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DOI | 10.1073/pnas.1909706116 |
Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii | |
Mukherjee, Ananya1; Lau, Chun Sing2; Walker, Charlotte E.2; Rai, Ashwani K.1; Prejean, Camille I.1; Yates, Gary2; Emrich-Mills, Thomas2; Lemoine, Spencer G.1; Vinyard, David J.1; Mackinder, Luke C. M.2; Moroney, James V.1 | |
2019 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
出版年 | 2019 |
卷号 | 116期号:34页码:16915-16920 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England |
英文摘要 | The green alga Chlamydomonas reinhardtii possesses a CO2 concentrating mechanism (CCM) that helps in successful acclimation to low CO2 conditions. Current models of the CCM postulate that a series of ion transporters bring HCO3- from outside the cell to the thylakoid lumen, where the carbonic anhydrase 3 (CAH3) dehydrates accumulated HCO3- to CO2, raising the CO2 concentration for Ribulose bisphosphate carboxylase/oxygenase (Rubisco). Previously, HCO3- transporters have been identified at both the plasma membrane and the chloroplast envelope, but the transporter thought to be on the thylakoid membrane has not been identified. Three paralogous genes (BST1, BST2, and BST3) belonging to the bestrophin family have been found to be up-regulated in low CO2 conditions, and their expression is controlled by CIA5, a transcription factor that controls many CCM genes. YFP fusions demonstrate that all 3 proteins are located on the thylakoid membrane, and interactome studies indicate that they might associate with chloroplast CCM components. A single mutant defective in BST3 has near-normal growth on low CO2, indicating that the 3 bestrophin-like proteins may have redundant functions. Therefore, an RNA interference (RNAi) approach was adopted to reduce the expression of all 3 genes at once. RNAi mutants with reduced expression of BST1-3 were unable to grow at low CO2 concentrations, exhibited a reduced affinity to inorganic carbon (Ci) compared with the wild-type cells, and showed reduced Ci uptake. We propose that these bestrophin-like proteins are essential components of the CCM that deliver HCO3- accumulated in the chloroplast stroma to CAH3 inside the thylakoid lumen. |
英文关键词 | Chlamydomonas CO2 concentrating mechanism bicarbonate transport photosynthesis chloroplast thylakoid |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000481935500043 |
WOS关键词 | CARBON-DIOXIDE ; CO2-CONCENTRATING MECHANISM ; MUTANT ; GENE ; LCIB ; PHOTOSYNTHESIS ; REVEALS ; PHOTORESPIRATION ; EXPRESSION ; DEFICIENT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/205162 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA; 2.Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England |
推荐引用方式 GB/T 7714 | Mukherjee, Ananya,Lau, Chun Sing,Walker, Charlotte E.,et al. Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(34):16915-16920. |
APA | Mukherjee, Ananya.,Lau, Chun Sing.,Walker, Charlotte E..,Rai, Ashwani K..,Prejean, Camille I..,...&Moroney, James V..(2019).Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(34),16915-16920. |
MLA | Mukherjee, Ananya,et al."Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.34(2019):16915-16920. |
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