GSTDTAP  > 资源环境科学
DOI10.1038/s41467-017-02509-w
Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila
Wilson, Jason S.1; Churchill-Angus, Alicia M.1; Davies, Simon P.1,2,3; Sedelnikova, Svetlana E.1; Tzokov, Svetomir B.1; Rafferty, John B.1; Bullough, Per A.1; Bisson, Claudine1,4; Baker, Patrick J.1
2019-07-01
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2019
卷号10
文章类型Article
语种英语
国家England
英文摘要

The alpha helical CytolysinA family of pore forming toxins (alpha-PFT) contains single, two, and three component members. Structures of the single component Eschericia coli ClyA and the two component Yersinia enterolytica YaxAB show both undergo conformational changes from soluble to pore forms, and oligomerization to produce the active pore. Here we identify tripartite alpha-PFTs in pathogenic Gram negative bacteria, including Aeromonas hydrophila (AhlABC). We show that the AhlABC toxin requires all three components for maximal cell lysis. We present structures of pore components which describe a bi-fold hinge mechanism for soluble to pore transition in AhlB and a contrasting tetrameric assembly employed by soluble AhlC to hide their hydrophobic membrane associated residues. We propose a model of pore assembly where the AhlC tetramer dissociates, binds a single membrane leaflet, recruits AhlB promoting soluble to pore transition, prior to AhlA binding to form the active hydrophilic lined pore.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000473296000007
WOS关键词RAY CRYSTAL-STRUCTURE ; HEMOLYSIN-E HLYE ; BL ; BINDING ; CLYA ; COMPONENTS ; CHANNEL ; SYSTEM ; SHEA
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
URL查看原文
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/203608
专题资源环境科学
作者单位1.Univ Sheffield, Dept Mol Biol & Biotechnol, Western Bank, Sheffield S10 2TN, S Yorkshire, England;
2.Univ Leeds, Sch Biomed Sci, Fac Biol Sci, Leeds LS2 9JT, W Yorkshire, England;
3.Univ Leeds, Astbury Ctr Struct & Mol Biol, Leeds LS2 9JT, W Yorkshire, England;
4.Birkbeck Univ London, ISMB, Dept Biol Sci, Malet St, London WC1E 7HX, England
推荐引用方式
GB/T 7714
Wilson, Jason S.,Churchill-Angus, Alicia M.,Davies, Simon P.,et al. Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila[J]. NATURE COMMUNICATIONS,2019,10.
APA Wilson, Jason S..,Churchill-Angus, Alicia M..,Davies, Simon P..,Sedelnikova, Svetlana E..,Tzokov, Svetomir B..,...&Baker, Patrick J..(2019).Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila.NATURE COMMUNICATIONS,10.
MLA Wilson, Jason S.,et al."Identification and structural analysis of the tripartite alpha-pore forming toxin of Aeromonas hydrophila".NATURE COMMUNICATIONS 10(2019).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wilson, Jason S.]的文章
[Churchill-Angus, Alicia M.]的文章
[Davies, Simon P.]的文章
百度学术
百度学术中相似的文章
[Wilson, Jason S.]的文章
[Churchill-Angus, Alicia M.]的文章
[Davies, Simon P.]的文章
必应学术
必应学术中相似的文章
[Wilson, Jason S.]的文章
[Churchill-Angus, Alicia M.]的文章
[Davies, Simon P.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。