lncRNA <em>SLERT</em> controls phase separation of FC/DFCs to facilitate Pol I transcription

doi:10.1126/science.abf6582

GSTDTAP > 气候变化

DOI	10.1126/science.abf6582
	*lncRNA SLERT* controls phase separation of FC/DFCs to facilitate Pol I transcription**
	Man Wu; Guang Xu; Chong Han; Peng-Fei Luan; Yu-Hang Xing; Fang Nan; Liang-Zhong Yang; Youkui Huang; Zheng-Hu Yang; Lin Shan; Li Yang; Jiaquan Liu; Ling-Ling Chen
	2021-07-30
发表期刊	Science
出版年	2021
英文摘要	The nucleolus is a multilayered, membraneless nuclear condensate in which DNA polymerase I (Pol I)–mediated ribosomal DNA (rDNA) transcription and pre-rRNA processing occur in fibrillar center and dense fibrillar component (FC/DFC) units. How the biophysical properties of the nucleolus are regulated has remained elusive. Wu et al. found that the RNA helicase DDX21 forms a shell coating each FC/DFC unit in the nucleolus (see the Perspective by Yamazaki and Hirose). The authors found that a long noncoding RNA called SLERT facilitates the transition from the open to the closed configuration of the helicase using a chaperonelike mechanism. DDX21 in the closed conformation forms loose clusters that confer the FC/DFC unit sufficient liquidity and space required for Pol I processivity. In addition, DDX21 within the loose clusters cannot approach and wrap rDNA, thus licensing rDNA for transcription. Science , abf6582, this issue p. [547][1]; see also abj8350, p. [486][2] RNA polymerase I (Pol I) transcription takes place at the border of the fibrillar center (FC) and the dense fibrillar component (DFC) in the nucleolus. Here, we report that individual spherical FC/DFC units are coated by the DEAD-box RNA helicase DDX21 in human cells. The long noncoding RNA (lncRNA) SLERT binds to DDX21 RecA domains to promote DDX21 to adopt a closed conformation at a substoichiometric ratio through a molecular chaperone–like mechanism resulting in the formation of hypomultimerized and loose DDX21 clusters that coat DFCs, which is required for proper FC/DFC liquidity and Pol I processivity. Our results suggest that SLERT is an RNA regulator that controls the biophysical properties of FC/DFCs and thus ribosomal RNA production. [1]: /lookup/doi/10.1126/science.abf6582 [2]: /lookup/doi/10.1126/science.abj8350
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/335539
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Man Wu,Guang Xu,Chong Han,et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science,2021.
APA	Man Wu.,Guang Xu.,Chong Han.,Peng-Fei Luan.,Yu-Hang Xing.,...&Ling-Ling Chen.(2021).lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.Science.
MLA	Man Wu,et al."lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription".Science (2021).