| DOI | 10.1038/s41586-020-2067-5
|
| DNA-loop extruding condensin complexes can traverse one another |
| Li, Xun1; Zhang, Fei2; He, Haiying1,4; Berry, Joseph J.3; Zhu, Kai2; Xu, Tao1
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| 2020-02-12
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| 发表期刊 | NATURE
 |
| ISSN | 0028-0836
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| EISSN | 1476-4687
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| 出版年 | 2020
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| 文章类型 | Article;Early Access
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| 语种 | 英语
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| 国家 | Netherlands; Germany |
| 英文关键词 | Condensin, a key component of the structure maintenance of chromosome (SMC) protein complexes, has recently been shown to be a motor that extrudes loops of DNA(1). It remains unclear, however, how condensin complexes work together to collectively package DNA into chromosomes. Here we use time-lapse single-molecule visualization to study mutual interactions between two DNA-loop-extruding yeast condensins. We find that these motor proteins, which, individually, extrude DNA in one direction only are able to dynamically change each other'
s DNA loop sizes, even when far apart. When they are in close proximity, condensin complexes are able to traverse each other and form a loop structure, which we term a Z-loop-three double-stranded DNA helices aligned in parallel with one condensin at each edge. Z-loops can fill gaps left by single loops and can form symmetric dimer motors that pull in DNA from both sides. These findings indicate that condensin may achieve chromosomal compaction using a variety of looping structures.
Single-molecule visualization shows that condensin-a motor protein that extrudes DNA in one direction only-can encounter and pass a second condensin molecule to form a new type of DNA loop that gathers DNA from both sides.
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| 领域 | 地球科学
; 气候变化
; 资源环境
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| 收录类别 | SCI-E
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| WOS记录号 | WOS:000518098200004
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| WOS关键词 | ORGANIZATION
; MODEL
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| WOS类目 | Multidisciplinary Sciences
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| WOS研究方向 | Science & Technology - Other Topics
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| 引用统计 |
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| 文献类型 | 期刊论文
|
| 条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/281282
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| 专题 | 地球科学
资源环境科学
气候变化
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| 作者单位 | 1.Northern Illinois Univ, Dept Chem & Biochem, De Kalb, IL 60115 USA;
2.Natl Renewable Energy Lab, Chem & Nanosci Ctr, Golden, CO 80401 USA;
3.Natl Renewable Energy Lab, Ctr Mat Sci, Golden, CO USA;
4.Valparaiso Univ, Dept Phys & Astron, Valparaiso, IN 46383 USA
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推荐引用方式
GB/T 7714 |
Li, Xun,Zhang, Fei,He, Haiying,et al. DNA-loop extruding condensin complexes can traverse one another[J].
NATURE,2020.
|
| APA |
Li, Xun,Zhang, Fei,He, Haiying,Berry, Joseph J.,Zhu, Kai,&Xu, Tao.(2020).DNA-loop extruding condensin complexes can traverse one another.NATURE.
|
| MLA |
Li, Xun,et al."DNA-loop extruding condensin complexes can traverse one another".NATURE (2020).
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