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DOI | 10.1038/s41467-017-02509-w |
Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila | |
Wilson, Jason S.1; Churchill-Angus, Alicia M.1; Davies, Simon P.1,2,3; Sedelnikova, Svetlana E.1; Tzokov, Svetomir B.1; Rafferty, John B.1; Bullough, Per A.1; Bisson, Claudine1,4; Baker, Patrick J.1 | |
2019-07-01 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | England |
英文摘要 | The alpha helical CytolysinA family of pore forming toxins (alpha-PFT) contains single, two, and three component members. Structures of the single component Eschericia coli ClyA and the two component Yersinia enterolytica YaxAB show both undergo conformational changes from soluble to pore forms, and oligomerization to produce the active pore. Here we identify tripartite alpha-PFTs in pathogenic Gram negative bacteria, including Aeromonas hydrophila (AhlABC). We show that the AhlABC toxin requires all three components for maximal cell lysis. We present structures of pore components which describe a bi-fold hinge mechanism for soluble to pore transition in AhlB and a contrasting tetrameric assembly employed by soluble AhlC to hide their hydrophobic membrane associated residues. We propose a model of pore assembly where the AhlC tetramer dissociates, binds a single membrane leaflet, recruits AhlB promoting soluble to pore transition, prior to AhlA binding to form the active hydrophilic lined pore. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000473296000007 |
WOS关键词 | RAY CRYSTAL-STRUCTURE ; HEMOLYSIN-E HLYE ; BL ; BINDING ; CLYA ; COMPONENTS ; CHANNEL ; SYSTEM ; SHEA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203608 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Sheffield, Dept Mol Biol & Biotechnol, Western Bank, Sheffield S10 2TN, S Yorkshire, England; 2.Univ Leeds, Sch Biomed Sci, Fac Biol Sci, Leeds LS2 9JT, W Yorkshire, England; 3.Univ Leeds, Astbury Ctr Struct & Mol Biol, Leeds LS2 9JT, W Yorkshire, England; 4.Birkbeck Univ London, ISMB, Dept Biol Sci, Malet St, London WC1E 7HX, England |
推荐引用方式 GB/T 7714 | Wilson, Jason S.,Churchill-Angus, Alicia M.,Davies, Simon P.,et al. Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Wilson, Jason S..,Churchill-Angus, Alicia M..,Davies, Simon P..,Sedelnikova, Svetlana E..,Tzokov, Svetomir B..,...&Baker, Patrick J..(2019).Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila.NATURE COMMUNICATIONS,10. |
MLA | Wilson, Jason S.,et al."Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila".NATURE COMMUNICATIONS 10(2019). |
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