Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1073/pnas.1619871114 |
Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions | |
Krause, Sascha M. B.1,2; Johnson, Timothy2; Karunaratne, Yasodara Samadhi2; Fu, Yanfen1; Beck, David A. C.1,3; Chistoserdova, Ludmila1; Lidstrom, Mary E.1,2 | |
2017-01-10 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
出版年 | 2017 |
卷号 | 114期号:2页码:358-363 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The utilization of methane, a potent greenhouse gas, is an important component of local and global carbon cycles that is characterized by tight linkages between methane-utilizing (methanotrophic) and nonmethanotrophic bacteria. It has been suggested that the methanotroph sustains these nonmethanotrophs by cross-feeding, because subsequent products of the methane oxidation pathway, such as methanol, represent alternative carbon sources. We established cocultures in a microcosm model system to determine the mechanism and substrate that underlay the observed cross-feeding in the environment. Lanthanum, a rare earth element, was applied because of its increasing importance in methylotrophy. We used co-occurring strains isolated from Lake Washington sediment that are involved in methane utilization: a methanotroph and two nonmethanotrophic methylotrophs. Gene-expression profiles and mutant analyses suggest that methanol is the dominant carbon and energy source the methanotroph provides to support growth of the nonmethanotrophs. However, in the presence of the nonmethanotroph, gene expression of the dominant methanol dehydrogenase (MDH) shifts from the lanthanide-dependent MDH (XoxF)-type, to the calcium-dependent MDH (MxaF)-type. Correspondingly, methanol is released into the medium only when the methanotroph expresses the MxaF-type MDH. These results suggest a cross-feeding mechanism in which the nonmethanotrophic partner induces a change in expression of methanotrophMDHs, resulting in release of methanol for its growth. This partner-induced change in gene expression that benefits the partner is a paradigm for microbial interactions that cannot be observed in studies of pure cultures, underscoring the importance of synthetic microbial community approaches to understand environmental microbiomes. |
英文关键词 | synthetic ecology eco-physiology flow cytometry Methylobacter metabolic transformation |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391439300052 |
WOS关键词 | DIFFERENTIAL EXPRESSION ANALYSIS ; METHANOTROPHIC BACTERIA ; METHYLOTENERA-MOBILIS ; TROPHIC INTERACTIONS ; NATURAL HABITATS ; LAKE SEDIMENT ; RIBOSOMAL-RNA ; RICE FIELD ; DIVERSITY ; SOIL |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204713 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Washington, Dept Chem Engn, Seattle, WA 98195 USA; 2.Univ Washington, Dept Microbiol, Seattle, WA 98195 USA; 3.Univ Washington, ESci Inst, Seattle, WA 98195 USA |
推荐引用方式 GB/T 7714 | Krause, Sascha M. B.,Johnson, Timothy,Karunaratne, Yasodara Samadhi,et al. Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(2):358-363. |
APA | Krause, Sascha M. B..,Johnson, Timothy.,Karunaratne, Yasodara Samadhi.,Fu, Yanfen.,Beck, David A. C..,...&Lidstrom, Mary E..(2017).Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(2),358-363. |
MLA | Krause, Sascha M. B.,et al."Lanthanide-dependent cross-feeding of methane-derived carbon is linked by microbial community interactions".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.2(2017):358-363. |
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