GSTDTAP  > 地球科学
DOI10.2172/985244
报告编号LBNL-3640E-Poster
来源IDOSTI ID: 985244
Revisiting Modes of energy generation in sulfate reducing bacteria
Joachimiak, Marcin; Chakraborty, Romy; Zhou, Aifen; Fortney, Julian; Geller, Jil; Wall, Judy; Zhou, Jizhong; Arkin, Adam; Hazen, Terry; Keasling, Jay; Chhabra, Swapnil
2010-05-17
出版年2010
语种英语
国家美国
领域地球科学
英文摘要Sulfate reducing bacteria (SRB) play an important role in global sulfur and carbon cycling through their ability to completely mineralize organic matter while respiring sulfate to hydrogen sulfide. They are ubiquitous in anaerobic environments and have the ability to reduce toxic metals like Cr(VI) and U(VI). While SRB have been studied for over three decades, bioenergetic modes of this group of microbes are poorly understood. Desulfovibrio vulgaris strain Hildenborough (DvH) has served as a model SRB over the last decade with the accumulation of transcriptomic, proteomic and metabolic data under a wide variety of stressors. To further investigate the three hypothesized modes of energy generation in this anaerobe we conducted a systematic study involving multiple electron donor and acceptor combinations for growth. DvH was grown at 37oC in a defined medium with (a) lactate + thiosulfate, (b) lactate + sulfite (c) lactate + sulfate, (d) pyruvate + sulfate, (e) H2 + acetate + sulfate, (f) formate + acetate + sulfate, g) formate + sulfate and (h) pyruvate fermentation. Cells were harvested at mid-log phase of growth for all conditions for transcriptomics, when the optical density at 600nm was in the range 0.42-0.5. Initial results indicate that cells grown on lactate do not appear to significantly differentiate their gene expression profiles when presented with different electron acceptors. These profiles however differ significantly from those observed during growth with other electron donors such as H2 and formate, as well as during fermentative growth. Together the gene expression changes in the presence of different electron donors provide insights into the ability of DvH to differentially reduce metals such as Cr(VI). Here we present revised modes of energy generation in DvH in light of this new transcriptomic evidence.
英文关键词Sulfate reducing bacteria mineralize organic matter Desulfovibrio vulgaris strain Hildenborough (DvH)
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来源平台US Department of Energy (DOE)
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文献类型科技报告
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/5049
专题地球科学
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Joachimiak, Marcin,Chakraborty, Romy,Zhou, Aifen,et al. Revisiting Modes of energy generation in sulfate reducing bacteria,2010.
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