Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aan0096 |
Ghost cytometry | |
Ota, Sadao1,2,3; Horisaki, Ryoichi3,4; Kawamura, Yoko1,2; Ugawa, Masashi1; Sato, Issei1,2,3,5; Hashimoto, Kazuki2,6; Kamesawa, Ryosuke1,2; Setoyama, Kotaro1; Yamaguchi, Satoko2; Fujiu, Katsuhito2; Waki, Kayo2; Noji, Hiroyuki2,7 | |
2018-06-15 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6394页码:1246-1251 |
文章类型 | Article |
语种 | 英语 |
国家 | Japan |
英文摘要 | Ghost imaging is a technique used to produce an object's image without using a spatially resolving detector. Here we develop a technique we term "ghost cytometry," an image-free ultrafast fluorescence "imaging" cytometry based on a single-pixel detector. Spatial information obtained from the motion of cells relative to a static randomly patterned optical structure is compressively converted into signals that arrive sequentially at a single-pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random pattern allows us to computationally reconstruct cell morphology. More importantly, we show that applying machine-learning methods directly on the compressed waveforms without image reconstruction enables efficient image-free morphology-based cytometry. Despite a compact and inexpensive instrumentation, image-free ghost cytometry achieves accurate and high-throughput cell classification and selective sorting on the basis of cell morphology without a specific biomarker, both of which have been challenging to accomplish using conventional flow cytometers. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000435360400057 |
WOS关键词 | CIRCULATING TUMOR-CELLS ; FLOW-CYTOMETRY ; FLUORESCENCE MICROSCOPY ; BIOLOGY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198903 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Thinkcyte Inc, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138654, Japan; 2.Univ Tokyo, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138654, Japan; 3.Japan Sci & Technol Agcy, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan; 4.Osaka Univ, Grad Sch Informat Sci & Technol, Dept Informat & Phys Sci, 1-5 Yamadaoka, Suita, Osaka 5650871, Japan; 5.RIKEN, AIP, Chuo Ku, Nihonbashi 1-Chome Mitsui Bldg,1-4-1 Nihonbashi, Tokyo 1030027, Japan; 6.Japan Aerosp Explorat Agcy, 6-13-1 Osawa, Mitaka, Tokyo 1810015, Japan; 7.Govt Japan, Cabinet Off, ImPACT Program, Chiyoda Ku, Tokyo 1008914, Japan |
推荐引用方式 GB/T 7714 | Ota, Sadao,Horisaki, Ryoichi,Kawamura, Yoko,et al. Ghost cytometry[J]. SCIENCE,2018,360(6394):1246-1251. |
APA | Ota, Sadao.,Horisaki, Ryoichi.,Kawamura, Yoko.,Ugawa, Masashi.,Sato, Issei.,...&Noji, Hiroyuki.(2018).Ghost cytometry.SCIENCE,360(6394),1246-1251. |
MLA | Ota, Sadao,et al."Ghost cytometry".SCIENCE 360.6394(2018):1246-1251. |
条目包含的文件 | 条目无相关文件。 |
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