Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants

doi:10.5194/acp-16-12347-2016

GSTDTAP > 地球科学

DOI	10.5194/acp-16-12347-2016
	Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants
	Pascal Renard, Isabelle Canet, Martine Sancelme, Nolwenn Wirgot, Laurent Deguillaume, and Anne-Marie Delort
	2016-09-29
发表期刊	Atmospheric Chemistry and Physics
出版年	2016
英文摘要	A total of 480 microorganisms collected from 39 clouds sampled at the Puy de Dôme station (alt. 1465 m; 45°46′19′′ N, 2°57′52′′ E; Massif Central, France) were isolated and identified. This unique collection was screened for biosurfactant (surfactants of microbial origin) production by measuring the surface tension (σ) of the crude extracts, comprising the supernatants of the pure cultures, using the pendant drop technique. The results showed that 41 % of the tested strains were active producers (σ < 55 mN m⁻¹), with 7 % being extremely active (σ < 30 mN m⁻¹). The most efficient biosurfactant producers (σ < 45 mN m⁻¹) belong to a few bacterial genera (Pseudomonas and Xanthomonas) from the Υ-Proteobacteria class (78 %) and a yeast genus (Udeniomyces) from the Basidiomycota phylum (11 %). Some Bacillus strains from the Firmicutes phylum were also active but represented a small fraction of the collected population. Strains from the Actinobacteria phylum in the collection examined in the present study showed moderate biosurfactant production (45<σ < 55 mN m⁻¹). Pseudomonas (Υ-Proteobacteria), the most frequently detected genus in clouds, with some species issued from the phyllosphere, was the dominant group for the production of biosurfactants. We observed some correlations between the chemical composition of cloud water and the presence of biosurfactant-producing microorganisms, suggesting the “biogeography” of this production. Moreover, the potential impact of the production of biosurfactants by cloud microorganisms on atmospheric processes is discussed.
领域	地球科学
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/287928
专题	地球科学
推荐引用方式 GB/T 7714	Pascal Renard, Isabelle Canet, Martine Sancelme, Nolwenn Wirgot, Laurent Deguillaume, and Anne-Marie Delort. Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants[J]. Atmospheric Chemistry and Physics,2016.
APA	Pascal Renard, Isabelle Canet, Martine Sancelme, Nolwenn Wirgot, Laurent Deguillaume, and Anne-Marie Delort.(2016).Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants.Atmospheric Chemistry and Physics.
MLA	Pascal Renard, Isabelle Canet, Martine Sancelme, Nolwenn Wirgot, Laurent Deguillaume, and Anne-Marie Delort."Screening of cloud microorganisms isolated at the Puy de Dôme (France) station for the production of biosurfactants".Atmospheric Chemistry and Physics (2016).