Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-10028-z |
The effects of death and post-mortem cold ischemia on human tissue transcriptomes | |
Ferreira, Pedro G.1,2; Munoz-Aguirre, Manuel3,4,5,6; Reverter, Ferran4,5,7; Sa Godinho, Caio P.8; Sousa, Abel1,2,9; Amadoz, Alicia10; Sodaei, Reza3,4,5; Hidalgo, Marta R.11; Pervouchine, Dmitri3,4,5,12; Carbonell-Caballero, Jose13; Nurtdinov, Ramil3,4,5; Breschi, Alessandra3,5; Amador, Raziel3,5; Oliveira, Patricia1,2; Cubuk, Cankut11; Curado, Joao3,5; Aguet, Francois14; Oliveira, Carla1,2; Dopazo, Joaquin10,11,15,16; Sammeth, Michael8; Ardlie, Kristin G.14; Guigo, Roderic3,4,5 | |
2019-05-15 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Portugal; Spain; Brazil; England; Russia; USA |
英文摘要 | Post-mortem tissues samples are a key resource for investigating patterns of gene expression. However, the processes triggered by death and the post-mortem interval (PMI) can significantly alter physiologically normal RNA levels. We investigate the impact of PMI on gene expression using data from multiple tissues of post-mortem donors obtained from the GTEx project. We find that many genes change expression over relatively short PMIs in a tissue-specific manner, but this potentially confounding effect in a biological analysis can be minimized by taking into account appropriate covariates. By comparing ante-and postmortem blood samples, we identify the cascade of transcriptional events triggered by death of the organism. These events do not appear to simply reflect stochastic variation resulting from mRNA degradation, but active and ongoing regulation of transcription. Finally, we develop a model to predict the time since death from the analysis of the transcriptome of a few readily accessible tissues. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000424872300001 |
WOS关键词 | RNA-SEQ DATA ; DIFFERENTIAL EXPRESSION ANALYSIS ; QUANTIFYING MESSENGER-RNA ; GENE-EXPRESSION ; GTEX PROJECT ; HUMAN BRAIN ; INTEGRITY ; DEGRADATION ; PACKAGE ; STABILITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204367 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Porto, Inst Invest & Inovacao Saude, Rua Alfredo Allen 208, P-4200135 Oporto, Portugal; 2.Univ Porto, Inst Mol Pathol & Immunol, Rua Dr Roberto Frias S-N, P-4200625 Oporto, Portugal; 3.Barcelona Inst Sci & Technol, Ctr Genom Regulat CRG, Dr Aiguader 88, E-08003 Barcelona, Catalonia, Spain; 4.UPF, E-08003 Barcelona, Catalonia, Spain; 5.Inst Hosp Mar Invest Mediques IMIM, E-08003 Barcelona, Catalonia, Spain; 6.Univ Politecn Cataluna, Dept Estadist & Invest Operat, E-08034 Barcelona, Catalonia, Spain; 7.Univ Barcelona, E-08028 Barcelona, Catalonia, Spain; 8.Fed Univ Rio De Janeiro UFRJ, IBCCF, BR-21941902 Rio De Janeiro, Brazil; 9.EBI, EMBL, Wellcome Genome Campus, Cambridge CB10 1SD, England; 10.Igenomix SA, Dept Bioinformat, Valencia 46980, Spain; 11.Hosp Virgen Rocio, Clin Bioinformat Area, FPS, Seville 41013, Spain; 12.Skolkovo Inst Sci & Technol, 100 Novaya St, Skolkovo 143025, Moscow Region, Russia; 13.PRBB, Barcelona Inst Sci & Technol, Gene Regulat Stem Cells & Canc Program, Chromatin & Gene Express Lab,CRG, Barcelona 08003, Spain; 14.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA; 15.Hosp Virgen Rocio, Funct Genom Node INB, FPS, Seville 41013, Spain; 16.Hosp Virgen Rocio, FPS, CIBERER, Bioinformat Rare Dis BiER, Seville 41013, Spain |
推荐引用方式 GB/T 7714 | Ferreira, Pedro G.,Munoz-Aguirre, Manuel,Reverter, Ferran,et al. The effects of death and post-mortem cold ischemia on human tissue transcriptomes[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Ferreira, Pedro G..,Munoz-Aguirre, Manuel.,Reverter, Ferran.,Sa Godinho, Caio P..,Sousa, Abel.,...&Guigo, Roderic.(2019).The effects of death and post-mortem cold ischemia on human tissue transcriptomes.NATURE COMMUNICATIONS,9. |
MLA | Ferreira, Pedro G.,et al."The effects of death and post-mortem cold ischemia on human tissue transcriptomes".NATURE COMMUNICATIONS 9(2019). |
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