Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP3441 |
Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood | |
Kochmanski, Joseph J.1; Marchlewicz, Elizabeth H.1; Cavalcante, Raymond G.2; Perera, Bambarendage P. U.1; Sartor, Maureen A.2,3; Dolinoy, Dana C.1,4 | |
2018-07-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES |
ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2018 |
卷号 | 126期号:7 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | BACKGROUND: Epigenetic machinery plays an important role in genomic imprinting, a developmental process that establishes parent-of-origin specific monoallelic gene expression. Although a number of studies have investigated the role of 5-methylcytosine in imprinting control, the contribution of 5-hydroxymethylcytosine (5-hmC) to this epigenetic phenomenon remains unclear. OBJECTIVES: Using matched mouse blood samples (from mice at 2, 4, and 10 months of age), our objective was to examine the effects of perinatal bisphenol A (BPA) exposure (50 mu g/kg diet) on longitudinal 5-hmC patterns at imprinted regions. We also aimed to test the hypothesis that 5-hmC would show defined patterns at imprinted genes that persist across the life course. METHODS: Genome-wide 5-hmC levels were measured using hydroxymethylated DNA immunoprecipitation sequencing (HMeDIP-seq). Modeling of differential hydroxymethylation by BPA exposure was performed using a pipeline of bioinformatics tools, including the csaw R package. RESULTS: Based on BPA exposure, we identified 5,950 differentially hydroxymethylated regions (DHMRs), including 12 DHMRs that were annotated to murine imprinted genes Gnus, Grb10, Magi], Ktf14, Pde10a, Snrpn, Airn, Cmath, Ppp1r9a, Kemp Pluletr2, and Pde4d. When visualized, these imprinted gene DHMRs showed clear, consistent patterns of differential 5-hmC by developmental BPA exposure that persisted throughout adulthood, CONCLUSIONS: These data show long-term establishment of 5-hmC marks at imprinted loci during development. Further, the effect of perinatal BPA exposure on 5-hmC at specific imprinted loci indicates that developmental exposure to environmental toxicants may alter long-term imprinted gene regulation via an epigenetic mechanism. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000440142600015 |
WOS关键词 | EMBRYONIC STEM-CELLS ; OXIDATIVE STRESS ; GENE-EXPRESSION ; BINDING-SITES ; GNAS CLUSTER ; ENVIRONMENTAL EPIGENOMICS ; CYTOSINE METHYLATION ; TRANSCRIPTION FACTOR ; GERM-CELLS ; IN-VITRO |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/24158 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Michigan, Sch Publ Hlth, Dept Environm Hlth Sci, Ann Arbor, MI 48109 USA; 2.Univ Michigan, Sch Med, Dept Computat Med & Bioinformat, Ann Arbor, MI USA; 3.Univ Michigan, Sch Publ Hlth, Dept Biostat, Ann Arbor, MI 48109 USA; 4.Univ Michigan, Sch Publ Hlth, Dept Nutr Sci, Ann Arbor, MI 48109 USA |
推荐引用方式 GB/T 7714 | Kochmanski, Joseph J.,Marchlewicz, Elizabeth H.,Cavalcante, Raymond G.,et al. Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2018,126(7). |
APA | Kochmanski, Joseph J.,Marchlewicz, Elizabeth H.,Cavalcante, Raymond G.,Perera, Bambarendage P. U.,Sartor, Maureen A.,&Dolinoy, Dana C..(2018).Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood.ENVIRONMENTAL HEALTH PERSPECTIVES,126(7). |
MLA | Kochmanski, Joseph J.,et al."Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood".ENVIRONMENTAL HEALTH PERSPECTIVES 126.7(2018). |
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