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DOI | 10.1038/s41467-018-07996-z |
Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments | |
Richter, David1; Moraga, Ignacio2,3,4; Winkelmann, Hauke1; Birkholz, Oliver1; Wilmes, Stephan1; Schulte, Markos5; Kraich, Michael5; Kenneweg, Hella1; Beutel, Oliver1; Selenschik, Philipp1; Paterok, Dirk1; Gavutis, Martynas1; Schmidt, Thomas6; Garcia, K. Christopher2,3,4; Mueller, Thomas D.5; Piehler, Jacob1 | |
2019-01-16 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; USA; Netherlands |
英文摘要 | The spatiotemporal organization of cytokine receptors in the plasma membrane is still debated with models ranging from ligand-independent receptor pre-dimerization to ligand-induced receptor dimerization occurring only after receptor uptake into endosomes. Here, we explore the molecular and cellular determinants governing the assembly of the type II interleukin-4 receptor, taking advantage of various agonists binding the receptor subunits with different affinities and rate constants. Quantitative kinetic studies using artificial membranes confirm that receptor dimerization is governed by the two-dimensional ligand-receptor interactions and identify a critical role of the transmembrane domain in receptor dimerization. Single molecule localization microscopy at physiological cell surface expression levels, however, reveals efficient ligand-induced receptor dimerization by all ligands, largely independent of receptor binding affinities, in line with the similar STAT6 activation potencies observed for all IL-4 variants. Detailed spatiotemporal analyses suggest that kinetic trapping of receptor dimers in actin-dependent microcompartments sustains robust receptor dimerization and signalling. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000405465400001 |
WOS关键词 | POLYMER-SUPPORTED MEMBRANES ; SINGLE-MOLECULE TECHNIQUES ; RESONANCE ENERGY-TRANSFER ; GROWTH-HORMONE RECEPTOR ; CELL-SURFACE ; ERYTHROPOIETIN RECEPTOR ; LIVING CELLS ; T-CELLS ; TRANSMEMBRANE PROTEINS ; MODEL MEMBRANES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204170 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Osnabruck, Dept Biol, Barbarastr 11, D-49076 Osnabruck, Germany; 2.Stanford Univ, Sch Med, Howard Hughes Med Inst, 279 Campus Dr, Stanford, CA 94305 USA; 3.Stanford Univ, Sch Med, Dept Mol & Cellular Physiol, 279 Campus Dr, Stanford, CA 94305 USA; 4.Stanford Univ, Sch Med, Dept Biol Struct, 279 Campus Dr, Stanford, CA 94305 USA; 5.Univ Wurzburg, Julius von Sachs Inst, Dept Mol Plant Physiol & Biophys, Julius von Sachs Pl 2, D-97082 Wurzburg, Germany; 6.Leiden Univ, Leiden Inst Phys, Phys Life Proc, Niels Bohrweg 2, NL-2333 AC Leiden, Netherlands |
推荐引用方式 GB/T 7714 | Richter, David,Moraga, Ignacio,Winkelmann, Hauke,et al. Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Richter, David.,Moraga, Ignacio.,Winkelmann, Hauke.,Birkholz, Oliver.,Wilmes, Stephan.,...&Piehler, Jacob.(2019).Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments.NATURE COMMUNICATIONS,8. |
MLA | Richter, David,et al."Ligand-induced type II interleukin-4 receptor dimers are sustained by rapid re-association within plasma membrane microcompartments".NATURE COMMUNICATIONS 8(2019). |
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