GSTDTAP  > 地球科学
DOI10.1126/science.aau5324
Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region
Moffitt, Jeffrey R.1,2,3,4; Bambah-Mukku, Dhananjay1,4,5; Eichhorn, Stephen W.1,2,3,4; Vaughn, Eric1,4,5; Shekhar, Karthik6; Perez, Julio D.1,4,5; Rubinstein, Nimrod D.1,4,5; Hao, Junjie1,2,3,4; Regev, Aviv1,6,7; Dulac, Catherine1,4,5; Zhuang, Xiaowei1,2,3,4
2018-11-16
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
出版年2018
卷号362期号:6416页码:792-+
文章类型Article
语种英语
国家USA
英文摘要

The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei-including the molecular identity, spatial organization, and function of distinct cell types-is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled similar to 1 million cells, identified similar to 70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000450488500043
WOS关键词GENE-EXPRESSION ; STRIA TERMINALIS ; BED NUCLEUS ; NEURONS ; CIRCUITS ; AREA ; IDENTIFICATION ; SPECIFICATION ; ORGANIZATION ; REPRODUCTION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/200075
专题地球科学
资源环境科学
气候变化
作者单位1.Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA;
2.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA;
3.Harvard Univ, Dept Phys, Cambridge, MA 02138 USA;
4.Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA;
5.Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA;
6.Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02139 USA;
7.MIT, Koch Inst Integrat Canc Biol, Dept Biol, Cambridge, MA 02139 USA
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GB/T 7714
Moffitt, Jeffrey R.,Bambah-Mukku, Dhananjay,Eichhorn, Stephen W.,et al. Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region[J]. SCIENCE,2018,362(6416):792-+.
APA Moffitt, Jeffrey R..,Bambah-Mukku, Dhananjay.,Eichhorn, Stephen W..,Vaughn, Eric.,Shekhar, Karthik.,...&Zhuang, Xiaowei.(2018).Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region.SCIENCE,362(6416),792-+.
MLA Moffitt, Jeffrey R.,et al."Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region".SCIENCE 362.6416(2018):792-+.
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