DNA-loop extruding condensin complexes can traverse one another

doi:10.1038/s41586-020-2067-5

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2067-5
	DNA-loop extruding condensin complexes can traverse one another
	Li, Xun 1; Zhang, Fei 2; He, Haiying 1,4; Berry, Joseph J.3; Zhu, Kai 2; Xu, Tao 1
	2020-02-12
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
文章类型	Article;Early Access
语种	英语
国家	Netherlands; Germany
英文关键词	Condensin, a key component of the structure maintenance of chromosome (SMC) protein complexes, has recently been shown to be a motor that extrudes loops of DNA(1). It remains unclear, however, how condensin complexes work together to collectively package DNA into chromosomes. Here we use time-lapse single-molecule visualization to study mutual interactions between two DNA-loop-extruding yeast condensins. We find that these motor proteins, which, individually, extrude DNA in one direction only are able to dynamically change each other' s DNA loop sizes, even when far apart. When they are in close proximity, condensin complexes are able to traverse each other and form a loop structure, which we term a Z-loop-three double-stranded DNA helices aligned in parallel with one condensin at each edge. Z-loops can fill gaps left by single loops and can form symmetric dimer motors that pull in DNA from both sides. These findings indicate that condensin may achieve chromosomal compaction using a variety of looping structures. Single-molecule visualization shows that condensin-a motor protein that extrudes DNA in one direction only-can encounter and pass a second condensin molecule to form a new type of DNA loop that gathers DNA from both sides.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000518098200004
WOS关键词	ORGANIZATION ; MODEL
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281282
专题	地球科学资源环境科学气候变化
作者单位	1.Northern Illinois Univ, Dept Chem & Biochem, De Kalb, IL 60115 USA; 2.Natl Renewable Energy Lab, Chem & Nanosci Ctr, Golden, CO 80401 USA; 3.Natl Renewable Energy Lab, Ctr Mat Sci, Golden, CO USA; 4.Valparaiso Univ, Dept Phys & Astron, Valparaiso, IN 46383 USA
推荐引用方式 GB/T 7714	Li, Xun,Zhang, Fei,He, Haiying,et al. DNA-loop extruding condensin complexes can traverse one another[J]. NATURE,2020.
APA	Li, Xun,Zhang, Fei,He, Haiying,Berry, Joseph J.,Zhu, Kai,&Xu, Tao.(2020).DNA-loop extruding condensin complexes can traverse one another.NATURE.
MLA	Li, Xun,et al."DNA-loop extruding condensin complexes can traverse one another".NATURE (2020).