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Field-resolved infrared spectroscopy of biological systems 期刊论文
NATURE, 2020, 577 (7788) : 52-+
作者:  Pupeza, Ioachim;  Huber, Marinus;  Trubetskov, Michael;  Schweinberger, Wolfgang;  Hussain, Syed A.;  Hofer, Christina;  Fritsch, Kilian;  Poetzlberger, Markus;  Vamos, Lenard;  Fill, Ernst;  Amotchkina, Tatiana;  Kepesidis, Kosmas V.;  Apolonski, Alexander;  Karpowicz, Nicholas;  Pervak, Vladimir;  Pronin, Oleg;  Fleischmann, Frank;  Azzeer, Abdallah;  Zigman, Mihaela;  Krausz, Ferenc
收藏  |  浏览/下载:14/0  |  提交时间:2020/07/03

The proper functioning of living systems and physiological phenotypes depends on molecular composition. Yet simultaneous quantitative detection of a wide variety of molecules remains a challenge(1-8). Here we show how broadband optical coherence opens up opportunities for fingerprinting complex molecular ensembles in their natural environment. Vibrationally excited molecules emit a coherent electric field following few-cycle infrared laser excitation(9-12), and this field is specific to the sample'  s molecular composition. Employing electro-optic sampling(10,12-15), we directly measure this global molecular fingerprint down to field strengths 10(7) times weaker than that of the excitation. This enables transillumination of intact living systems with thicknesses of the order of 0.1 millimetres, permitting broadband infrared spectroscopic probing of human cells and plant leaves. In a proof-of-concept analysis of human blood serum, temporal isolation of the infrared electric-field fingerprint from its excitation along with its sampling with attosecond timing precision results in detection sensitivity of submicrograms per millilitre of blood serum and a detectable dynamic range of molecular concentration exceeding 10(5). This technique promises improved molecular sensitivity and molecular coverage for probing complex, real-world biological and medical settings.


  
Observation of Bose-Einstein condensates in an Earth-orbiting research lab 期刊论文
NATURE, 2020, 582 (7811) : 103-+
作者:  Yamamoto, Keisuke;  Venida, Anthony;  Yano, Julian;  Biancur, Douglas E.;  Kakiuchi, Miwako;  Gupta, Suprit;  Sohn, Albert S. W.;  Mukhopadhyay, Subhadip;  Lin, Elaine Y.;  Parker, Seth J.;  Banh, Robert S.;  Paulo, Joao A.;  Wen, Kwun Wah;  Debnath, Jayanta;  Kim, Grace E.;  Mancias, Joseph D.;  Fearon, Douglas T.;  Perera, Rushika M.;  Kimmelman, Alec C.
收藏  |  浏览/下载:25/0  |  提交时间:2020/07/03

Quantum mechanics governs the microscopic world, where low mass and momentum reveal a natural wave-particle duality. Magnifying quantum behaviour to macroscopic scales is a major strength of the technique of cooling and trapping atomic gases, in which low momentum is engineered through extremely low temperatures. Advances in this field have achieved such precise control over atomic systems that gravity, often negligible when considering individual atoms, has emerged as a substantial obstacle. In particular, although weaker trapping fields would allow access to lower temperatures(1,2), gravity empties atom traps that are too weak. Additionally, inertial sensors based on cold atoms could reach better sensitivities if the free-fall time of the atoms after release from the trap could be made longer(3). Planetary orbit, specifically the condition of perpetual free-fall, offers to lift cold-atom studies beyond such terrestrial limitations. Here we report production of rubidium Bose-Einstein condensates (BECs) in an Earth-orbiting research laboratory, the Cold Atom Lab. We observe subnanokelvin BECs in weak trapping potentials with free-expansion times extending beyond one second, providing an initial demonstration of the advantages offered by a microgravity environment for cold-atom experiments and verifying the successful operation of this facility. With routine BEC production, continuing operations will support long-term investigations of trap topologies unique to microgravity(4,5), atom-laser sources(6), few-body physics(7,8)and pathfinding techniques for atom-wave interferometry(9-12).


  
An engineered PET depolymerase to break down and recycle plastic bottles 期刊论文
NATURE, 2020, 580 (7802) : 216-+
作者:  Zhao, Evan Wenbo;  Liu, Tao;  Jonsson, Erlendur;  Lee, Jeongjae;  Temprano, Israel;  Jethwa, Rajesh B.;  Wang, Anqi;  Smith, Holly;  Carretero-Gonzalez, Javier;  Song, Qilei;  Grey, Clare P.
收藏  |  浏览/下载:86/0  |  提交时间:2020/07/03

Present estimates suggest that of the 359 million tons of plastics produced annually worldwide(1), 150-200 million tons accumulate in landfill or in the natural environment(2). Poly(ethylene terephthalate) (PET) is the most abundant polyester plastic, with almost 70 million tons manufactured annually worldwide for use in textiles and packaging(3). The main recycling process for PET, via thermomechanical means, results in a loss of mechanical properties(4). Consequently, de novo synthesis is preferred and PET waste continues to accumulate. With a high ratio of aromatic terephthalate units-which reduce chain mobility-PET is a polyester that is extremely difficult to hydrolyse(5). Several PET hydrolase enzymes have been reported, but show limited productivity(6,7). Here we describe an improved PET hydrolase that ultimately achieves, over 10 hours, a minimum of 90 per cent PET depolymerization into monomers, with a productivity of 16.7 grams of terephthalate per litre per hour (200 grams per kilogram of PET suspension, with an enzyme concentration of 3 milligrams per gram of PET). This highly efficient, optimized enzyme outperforms all PET hydrolases reported so far, including an enzyme(8,9) from the bacterium Ideonella sakaiensis strain 201-F6 (even assisted by a secondary enzyme(10)) and related improved variants(11-14) that have attracted recent interest. We also show that biologically recycled PET exhibiting the same properties as petrochemical PET can be produced from enzymatically depolymerized PET waste, before being processed into bottles, thereby contributing towards the concept of a circular PET economy.


Computer-aided engineering produces improvements to an enzyme that breaks down poly(ethylene terephthalate) (PET) into its constituent monomers, which are used to synthesize PET of near-petrochemical grade that can be further processed into bottles.


  
Power generation from ambient humidity using protein nanowires 期刊论文
NATURE, 2020, 578 (7796) : 550-+
作者:  Luong, Duy X.;  Bets, Ksenia V.;  Algozeeb, Wala Ali;  Stanford, Michael G.;  Kittrell, Carter;  Chen, Weiyin;  Salvatierra, Rodrigo V.;  Ren, Muqing;  McHugh, Emily A.;  Advincula, Paul A.;  Wang, Zhe;  Bhatt, Mahesh;  Guo, Hua;  Mancevski, Vladimir;  Shahsavari, Rouzbeh;  Yakobson, Boris I.;  Tour, James M.
收藏  |  浏览/下载:85/0  |  提交时间:2020/07/03

Harvesting energy from the environment offers the promise of clean power for self-sustained systems(1,2). Known technologies-such as solar cells, thermoelectric devices and mechanical generators-have specific environmental requirements that restrict where they can be deployed and limit their potential for continuous energy production(3-5). The ubiquity of atmospheric moisture offers an alternative. However, existing moisture-based energy-harvesting technologies can produce only intermittent, brief (shorter than 50 seconds) bursts of power in the ambient environment, owing to the lack of a sustained conversion mechanism(6-12). Here we show that thin-film devices made from nanometre-scale protein wires harvested from the microbe Geobacter sulfurreducens can generate continuous electric power in the ambient environment. The devices produce a sustained voltage of around 0.5 volts across a 7-micrometre-thick film, with a current density of around 17 microamperes per square centimetre. We find the driving force behind this energy generation to be a self-maintained moisture gradient that forms within the film when the film is exposed to the humidity that is naturally present in air. Connecting several devices linearly scales up the voltage and current to power electronics. Our results demonstrate the feasibility of a continuous energy-harvesting strategy that is less restricted by location or environmental conditions than other sustainable approaches.


A new type of energy-harvesting device, based on protein nanowires from the microbe Geobacter sulforreducens, can generate a sustained power output by producing a moisture gradient across the nanowire film using natural humidity.


  
Transgenic Metarhizium rapidly kills mosquitoes in a malaria-endemic region of Burkina Faso 期刊论文
SCIENCE, 2019, 364 (6443) : 894-+
作者:  Lovett, Brian;  Bilgo, Etienne;  Millogo, Souro Abel;  Ouattarra, Abel Kader;  Sare, Issiaka;  Gnambani, Edounou Jacques;  Dabire, Roch K.;  Diabate, Abdoulaye;  Leger, Raymond J. St.
收藏  |  浏览/下载:5/0  |  提交时间:2019/11/27
Mechanoresponsive self-growing hydrogels inspired by muscle training 期刊论文
SCIENCE, 2019, 363 (6426) : 504-+
作者:  Matsuda, Takahiro;  Kawakami, Runa;  Namba, Ryo;  Nakajima, Tasuku;  Gong, Jian Ping
收藏  |  浏览/下载:4/0  |  提交时间:2019/11/27
Miami heat: Urban heat islands influence the thermal suitability of habitats for ectotherms 期刊论文
GLOBAL CHANGE BIOLOGY, 2019, 25 (2) : 562-576
作者:  Battles, Andrew C.;  Kolbe, Jason J.
收藏  |  浏览/下载:1/0  |  提交时间:2019/04/09
Anolis  canopy cover  operative temperature  thermal performance  thermal preference  thermoregulation  urbanization  
Residential green space in childhood is associated with lower risk of psychiatric disorders from adolescence into adulthood 期刊论文
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (11) : 5188-5193
作者:  Engemann, Kristine;  Pedersen, Carsten Bocker;  Arge, Lars;  Tsirogiannis, Constantinos;  Mortensen, Preben Bo;  Svenning, Jens-Christian
收藏  |  浏览/下载:9/0  |  提交时间:2019/11/27
geographic information systems  mental health  psychological ecosystem services  remote sensing  urban planning  
Natural forests exhibit higher carbon sequestration and lower water consumption than planted forests in China 期刊论文
GLOBAL CHANGE BIOLOGY, 2019, 25 (1) : 68-77
作者:  Yu, Zhen;  Liu, Shirong;  Wang, Jingxin;  Wei, Xiaohua;  Schuler, Jamie;  Sun, Pengsen;  Harper, Richard;  Zegre, Nicolas
收藏  |  浏览/下载:12/0  |  提交时间:2019/04/09
carbon sequestration  climate change  natural forests  planted forests  water consumption  water yield  
Contingency and determinism in evolution: Replaying life's tape 期刊论文
SCIENCE, 2018, 362 (6415)
作者:  Blount, Zachary D.;  Lenski, Richard E.;  Losos, Jonathan B.
收藏  |  浏览/下载:12/0  |  提交时间:2019/11/27