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DOI | 10.1038/s41467-017-02274-w |
Pentatricopeptide repeat poly(A) binding protein KPAF4 stabilizes mitochondrial mRNAs in Trypanosoma brucei | |
Mesitov, Mikhail V.1; Yu, Tian1,2; Suematsu, Takuma1; Sement, Francois M.1; Zhang, Liye3; Yu, Clinton4; Huang, Lan4; Aphasizheva, Inna1 | |
2019-01-11 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Peoples R China |
英文摘要 | In Trypanosoma brucei, most mitochondrial mRNAs undergo editing, and 3' adenylation and uridylation. The internal sequence changes and terminal extensions are coordinated: pre-editing addition of the short (A) tail protects the edited transcript against 3'-5' degradation, while post-editing A/U-tailing renders mRNA competent for translation. Participation of a poly(A) binding protein (PABP) in coupling of editing and 3' modification processes has been inferred, but its identity and mechanism of action remained elusive. We report identification of KPAF4, a pentatricopeptide repeat-containing PABP which sequesters the A-tail and impedes mRNA degradation. Conversely, KPAF4 inhibits uridylation of A-tailed transcripts and, therefore, premature A/U-tailing of partially-edited mRNAs. This quality check point likely prevents translation of incompletely edited mRNAs. We also find that RNA editing substrate binding complex (RESC) mediates the interaction between the 5' end-bound pyrophosphohydrolase MERS1 and 3' end-associated KPAF4 to enable mRNA circularization. This event appears to be critical for edited mRNA stability. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000455473700014 |
WOS关键词 | INDUCIBLE EXPRESSION SYSTEM ; GUIDE-RNA ; RIBOSOMAL-RNA ; COMPLEX ; RECOGNITION ; POLYADENYLATION ; TUTASE ; ROLES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203639 |
专题 | 资源环境科学 |
作者单位 | 1.Boston Univ, Dept Mol & Cell Biol, Med Campus, Boston, MA 02118 USA; 2.Boston Univ, Bioinformat Program, Boston, MA 02215 USA; 3.ShanghaiTechUniversity, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 4.Univ Calif Irvine, Sch Med, Dept Physiol & Biophys, Irvine, CA 92697 USA |
推荐引用方式 GB/T 7714 | Mesitov, Mikhail V.,Yu, Tian,Suematsu, Takuma,et al. Pentatricopeptide repeat poly(A) binding protein KPAF4 stabilizes mitochondrial mRNAs in Trypanosoma brucei[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Mesitov, Mikhail V..,Yu, Tian.,Suematsu, Takuma.,Sement, Francois M..,Zhang, Liye.,...&Aphasizheva, Inna.(2019).Pentatricopeptide repeat poly(A) binding protein KPAF4 stabilizes mitochondrial mRNAs in Trypanosoma brucei.NATURE COMMUNICATIONS,10. |
MLA | Mesitov, Mikhail V.,et al."Pentatricopeptide repeat poly(A) binding protein KPAF4 stabilizes mitochondrial mRNAs in Trypanosoma brucei".NATURE COMMUNICATIONS 10(2019). |
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