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DOI | 10.1126/science.aat7992 |
Semisynthetic sensor proteins enable metabolic assays at the point of care | |
Yu, Qiuliyang1,2; Xue, Lin1; Hiblot, Julien1; Griss, Rudolf2; Fabritz, Sebastian1; Roux, Clothilde3; Binz, Pierre-Alain3; Haas, Dorothea4; Okun, Juergen G.4; Johnsson, Kai1,2 | |
2018-09-14 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6407页码:1122-1125 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; Switzerland |
英文摘要 | Monitoring metabolites at the point of care could improve the diagnosis and management of numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins for use in paper-based metabolic assays. The metabolite is oxidized by nicotinamide adenine dinucleotide phosphate, and the sensor changes color in the presence of the reduced cofactor, enabling metabolite quantification with the use of a digital camera. The approach makes any metabolite that can be oxidized by the cofactor a candidate for quantitative point-of-care assays, as shown for phenylalanine, glucose, and glutamate. Phenylalanine blood levels of phenylketonuria patients were analyzed at the point of care within minutes with only 0.5 microliters of blood. Results were within 15% of those obtained with standard testing methods. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000444513300043 |
WOS关键词 | PHENYLKETONURIA ; DEHYDROGENASE ; INSULIN ; TARGETS ; GLUCOSE ; BLOOD ; STEP |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199579 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Med Res, Dept Biol Chem, D-69120 Heidelberg, Germany; 2.Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, NCCR Chem Biol, CH-1015 Lausanne, Switzerland; 3.Univ Hosp Lausanne, Clin Chem Lab, Serv Biomed, CH-1011 Lausanne, Switzerland; 4.Univ Childrens Hosp Heidelberg, Ctr Metab Dis, Metab Lab, D-69120 Heidelberg, Germany |
推荐引用方式 GB/T 7714 | Yu, Qiuliyang,Xue, Lin,Hiblot, Julien,et al. Semisynthetic sensor proteins enable metabolic assays at the point of care[J]. SCIENCE,2018,361(6407):1122-1125. |
APA | Yu, Qiuliyang.,Xue, Lin.,Hiblot, Julien.,Griss, Rudolf.,Fabritz, Sebastian.,...&Johnsson, Kai.(2018).Semisynthetic sensor proteins enable metabolic assays at the point of care.SCIENCE,361(6407),1122-1125. |
MLA | Yu, Qiuliyang,et al."Semisynthetic sensor proteins enable metabolic assays at the point of care".SCIENCE 361.6407(2018):1122-1125. |
条目包含的文件 | 条目无相关文件。 |
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