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DOI | 10.1038/s41467-018-05488-8 |
Allosteric modulation of protein-protein interactions by individual lipid binding events | |
Cong, Xiao1,3; Liu, Yang1; Liu, Wen1; Liang, Xiaowen1; Laganowsky, Arthur1,2 | |
2017-12-19 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The diverse lipid environment of the biological membrane can modulate the structure and function of membrane proteins. However, little is known about the role that lipids play in modulating protein-protein interactions. Here we employed native mass spectrometry (MS) to determine how individual lipid-binding events to the ammonia channel (AmtB) modulate its interaction with the regulatory protein, GlnK. The thermodynamic signature of AmtB-GlnK in the absence of lipids indicates conformational dynamics. A small number of lipids bound to AmtB is sufficient to modulate the interaction with GlnK, and lipids with different headgroups display a range of allosteric modulation. We also find that lipid chain length and stereochemistry can affect the degree of allosteric modulation, indicating an unforeseen selectivity of membrane proteins toward the chemistry of lipid tails. These results demonstrate that individual lipid-binding events can allosterically modulate the interactions of integral membrane and soluble proteins. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000418336800008 |
WOS关键词 | IONIZATION MASS-SPECTROMETRY ; GLNK COMPLEX-FORMATION ; ESCHERICHIA-COLI ; HEAT-CAPACITY ; MEMBRANE-PROTEINS ; LIGAND INTERACTIONS ; AMMONIA CHANNEL ; ION CHANNELS ; GAS-PHASE ; REVEALS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203947 |
专题 | 资源环境科学 |
作者单位 | 1.Texas A&M Hlth Sci Ctr, Inst Biosci & Technol, Houston, TX 77030 USA; 2.Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA; 3.Wolfe Labs Inc, 19 Presidential Way, Woburn, MA 01801 USA |
推荐引用方式 GB/T 7714 | Cong, Xiao,Liu, Yang,Liu, Wen,et al. Allosteric modulation of protein-protein interactions by individual lipid binding events[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Cong, Xiao,Liu, Yang,Liu, Wen,Liang, Xiaowen,&Laganowsky, Arthur.(2017).Allosteric modulation of protein-protein interactions by individual lipid binding events.NATURE COMMUNICATIONS,8. |
MLA | Cong, Xiao,et al."Allosteric modulation of protein-protein interactions by individual lipid binding events".NATURE COMMUNICATIONS 8(2017). |
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