Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP3953 |
Evaluation of Bisphenol A (BPA) Exposures on Prostate Stem Cell Homeostasis and Prostate Cancer Risk in the NCTR-Sprague-Dawley Rat: An NIEHS/FDA CLARITY-BPA Consortium Study | |
Prins, Gail S.1,2,3,4; Hu, Wen-Yang1,3; Xie, Lishi1,3; Shi, Guang-Bin1; Hu, Dan-Ping1; Birch, Lynn1; Bosland, Maarten C.3,4 | |
2018-11-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES |
ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2018 |
卷号 | 126期号:11 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | BACKGROUND: Previous work determined that early life exposure to low-dose Bisphenol A (BPA) increased rat prostate cancer risk with aging. Herein, we report on prostate-specific results from CLARITY-BPA (Consortium Linking Academic and Regulatory Insights on BPA Toxicity), which aims to resolve uncertainties regarding BPA toxicity. OBJECTIVES: We sought to a) reassess whether a range of BPA exposures drives prostate pathology and/or alters prostatic susceptibility to hormonal carcinogenesis, and b) test whether chronic low-dose BPA targets prostate epithelial stem and progenitor cells. METHODS: Sprague-Dawley rats were gavaged daily with vehicle, ethinyl estradiol (EE) or 2.5-25,000 mu g BPA/kg-BW during development or chronically, and prostate pathology was assessed at one year. One developmentally exposed cohort was given testosterone plus estradiol (T+E) implants at day 90 to promote carcinogenesis with aging. Epithelial stem and progenitor cells were isolated by prostasphere (PS) culture from dorsolateral prostates (DLP) of rats continuously exposed for six months to 2.5-250 mu g BPA/kg-BW. Gene expression was analyzed by quantitative real time reverse transcription polymerase chain reaction (qRT-PCR). RESULTS: Exposure to BPA alone at any dose did not drive prostate pathology. However, rats treated with EE, 2.5, 250, or 25,000 mu g BPA/kg-BW plus T+E showed greater severity of lateral prostate intraepithelial neoplasia (PIN), and DLP ductal adenocarcinoma multiplicity was markedly elevated in tumor-bearing rats exposed to 2.5 mu g BPA/kg-BW. DLP stem cells, assessed by PS number, doubled with chronic HE and 2.5 mu g BPA/kg-BW exposures. PS size, reflecting progenitor cell proliferation, was greater at 25 and 250 mu g BPA doses, which also shifted lineage commitment toward basal progenitors while reducing luminal progenitor cells. CONCLUSIONS: Together, these results confirm and extend previous evidence using a rat model and human prostate epithelial cells that low-dose BPA augments prostate cancer susceptibility and alters adult prostate stem cell homeostasis. Therefore, we propose that BPA exposures may contribute to the increased carcinogenic risk in humans that occurs with aging. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000452072200004 |
WOS关键词 | LIFE ENVIRONMENTAL EXPOSURES ; ANDROGEN RECEPTOR ; ESTROGEN ; GLAND ; CARCINOGENESIS ; TESTOSTERONE ; ESTRADIOL ; ALTERS ; DIFFERENTIATION ; IDENTIFICATION |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/23491 |
专题 | 资源环境科学 |
作者单位 | 1.UIC, Coll Med, Dept Urol, Chicago, IL USA; 2.Univ Illinois, Canc Ctr, Chicago, IL USA; 3.Univ Illinois, Chicago Ctr Hlth & Environm, Chicago, IL USA; 4.Univ Illinois, Coll Med, Dept Pathol, Chicago, IL USA |
推荐引用方式 GB/T 7714 | Prins, Gail S.,Hu, Wen-Yang,Xie, Lishi,et al. Evaluation of Bisphenol A (BPA) Exposures on Prostate Stem Cell Homeostasis and Prostate Cancer Risk in the NCTR-Sprague-Dawley Rat: An NIEHS/FDA CLARITY-BPA Consortium Study[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2018,126(11). |
APA | Prins, Gail S..,Hu, Wen-Yang.,Xie, Lishi.,Shi, Guang-Bin.,Hu, Dan-Ping.,...&Bosland, Maarten C..(2018).Evaluation of Bisphenol A (BPA) Exposures on Prostate Stem Cell Homeostasis and Prostate Cancer Risk in the NCTR-Sprague-Dawley Rat: An NIEHS/FDA CLARITY-BPA Consortium Study.ENVIRONMENTAL HEALTH PERSPECTIVES,126(11). |
MLA | Prins, Gail S.,et al."Evaluation of Bisphenol A (BPA) Exposures on Prostate Stem Cell Homeostasis and Prostate Cancer Risk in the NCTR-Sprague-Dawley Rat: An NIEHS/FDA CLARITY-BPA Consortium Study".ENVIRONMENTAL HEALTH PERSPECTIVES 126.11(2018). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论