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Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping 期刊论文
NATURE, 2020, 583 (7817) : 638-+
作者:  Lin, Yiheng;  Leibrandt, David R.;  Leibfriedz, Dietrich;  Chou, Chin-wen
收藏  |  浏览/下载:42/0  |  提交时间:2020/07/03

A method termed ac(4)C-seq is introduced for the transcriptome-wide mapping of the RNA modificationN(4)-acetylcytidine, revealing widespread temperature-dependent acetylation that facilitates thermoadaptation in hyperthermophilic archaea.


N-4-acetylcytidine (ac(4)C) is an ancient and highly conserved RNA modification that is present on tRNA and rRNA and has recently been investigated in eukaryotic mRNA(1-3). However, the distribution, dynamics and functions of cytidine acetylation have yet to be fully elucidated. Here we report ac(4)C-seq, a chemical genomic method for the transcriptome-wide quantitative mapping of ac(4)C at single-nucleotide resolution. In human and yeast mRNAs, ac(4)C sites are not detected but can be induced-at a conserved sequence motif-via the ectopic overexpression of eukaryotic acetyltransferase complexes. By contrast, cross-evolutionary profiling revealed unprecedented levels of ac(4)C across hundreds of residues in rRNA, tRNA, non-coding RNA and mRNA from hyperthermophilic archaea. (AcC)-C-4 is markedly induced in response to increases in temperature, and acetyltransferase-deficient archaeal strains exhibit temperature-dependent growth defects. Visualization of wild-type and acetyltransferase-deficient archaeal ribosomes by cryo-electron microscopy provided structural insights into the temperature-dependent distribution of ac(4)C and its potential thermoadaptive role. Our studies quantitatively define the ac(4)C landscape, providing a technical and conceptual foundation for elucidating the role of this modification in biology and disease(4-6).


  
Performance-limiting nanoscale trap clusters at grain junctions in halide perovskites 期刊论文
NATURE, 2020, 580 (7803) : 360-+
作者:  van den Brink, Susanne C.;  Alemany, Anna;  van Batenburg, Vincent;  Moris, Naomi;  Blotenburg, Marloes;  Vivie, Judith;  Baillie-Johnson, Peter;  Nichols, Jennifer;  Sonnen, Katharina F.;  Arias, Alfonso;  van Oudenaarden, Alexander
收藏  |  浏览/下载:39/0  |  提交时间:2020/07/03

Halide perovskite materials have promising performance characteristics for low-cost optoelectronic applications. Photovoltaic devices fabricated from perovskite absorbers have reached power conversion efficiencies above 25 per cent in single-junction devices and 28 per cent in tandem devices(1,2). This strong performance (albeit below the practical limits of about 30 per cent and 35 per cent, respectively(3)) is surprising in thin films processed from solution at low-temperature, a method that generally produces abundant crystalline defects(4). Although point defects often induce only shallow electronic states in the perovskite bandgap that do not affect performance(5), perovskite devices still have many states deep within the bandgap that trap charge carriers and cause them to recombine non-radiatively. These deep trap states thus induce local variations in photoluminescence and limit the device performance(6). The origin and distribution of these trap states are unknown, but they have been associated with light-induced halide segregation in mixed-halide perovskite compositions(7) and with local strain(8), both of which make devices less stable(9). Here we use photoemission electron microscopy to image the trap distribution in state-of-the-art halide perovskite films. Instead of a relatively uniform distribution within regions of poor photoluminescence efficiency, we observe discrete, nanoscale trap clusters. By correlating microscopy measurements with scanning electron analytical techniques, we find that these trap clusters appear at the interfaces between crystallographically and compositionally distinct entities. Finally, by generating time-resolved photoemission sequences of the photo-excited carrier trapping process(10,11), we reveal a hole-trapping character with the kinetics limited by diffusion of holes to the local trap clusters. Our approach shows that managing structure and composition on the nanoscale will be essential for optimal performance of halide perovskite devices.


  
Observation of the Kondo screening cloud 期刊论文
NATURE, 2020, 579 (7798) : 210-+
作者:  Shahnawaz, Mohammad;  Mukherjee, Abhisek;  Pritzkow, Sandra;  Mendez, Nicolas;  Rabadia, Prakruti;  Liu, Xiangan;  Hu, Bo;  Schmeichel, Ann;  Singer, Wolfgang;  Wu, Gang;  Tsai, Ah-Lim;  Shirani, Hamid;  Nilsson, K. Peter R.;  Low, Phillip A.;  Soto, Claudio
收藏  |  浏览/下载:21/0  |  提交时间:2020/07/03

When a magnetic impurity exists in a metal, conduction electrons form a spin cloud that screens the impurity spin. This basic phenomenon is called the Kondo effect(1,2). Unlike electric-charge screening, the spin-screening cloud(3-6) occurs quantum coherently, forming spin-singlet entanglement with the impurity. Although the spins interact locally around the impurity, the Kondo cloud can theoretically spread out over several micrometres. The cloud has not so far been detected, and so its physical existence-a fundamental aspect of the Kondo effect-remains controversial(7,8). Here we present experimental evidence of a Kondo cloud extending over a length of micrometres, comparable to the theoretical length xi(K). In our device, a Kondo impurity is formed in a quantum dot(2,9-11), coupling on one side to a quasi-one-dimensional channel(12) that houses a Fabry-Perot interferometer of various gate-defined lengths L exceeding one micrometre. When we sweep a voltage on the interferometer end gate-separated by L from the quantum dot-to induce Fabry-Perot oscillations in conductance we observe oscillations in the measured Kondo temperature T-K, which is a signature of the Kondo cloud at distance L. When L is less than xi(K) the T-K oscillation amplitude becomes larger as L becomes smaller, obeying a scaling function of a single parameter L/xi(K), whereas when L is greater than xi(K) the oscillation is much weaker. Our results reveal that xi(K) is the only length parameter associated with the Kondo effect, and that the cloud lies mostly within a length of xi(K). Our experimental method offers a way of detecting the spatial distribution of exotic non-Fermi liquids formed by multiple magnetic impurities or multiple screening channels(13-16) and of studying spin-correlated systems.


  
Effects of Memory Biases on Variability of Temperature Reconstructions 期刊论文
JOURNAL OF CLIMATE, 2019, 32 (24) : 8713-8731
作者:  Lucke, Lucie J.;  Hegerl, Gabriele C.;  Schurer, Andrew;  Wilson, Rob
收藏  |  浏览/下载:19/0  |  提交时间:2020/02/17
Surface temperature  Tree rings  Spectral analysis  models  distribution  Hindcasts  Climate variability  
Changes in daily temperature extremes relative to the mean in Coupled Model Intercomparison Project Phase 5 models and observations 期刊论文
INTERNATIONAL JOURNAL OF CLIMATOLOGY, 2019, 39 (14) : 5273-5291
作者:  Gross, Mia H.;  Donat, Markus G.;  Alexander, Lisa V.
收藏  |  浏览/下载:20/0  |  提交时间:2020/02/17
cold extremes  global  HadGHCND  hot extremes  mean temperature  temperature distribution  
Distribution and temporal trends of temperature extremes over Antarctica 期刊论文
ENVIRONMENTAL RESEARCH LETTERS, 2019, 14 (8)
作者:  Wei, Ting;  Yan, Qing;  Ding, Minghu
收藏  |  浏览/下载:16/0  |  提交时间:2019/11/27
temperature extremes  Antarctica  spatial distribution  temporal trend  
Whales in warming water: Assessing breeding habitat diversity and adaptability in Oceania's changing climate 期刊论文
GLOBAL CHANGE BIOLOGY, 2019, 25 (4) : 1466-1481
作者:  Derville, Solene;  Torres, Leigh G.;  Albertson, Renee;  Andrews, Olive;  Baker, C. Scott;  Carzon, Pamela;  Constantine, Rochelle;  Donoghue, Michael;  Dutheil, Cyril;  Gannier, Alexandre;  Oremus, Marc;  Poole, Michael M.;  Robbins, Jooke;  Garrigue, Claire
收藏  |  浏览/下载:28/0  |  提交时间:2019/04/09
climate change  habitat modeling  humpback whales  Oceania  prediction  sea surface temperature  seamounts  species distribution  
Heterogeneity of Scaling of the Observed Global Temperature Data 期刊论文
JOURNAL OF CLIMATE, 2019, 32 (2) : 349-367
作者:  Blesic, Suzana;  Zanchettin, Davide;  Rubino, Angelo
收藏  |  浏览/下载:13/0  |  提交时间:2019/04/09
Climate variability  Surface temperature  Numerical analysis  modeling  Spectral analysis  models  distribution  Time series  Climate variability  
Lightning activity in tropical cyclones and its relationship to dynamic and thermodynamic parameters over the northwest Pacific 期刊论文
ATMOSPHERIC RESEARCH, 2018, 213: 86-96
作者:  Wang, Fang;  Qie, Xiushu;  Wang, Dongfang;  Srivastava, Abhay
收藏  |  浏览/下载:24/0  |  提交时间:2019/04/09
Lightning rate  Tropical cyclone  Azimuthal distribution of lightning  Sea surface temperature  Vertical wind shear  
Season-dependent warming characteristics observed at 12 stations in South Korea over the recent 100 years 期刊论文
INTERNATIONAL JOURNAL OF CLIMATOLOGY, 2018, 38 (11) : 4092-4101
作者:  Choi, Woosuk;  Ho, Chang-Hoi;  Kim, Maeng-Ki;  Kim, Jinwon;  Yoo, Hee-Dong;  Jhun, Jong-Ghap;  Jeong, Jee-Hoon
收藏  |  浏览/下载:25/0  |  提交时间:2019/04/09
abrupt jump  change-point analysis  frequency distribution  surface air temperature  warming trend