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Coupling delay controls synchronized oscillation in the segmentation clock 期刊论文
NATURE, 2020
作者:  Yoshioka-Kobayashi, Kumiko;  Matsumiya, Marina;  Niino, Yusuke;  Isomura, Akihiro;  Kori, Hiroshi;  Miyawaki, Atsushi;  Kageyama, Ryoichiro
收藏  |  浏览/下载:18/0  |  提交时间:2020/07/03

Individual cellular activities fluctuate but are constantly coordinated at the population level via cell-cell coupling. A notable example is the somite segmentation clock, in which the expression of clock genes (such as Hes7) oscillates in synchrony between the cells that comprise the presomitic mesoderm (PSM)(1,2). This synchronization depends on the Notch signalling pathway  inhibiting this pathway desynchronizes oscillations, leading to somite fusion(3-7). However, how Notch signalling regulates the synchronicity of HES7 oscillations is unknown. Here we establish a live-imaging system using a new fluorescent reporter (Achilles), which we fuse with HES7 to monitor synchronous oscillations in HES7 expression in the mouse PSM at a single-cell resolution. Wild-type cells can rapidly correct for phase fluctuations in HES7 oscillations, whereas the absence of the Notch modulator gene lunatic fringe (Lfng) leads to a loss of synchrony between PSM cells. Furthermore, HES7 oscillations are severely dampened in individual cells of Lfng-null PSM. However, when Lfng-null PSM cells were completely dissociated, the amplitude and periodicity of HES7 oscillations were almost normal, which suggests that LFNG is involved mostly in cell-cell coupling. Mixed cultures of control and Lfng-null PSM cells, and an optogenetic Notch signalling reporter assay, revealed that LFNG delays the signal-sending process of intercellular Notch signalling transmission. These results-together with mathematical modelling-raised the possibility that Lfng-null PSM cells shorten the coupling delay, thereby approaching a condition known as the oscillation or amplitude death of coupled oscillators(8). Indeed, a small compound that lengthens the coupling delay partially rescues the amplitude and synchrony of HES7 oscillations in Lfng-null PSM cells. Our study reveals a delay control mechanism of the oscillatory networks involved in somite segmentation, and indicates that intercellular coupling with the correct delay is essential for synchronized oscillation.


Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.


  
FLEXPART v10.1 simulation of source contributions to Arctic black carbon 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2020, 20 (3) : 1641-1656
作者:  Zhu, Chunmao;  Kanaya, Yugo;  Takigawa, Masayuki;  Ikeda, Kohei;  Tanimoto, Hiroshi;  Taketani, Fumikazu;  Miyakawa, Takuma;  Kobayashi, Hideki;  Pisso, Ignacio
收藏  |  浏览/下载:29/0  |  提交时间:2020/07/02
Fully hardware-implemented memristor convolutional neural network 期刊论文
NATURE, 2020, 577 (7792) : 641-+
作者:  Yoshioka-Kobayashi, Kumiko;  Matsumiya, Marina;  Niino, Yusuke;  Isomura, Akihiro;  Kori, Hiroshi;  Miyawaki, Atsushi;  Kageyama, Ryoichiro
收藏  |  浏览/下载:57/0  |  提交时间:2020/07/03

Memristor-enabled neuromorphic computing systems provide a fast and energy-efficient approach to training neural networks(1-4). However, convolutional neural networks (CNNs)-one of the most important models for image recognition(5)-have not yet been fully hardware-implemented using memristor crossbars, which are cross-point arrays with a memristor device at each intersection. Moreover, achieving software-comparable results is highly challenging owing to the poor yield, large variation and other non-ideal characteristics of devices(6-9). Here we report the fabrication of high-yield, high-performance and uniform memristor crossbar arrays for the implementation of CNNs, which integrate eight 2,048-cell memristor arrays to improve parallel-computing efficiency. In addition, we propose an effective hybrid-training method to adapt to device imperfections and improve the overall system performance. We built a five-layer memristor-based CNN to perform MNIST10 image recognition, and achieved a high accuracy of more than 96 per cent. In addition to parallel convolutions using different kernels with shared inputs, replication of multiple identical kernels in memristor arrays was demonstrated for processing different inputs in parallel. The memristor-based CNN neuromorphic system has an energy efficiency more than two orders of magnitude greater than that of state-of-the-art graphics-processing units, and is shown to be scalable to larger networks, such as residual neural networks. Our results are expected to enable a viable memristor-based non-von Neumann hardware solution for deep neural networks and edge computing.


  
A rice gene that confers broad-spectrum resistance to beta-triketone herbicides 期刊论文
SCIENCE, 2019, 365 (6451) : 393-396
作者:  Maeda, Hideo;  Murata, Kazumasa;  Sakuma, Nozomi;  Takei, Satomi;  Yamazaki, Akihiko;  Karim, Md. Rezaul;  Kawata, Motoshige;  Hirose, Sakiko;  Kawagishi-Kobayashi, Makiko;  Taniguchi, Yojiro;  Suzuki, Satoru;  Sekino, Keisuke;  Ohshima, Masahiro;  Kato, Hiroshi;  Yoshida, Hitoshi;  Tozawa, Yuzuru
收藏  |  浏览/下载:12/0  |  提交时间:2019/11/27
Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment 期刊论文
NATURE COMMUNICATIONS, 2018, 9
作者:  Ashraf, Shazia;  Kudo, Hiroki;  Rao, Jia;  Kikuchi, Atsuo;  Widmeier, Eugen;  Lawson, Jennifer A.;  Tan, Weizhen;  Hermle, Tobias;  Warejko, Jillian K.;  Shril, Shirlee;  Airik, Merlin;  Jobst-Schwan, Tilman;  Lovric, Svjetlana;  Braun, Daniela A.;  Gee, Heon Yung;  Schapiro, David;  Majmundar, Amar J.;  Sadowski, Carolin E.;  Pabst, Werner L.;  Daga, Ankana;  van der Ven, Amelie T.;  Schmidt, Johanna M.;  Low, Boon Chuan;  Gupta, Anjali Bansal;  Tripathi, Brajendra K.;  Wong, Jenny;  Campbell, Kirk;  Metcalfe, Kay;  Schanze, Denny;  Niihori, Tetsuya;  Kaito, Hiroshi;  Nozu, Kandai;  Tsukaguchi, Hiroyasu;  Tanaka, Ryojiro;  Hamahira, Kiyoshi;  Kobayashi, Yasuko;  Takizawa, Takumi;  Funayama, Ryo;  Nakayama, Keiko;  Aoki, Yoko;  Kumagai, Naonori;  Iijima, Kazumoto;  Fehrenbach, Henry;  Kari, Jameela A.;  El Desoky, Sherif;  Jalalah, Sawsan;  Bogdanovic, Radovan;  Stajic, Natasa;  Zappel, Hildegard;  Rakhmetova, Assel;  Wassmer, Sharon-Rose;  Jungraithmayr, Therese;  Strehlau, Juergen;  Kumar, Aravind Selvin;  Bagga, Arvind;  Soliman, Neveen A.;  Mane, Shrikant M.;  Kaufman, Lewis;  Lowy, Douglas R.;  Jairajpuri, Mohamad A.;  Lifton, Richard P.;  Pei, York;  Zenker, Martin;  Kure, Shigeo;  Hildebrandt, Friedhelm
收藏  |  浏览/下载:32/0  |  提交时间:2019/11/27
Synergistic effect of water-soluble species and relative humidity on morphological changes in aerosol particles in the Beijing megacity during severe pollution episodes 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2019, 19 (1) : 219-232
作者:  Pan, Xiaole;  Ge, Baozhu;  Wang, Zhe;  Tian, Yu;  Liu, Hang;  Wei, Lianfang;  Yue, Siyao;  Uno, Itsushi;  Kobayashi, Hiroshi;  Nishizawa, Tomoaki;  Shimizu, Atsushi;  Fu, Pingqing;  Wang, Zifa
收藏  |  浏览/下载:16/0  |  提交时间:2019/04/09
Variability of depolarization of aerosol particles in the megacity of Beijing: implications for the interaction between anthropogenic pollutants and mineral dust particles 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2018, 18 (24) : 18203-18217
作者:  Tian, Yu;  Pan, Xiaole;  Nishizawa, Tomoaki;  Kobayashi, Hiroshi;  Uno, Itsushi;  Wang, Xiquan;  Shimizu, Atsushi;  Wang, Zifa
收藏  |  浏览/下载:19/0  |  提交时间:2019/04/09
Variations in airborne bacterial communities at high altitudes over the Noto Peninsula (Japan) in response to Asian dust events 期刊论文
ATMOSPHERIC CHEMISTRY AND PHYSICS, 2017, 17 (19)
作者:  Maki, Teruya;  Hara, Kazutaka;  Iwata, Ayumu;  Lee, Kevin C.;  Kawai, Kei;  Kai, Kenji;  Kobayashi, Fumihisa;  Pointing, Stephen B.;  Archer, Stephen;  Hasegawa, Hiroshi;  Iwasaka, Yasunobu
收藏  |  浏览/下载:15/0  |  提交时间:2019/04/09
Viscoelastic lower crust and mantle relaxation following the 14-16 April 2016 Kumamoto, Japan, earthquake sequence 期刊论文
GEOPHYSICAL RESEARCH LETTERS, 2017, 44 (17)
作者:  Pollitz, Fred F.;  Kobayashi, Tomokazu;  Yarai, Hiroshi;  Shibazaki, Bunichiro;  Matsumoto, Takumi
收藏  |  浏览/下载:6/0  |  提交时间:2019/04/09
Holliday junction resolvases mediate chloroplast nucleoid segregation 期刊论文
SCIENCE, 2017, 356 (6338) : 631-634
作者:  Kobayashi, Yusuke;  Misumi, Osami;  Odahara, Masaki;  Ishibashi, Kota;  Hirono, Masafumi;  Hidaka, Kumi;  Endo, Masayuki;  Sugiyama, Hiroshi;  Iwasaki, Hiroshi;  Kuroiwa, Tsuneyoshi;  Shikanai, Toshiharu;  Nishimura, Yoshiki
收藏  |  浏览/下载:14/0  |  提交时间:2019/11/27