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In this paper we validate the Lidar-Radiometer Inversion Code (URIC) retrievals of the aerosol concentration in the fine mode, using the airborne aerosol chemical composition dataset obtained over the Greater Athens Area (GAA) in Greece, during the ACEMED campaign. The study focuses on the 2nd of September 2011, when a long-range transported smoke layer was observed in the free troposphere over Greece, in the height range from 2 to 3 km. CIMEL sun-photometric measurements revealed high AOD (similar to 0.4 at 532 nm) and Angstrom exponent values (similar to 1.7 at 440/870 nm), in agreement with coincident ground-based lidar observations. Airborne chemical composition measurements performed over the GM, revealed increased CO volume concentration (similar to 110 ppbv), with 57% sulphate dominance in the PM1 fraction. For this case, we compare URIC retrievals of the aerosol concentration in the fine mode with the airborne Aerosol Mass Spectrometer (AMS) and Passive Cavity Aerosol Spectrometer Probe (PCASP) measurements. Our analysis shows that the remote sensing retrievals are in a good agreement with the measured airborne in-situ data from 2 to 4 km. The discrepancies observed between URIC and airborne measurements at the lower troposphere (below 2 km), could be explained by the spatial and temporal variability of the aerosol load within the area where the airborne data were averaged along with the different time windows of the retrievals. (C) 2016 Elsevier B.V. All rights reserved.