and catalytic mechanisms of which remain unknown. Here we determined the structure of dimeric human DGAT1, a member of the membrane-bound O-acyltransferase (MBOAT) family, by cryo-electron microscopy at approximately 3.0 angstrom resolution. DGAT1 forms a homodimer through N-terminal segments and a hydrophobic interface, with putative active sites within the membrane region. A structure obtained with oleoyl-CoA substrate resolved at approximately 3.2 angstrom shows that the CoA moiety binds DGAT1 on the cytosolic side and the acyl group lies deep within a hydrophobic channel, positioning the acyl-CoA thioester bond near an invariant catalytic histidine residue. The reaction centre is located inside a large cavity, which opens laterally to the membrane bilayer, providing lipid access to the active site. A lipid-like density-possibly representing an acyl-acceptor molecule-is located within the reaction centre, orthogonal to acyl-CoA. Insights provided by the DGAT1 structures, together with mutagenesis and functional studies, provide the basis for a model of the catalysis of triacylglycerol synthesis by DGAT.


Cryo-electron microscopy structures and functional and mutagenesis studies provide insights into the catalysis of triacylglycerol synthesis by human acyl-CoA diacylglycerol acyltransferase at its intramembrane active site.


  
A new perspective to promote low-carbon consumption: The influence of reference groups 期刊论文
ECOLOGICAL ECONOMICS, 2019, 161: 100-108
作者:  Mi, Lingyun;  Zhu, Hanlin;  Yang, Jie;  Gan, Xiaoli;  Xu, Ting;  Qiao, Lijie;  Liu, Qingyan
收藏  |  浏览/下载:22/0  |  提交时间:2019/11/27
Low-carbon consumption  Reference group  Structural equation model  Multi-group analysis  
The coevolution of economic institutions and sustainable consumption via cultural group selection 期刊论文
ECOLOGICAL ECONOMICS, 2017, 131
作者:  Waring, Timothy M.;  Goff, Sandra H.;  Smaldino, Paul E.
收藏  |  浏览/下载:20/0  |  提交时间:2019/04/09
Cultural group selection  Institutions  Conservation  Cooperation  Multilevel selection model  
Feasibility Study of Economics and Performance of Solar Photovoltaics at the VAG Mine Site in Eden and Lowell, Vermont. A Study Prepared in Partnership with the Environmental Protection Agency for the RE-Powering America's Land Initiative: Siting Renewabl 科技报告
来源:US Department of Energy (DOE). 出版年: 2013
作者:  Simon, J.;  Mosey, G.
收藏  |  浏览/下载:19/0  |  提交时间:2019/04/05
EPA  RE-POWERING AMERICA'S LAND  PV  PHOTOVOLTAIC  VAG  VERMONT ASBESTOS GROUP  EDEN  LOWELL  VERMONT  RENEWABLE ENERGY  FINANCING  ECONOMICS  SAM  SYSTEM ADVISOR MODEL  JEDI  MINE  Solar Energy - Photovoltaics  Energy Analysis  Market Transformation