GSTDTAP  > 资源环境科学
DOI10.1038/s41467-018-05488-8
Allosteric modulation of protein-protein interactions by individual lipid binding events
Cong, Xiao1,3; Liu, Yang1; Liu, Wen1; Liang, Xiaowen1; Laganowsky, Arthur1,2
2017-12-19
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2017
卷号8
文章类型Article
语种英语
国家USA
英文摘要

The diverse lipid environment of the biological membrane can modulate the structure and function of membrane proteins. However, little is known about the role that lipids play in modulating protein-protein interactions. Here we employed native mass spectrometry (MS) to determine how individual lipid-binding events to the ammonia channel (AmtB) modulate its interaction with the regulatory protein, GlnK. The thermodynamic signature of AmtB-GlnK in the absence of lipids indicates conformational dynamics. A small number of lipids bound to AmtB is sufficient to modulate the interaction with GlnK, and lipids with different headgroups display a range of allosteric modulation. We also find that lipid chain length and stereochemistry can affect the degree of allosteric modulation, indicating an unforeseen selectivity of membrane proteins toward the chemistry of lipid tails. These results demonstrate that individual lipid-binding events can allosterically modulate the interactions of integral membrane and soluble proteins.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000418336800008
WOS关键词IONIZATION MASS-SPECTROMETRY ; GLNK COMPLEX-FORMATION ; ESCHERICHIA-COLI ; HEAT-CAPACITY ; MEMBRANE-PROTEINS ; LIGAND INTERACTIONS ; AMMONIA CHANNEL ; ION CHANNELS ; GAS-PHASE ; REVEALS
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/203947
专题资源环境科学
作者单位1.Texas A&M Hlth Sci Ctr, Inst Biosci & Technol, Houston, TX 77030 USA;
2.Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA;
3.Wolfe Labs Inc, 19 Presidential Way, Woburn, MA 01801 USA
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GB/T 7714
Cong, Xiao,Liu, Yang,Liu, Wen,et al. Allosteric modulation of protein-protein interactions by individual lipid binding events[J]. NATURE COMMUNICATIONS,2017,8.
APA Cong, Xiao,Liu, Yang,Liu, Wen,Liang, Xiaowen,&Laganowsky, Arthur.(2017).Allosteric modulation of protein-protein interactions by individual lipid binding events.NATURE COMMUNICATIONS,8.
MLA Cong, Xiao,et al."Allosteric modulation of protein-protein interactions by individual lipid binding events".NATURE COMMUNICATIONS 8(2017).
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