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DOI | 10.1126/science.aah7111 |
CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells | |
Liu, S. John1,2; Horlbeck, Max A.3,4,5,6; Cho, Seung Woo9; Birk, Harjus S.1,2; Malatesta, Martina1,2; He, Daniel1,2; Attenello, Frank J.1,2; Villalta, Jacqueline E.3,4,5,6; Cho, Min Y.3,4,5,6; Chen, Yuwen3,4,5,6; Mandegar, Mohammad A.3; Olvera, Michael P.3; Gilbert, Luke A.3,4,5,6; Conklin, Bruce R.3,7,8; Chang, Howard Y.9; Weissman, Jonathan S.3,4,5,6; Lim, Daniel A.1,2,10 | |
2017-01-06 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6320 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The human genome produces thousands of long noncoding RNAs (lncRNAs)-transcripts >200 nucleotides long that do not encode proteins. Although critical roles in normal biology and disease have been revealed for a subset of lncRNAs, the function of the vast majority remains untested. We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth-modifying function exclusively in one cell type. We further found that lncRNA knockdown can perturb complex transcriptional networks in a cell type-specific manner. These data underscore the functional importance and cell type specificity of many lncRNAs. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391739900002 |
WOS关键词 | GENE-EXPRESSION ; STEM-CELLS ; DIFFERENTIATION ; TRANSCRIPTION ; EVOLUTION ; CANCER ; LNCRNA ; INTERFERENCE ; ACTIVATION ; ENHANCERS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195154 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA; 2.Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USA; 3.Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94143 USA; 4.Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA; 5.Univ Calif San Francisco, Calif Inst Quantitat Biomed Res, San Francisco, CA 94143 USA; 6.Univ Calif San Francisco, Ctr RNA Syst Biol, San Francisco, CA 94143 USA; 7.Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA; 8.Gladstone Inst Cardiovasc Dis, San Francisco, CA USA; 9.Stanford Univ, Ctr Personal Dynam Regulomes, Stanford, CA 94305 USA; 10.San Francisco VA Med Ctr, San Francisco, CA USA |
推荐引用方式 GB/T 7714 | Liu, S. John,Horlbeck, Max A.,Cho, Seung Woo,et al. CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells[J]. SCIENCE,2017,355(6320). |
APA | Liu, S. John.,Horlbeck, Max A..,Cho, Seung Woo.,Birk, Harjus S..,Malatesta, Martina.,...&Lim, Daniel A..(2017).CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells.SCIENCE,355(6320). |
MLA | Liu, S. John,et al."CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells".SCIENCE 355.6320(2017). |
条目包含的文件 | 条目无相关文件。 |
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