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Structural basis of energy transfer in Porphyridium purpureum phycobilisome 期刊论文
NATURE, 2020
作者:  Long, Haizhen;  Zhang, Liwei;  Lv, Mengjie;  Wen, Zengqi;  Zhang, Wenhao;  Chen, Xiulan;  Zhang, Peitao;  Li, Tongqing;  Chang, Luyuan;  Jin, Caiwei;  Wu, Guozhao;  Wang, Xi;  Yang, Fuquan;  Pei, Jianfeng;  Chen, Ping;  Margueron, Raphael;  Deng, Haiteng;  Zhu, Mingzhao;  Li, Guohong
收藏  |  浏览/下载:45/0  |  提交时间:2020/07/03

The cryo-electron microscopy structure of a phycobilisome from the red alga Porphyridium purpureum reveals how aromatic interactions between the linker proteins and the chromophores drive a unidirectional transfer of energy.


Photosynthetic organisms have developed various light-harvesting systems to adapt to their environments(1). Phycobilisomes are large light-harvesting protein complexes found in cyanobacteria and red algae(2-4), although how the energies of the chromophores within these complexes are modulated by their environment is unclear. Here we report the cryo-electron microscopy structure of a 14.7-megadalton phycobilisome with a hemiellipsoidal shape from the red alga Porphyridium purpureum. Within this complex we determine the structures of 706 protein subunits, including 528 phycoerythrin, 72 phycocyanin, 46 allophycocyanin and 60 linker proteins. In addition, 1,598 chromophores are resolved comprising 1,430 phycoerythrobilin, 48 phycourobilin and 120 phycocyanobilin molecules. The markedly improved resolution of our structure compared with that of the phycobilisome of Griffithsia pacifica(5) enabled us to build an accurate atomic model of the P. purpureum phycobilisome system. The model reveals how the linker proteins affect the microenvironment of the chromophores, and suggests that interactions of the aromatic amino acids of the linker proteins with the chromophores may be a key factor in fine-tuning the energy states of the chromophores to ensure the efficient unidirectional transfer of energy.


  
An orbital water-ice cycle on comet 67P from colour changes 期刊论文
NATURE, 2020, 578 (7793) : 49-+
作者:  Oh, Myoung Hwan;  Cho, Min Gee;  Chung, Dong Young;  Park, Inchul;  Kwon, Youngwook Paul;  Ophus, Colin;  Kim, Dokyoon;  Kim, Min Gyu;  Jeong, Beomgyun;  Gu, X. Wendy;  Jo, Jinwoung;  Yoo, Ji Mun;  Hong, Jaeyoung;  McMains, Sara;  Kang, Kisuk;  Sung, Yung-Eun;  Alivisatos, A. Paul;  Hyeon, Taeghwan
收藏  |  浏览/下载:75/0  |  提交时间:2020/07/03

Solar heating of a cometary surface provides the energy necessary to sustain gaseous activity, through which dust is removed(1,2). In this dynamical environment, both the coma(3,4) and the nucleus(5,6) evolve during the orbit, changing their physical and compositional properties. The environment around an active nucleus is populated by dust grains with complex and variegated shapes(7), lifted and diffused by gases freed from the sublimation of surface ices(8,9). The visible colour of dust particles is highly variable: carbonaceous organic material-rich grains(10) appear red while magnesium silicate-rich(11,12) and water-ice-rich(13,14) grains appear blue, with some dependence on grain size distribution, viewing geometry, activity level and comet family type. We know that local colour changes are associated with grain size variations, such as in the bluer jets made of submicrometre grains on comet Hale-Bopp(15) or in the fragmented grains in the coma(16) of C/1999 S4 (LINEAR). Apart from grain size, composition also influences the coma'  s colour response, because transparent volatiles can introduce a substantial blueing in scattered light, as observed in the dust particles ejected after the collision of the Deep Impact probe with comet 9P/Tempel 1(17). Here we report observations of two opposite seasonal colour cycles in the coma and on the surface of comet 67P/Churyumov-Gerasimenko through its perihelion passage(18). Spectral analysis indicates an enrichment of submicrometre grains made of organic material and amorphous carbon in the coma, causing reddening during the passage. At the same time, the progressive removal of dust from the nucleus causes the exposure of more pristine and bluish icy layers on the surface. Far from the Sun, we find that the abundance of water ice on the nucleus is reduced owing to redeposition of dust and dehydration of the surface layer while the coma becomes less red.


  
Phenological mismatch with trees reduces wildflower carbon budgets 期刊论文
ECOLOGY LETTERS, 2019, 22 (4) : 616-623
作者:  Heberling, J. Mason;  MacKenzie, Caitlin McDonough;  Fridley, Jason D.;  Kalisz, Susan;  Primack, Richard B.
收藏  |  浏览/下载:21/0  |  提交时间:2019/04/09
climate change  carbon gain  forest understorey  herbaceous layer  light environment  phenology  spring wildflowers  Thoreau  
Why artificial light at night should be a focus for global change research in the 21st century 期刊论文
GLOBAL CHANGE BIOLOGY, 2018, 24 (3) : 872-882
作者:  Davies, Thomas W.;  Smyth, Tim
收藏  |  浏览/下载:12/0  |  提交时间:2019/04/09
artificial light at night  ecology  global change  human health  human-environment interrelationships  
Assessment of end-of-life design in solid-state lighting 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2017, 12 (8)
作者:  Dzombak, Rachel;  Padon, Jack;  Salsbury, Josh;  Dillon, Heather
收藏  |  浏览/下载:7/0  |  提交时间:2019/04/09
end of life  lighting  green design  design for environment  light-emitting diode  recycling  solid-state lighting