Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes

doi:10.1126/science.abj1342

GSTDTAP > 气候变化

DOI	10.1126/science.abj1342
	Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes
	Federico Sabbadin; Saioa Urresti; Bernard Henrissat; Anna O. Avrova; Lydia R. J. Welsh; Peter J. Lindley; Michael Csukai; Julie N. Squires; Paul H. Walton; Gideon J. Davies; Neil C. Bruce; Stephen C. Whisson; Simon J. McQueen-Mason
	2021-08-13
发表期刊	Science
出版年	2021
英文摘要	P hytophthora infestans is a plant oomycete pathogen that drove the potato famines of the 1800s and continues to afflict potato fields today. The polysaccharide pectin makes up about a third of the cell wall in potatoes. Sabbadin et al. identified a family of lytic polysaccharide monooxygenases (LMPOs) that cleave pectin and are up-regulated in P. infestans during infection. Silencing the relevant LMPO gene successfully inhibited P. infestans infections. These findings open doors for disease intervention targets and for biotech applications. Science , abj1342, this issue p. [774][1] The oomycete Phytophthora infestans is a damaging crop pathogen and a model organism to study plant-pathogen interactions. We report the discovery of a family of copper-dependent lytic polysaccharide monooxygenases (LPMOs) in plant pathogenic oomycetes and its role in plant infection by P. infestans . We show that LPMO-encoding genes are up-regulated early during infection and that the secreted enzymes oxidatively cleave the backbone of pectin, a charged polysaccharide in the plant cell wall. The crystal structure of the most abundant of these LPMOs sheds light on its ability to recognize and degrade pectin, and silencing the encoding gene in P. infestans inhibits infection of potato, indicating a role in host penetration. The identification of LPMOs as virulence factors in pathogenic oomycetes opens up opportunities in crop protection and food security. [1]: /lookup/doi/10.1126/science.abj1342
领域	气候变化 ; 资源环境
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文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/335880
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Federico Sabbadin,Saioa Urresti,Bernard Henrissat,et al. Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes[J]. Science,2021.
APA	Federico Sabbadin.,Saioa Urresti.,Bernard Henrissat.,Anna O. Avrova.,Lydia R. J. Welsh.,...&Simon J. McQueen-Mason.(2021).Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes.Science.
MLA	Federico Sabbadin,et al."Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes".Science (2021).