Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-018-04491-3 |
Megahertz data collection from protein microcrystals at an X-ray free-electron laser | |
Grunbein, Marie Luise1; Bielecki, Johan2; Gorel, Alexander1; Stricker, Miriam1; Bean, Richard2; Cammarata, Marco3; Doerner, Katerina2; Froehlich, Lars4; Hartmann, Elisabeth1; Hauf, Steffen2; Hilpert, Mario1,2; Kim, Yoonhee2; Kloos, Marco1; Letrun, Romain2; Messerschmidt, Marc2,5; Mills, Grant2,6; Kovacs, Gabriela Nass1; Ramilli, Marco2; Roome, Christopher M.1; Sato, Tokushi2,7; Scholz, Matthias4; Sliwa, Michel8; Sztuk-Dambietz, Jolanta2; Weik, Martin9; Weinhausen, Britta2; Al-Qudami, Nasser2; Boukhelef, Djelloul2; Brockhauser, Sandor2,10; Ehsan, Wajid2; Emons, Moritz2; Esenov, Sergey2; Fangohr, Hans2; Kaukher, Alexander2; Kluyver, Thomas2; Lederer, Max2; Maia, Luis2; Manetti, Maurizio2; Michelat, Thomas2; Muennich, Astrid2; Pallas, Florent2; Palmer, Guido2; Previtali, Gianpietro2; Raab, Natascha2; Silenzi, Alessandro2; Szuba, Janusz2; Venkatesan, Sandhya2; Wrona, Krzysztof2; Zhu, Jun2; Doak, R. Bruce1; Shoeman, Robert L.1; Foucar, Lutz1; Colletier, Jacques-Philippe9; Mancuso, Adrian P.2; Barends, Thomas R. M.1; Stan, Claudiu A.11; Schlichting, Ilme1 | |
2018-08-28 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; France; USA; Australia; Hungary |
英文摘要 | X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10-120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a similar to 15 mu m XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000442909300011 |
WOS关键词 | ANGSTROM RESOLUTION ; CRYSTAL-STRUCTURE ; CONCANAVALIN-A ; CRYSTALLIZATION ; DIFFRACTION ; SYSTEM ; UREASE ; MODEL |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203825 |
专题 | 资源环境科学 |
作者单位 | 1.Max Planck Inst Med Res, Jahnstr 29, D-69120 Heidelberg, Germany; 2.European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany; 3.Univ Rennes 1, Dept Phys, UMR 625, UBL, F-35042 Rennes, France; 4.Deutsch Elektronensynchrotron DESY, Notkestr 85, D-22607 Hamburg, Germany; 5.BioXFEL STC, 700 Ellicott St, Buffalo, NY 14203 USA; 6.La Trobe Univ, La Trobe Inst Mol Sci, ARC Ctr Excellence Adv Mol Imaging, Melbourne, Vic 3086, Australia; 7.Deutsch Elektronensynchrotron, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany; 8.Univ Lille, Lab Spectrochim Infrarouge & Raman, CNRS, UMR 8516, F-59000 Lille, France; 9.Univ Grenoble Alpes, CNRS, Inst Biol Struct, CEA, F-38044 Grenoble, France; 10.Hungarian Acad Sci, Biol Res Ctr BRC, Temesvari Krt 62, H-6726 Szeged, Hungary; 11.Rutgers Univ Newark, Dept Phys, 101 Warren St, Newark, NJ 07102 USA |
推荐引用方式 GB/T 7714 | Grunbein, Marie Luise,Bielecki, Johan,Gorel, Alexander,et al. Megahertz data collection from protein microcrystals at an X-ray free-electron laser[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Grunbein, Marie Luise.,Bielecki, Johan.,Gorel, Alexander.,Stricker, Miriam.,Bean, Richard.,...&Schlichting, Ilme.(2018).Megahertz data collection from protein microcrystals at an X-ray free-electron laser.NATURE COMMUNICATIONS,9. |
MLA | Grunbein, Marie Luise,et al."Megahertz data collection from protein microcrystals at an X-ray free-electron laser".NATURE COMMUNICATIONS 9(2018). |
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