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Structure and mechanism of human diacylglycerol O-acyltransferase 1 期刊论文
NATURE, 2020, 581 (7808) : 329-+
作者:  Wu, Fan;  Zhao, Su;  Yu, Bin;  Chen, Yan-Mei;  Wang, Wen;  Song, Zhi-Gang;  Hu, Yi;  Tao, Zhao-Wu;  Tian, Jun-Hua;  Pei, Yuan-Yuan;  Yuan, Ming-Li;  Zhang, Yu-Ling;  Dai, Fa-Hui;  Liu, Yi;  Wang, Qi-Min;  Zheng, Jiao-Jiao;  Xu, Lin;  Holmes, Edward C.;  Zhang, Yong-Zhen
收藏  |  浏览/下载:57/0  |  提交时间:2020/07/03

The structure of human diacylglycerol O-acyltransferase 1, a membrane protein that synthesizes triacylglycerides, is solved with cryo-electron microscopy, providing insight into its function and mechanism of enzymatic activity.


Diacylglycerol O-acyltransferase 1 (DGAT1) synthesizes triacylglycerides and is required for dietary fat absorption and fat storage in humans(1). DGAT1 belongs to the membrane-bound O-acyltransferase (MBOAT) superfamily, members of which are found in all kingdoms of life and are involved in the acylation of lipids and proteins(2,3). How human DGAT1 and other mammalian members of the MBOAT family recognize their substrates and catalyse their reactions is unknown. The absence of three-dimensional structures also hampers rational targeting of DGAT1 for therapeutic purposes. Here we present the cryo-electron microscopy structure of human DGAT1 in complex with an oleoyl-CoA substrate. Each DGAT1 protomer has nine transmembrane helices, eight of which form a conserved structural fold that we name the MBOAT fold. The MBOAT fold in DGAT1 forms a hollow chamber in the membrane that encloses highly conserved catalytic residues. The chamber has separate entrances for each of the two substrates, fatty acyl-CoA and diacylglycerol. DGAT1 can exist as either a homodimer or a homotetramer and the two forms have similar enzymatic activity. The N terminus of DGAT1 interacts with the neighbouring protomer and these interactions are required for enzymatic activity.


  
Spatial inequality in the context of city-suburb cleavages-Enlarging the framework of well-being and social inequality 期刊论文
LANDSCAPE AND URBAN PLANNING, 2018, 177: 328-339
作者:  Frenkel, Amnon;  Israel, Emil
收藏  |  浏览/下载:15/0  |  提交时间:2019/04/09
Social inequality  City-suburb  Capital forms  Capabilities  Life chances  
Bryophytes as bioindicators of the atmospheric environment in urban-forest landscapes 期刊论文
LANDSCAPE AND URBAN PLANNING, 2017, 167
作者:  Oishi, Yoshitaka;  Hiura, Tsutom
收藏  |  浏览/下载:22/0  |  提交时间:2019/04/09
Bioindicator  Hygrophilous life-forms  Index of atmospheric purity  Land use  Nitrogen pollution  Urban heat island  
Contrasting responses of leaf stomatal characteristics to climate change: a considerable challenge to predict carbon and water cycles 期刊论文
GLOBAL CHANGE BIOLOGY, 2017, 23 (9)
作者:  Yan, Weiming;  Zhong, Yangquanwei;  Shangguan, Zhouping
收藏  |  浏览/下载:16/0  |  提交时间:2019/04/09
carbon and water cycles  climate change  drought stress  elevated CO2 concentration  elevated temperature  life forms  stomatal frequency