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DOI | 10.1126/science.aau5324 |
Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region | |
Moffitt, Jeffrey R.1,2,3,4; Bambah-Mukku, Dhananjay1,4,5; Eichhorn, Stephen W.1,2,3,4; Vaughn, Eric1,4,5; Shekhar, Karthik6; Perez, Julio D.1,4,5; Rubinstein, Nimrod D.1,4,5; Hao, Junjie1,2,3,4; Regev, Aviv1,6,7; Dulac, Catherine1,4,5; Zhuang, Xiaowei1,2,3,4 | |
2018-11-16 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6416页码:792-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei-including the molecular identity, spatial organization, and function of distinct cell types-is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled similar to 1 million cells, identified similar to 70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450488500043 |
WOS关键词 | GENE-EXPRESSION ; STRIA TERMINALIS ; BED NUCLEUS ; NEURONS ; CIRCUITS ; AREA ; IDENTIFICATION ; SPECIFICATION ; ORGANIZATION ; REPRODUCTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/200075 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA; 2.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA; 3.Harvard Univ, Dept Phys, Cambridge, MA 02138 USA; 4.Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA; 5.Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA; 6.Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02139 USA; 7.MIT, Koch Inst Integrat Canc Biol, Dept Biol, Cambridge, MA 02139 USA |
推荐引用方式 GB/T 7714 | Moffitt, Jeffrey R.,Bambah-Mukku, Dhananjay,Eichhorn, Stephen W.,et al. Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region[J]. SCIENCE,2018,362(6416):792-+. |
APA | Moffitt, Jeffrey R..,Bambah-Mukku, Dhananjay.,Eichhorn, Stephen W..,Vaughn, Eric.,Shekhar, Karthik.,...&Zhuang, Xiaowei.(2018).Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region.SCIENCE,362(6416),792-+. |
MLA | Moffitt, Jeffrey R.,et al."Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region".SCIENCE 362.6416(2018):792-+. |
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