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Cortical pattern generation during dexterous movement is input-driven 期刊论文
NATURE, 2020, 577 (7790) : 386-+
作者:  Cyranoski, David
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/03

The motor cortex controls skilled arm movement by sending temporal patterns of activity to lower motor centres(1). Local cortical dynamics are thought to shape these patterns throughout movement execution(2-4). External inputs have been implicated in setting the initial state of the motor cortex(5,6), but they may also have a pattern-generating role. Here we dissect the contribution of local dynamics and inputs to cortical pattern generation during a prehension task in mice. Perturbing cortex to an aberrant state prevented movement initiation, but after the perturbation was released, cortex either bypassed the normal initial state and immediately generated the pattern that controls reaching or failed to generate this pattern. The difference in these two outcomes was probably a result of external inputs. We directly investigated the role of inputs by inactivating the thalamus  this perturbed cortical activity and disrupted limb kinematics at any stage of the movement. Activation of thalamocortical axon terminals at different frequencies disrupted cortical activity and arm movement in a graded manner. Simultaneous recordings revealed that both thalamic activity and the current state of cortex predicted changes in cortical activity. Thus, the pattern generator for dexterous arm movement is distributed across multiple, strongly interacting brain regions.


  
The local effects of an innovation: Evidence from the French fish market 期刊论文
ECOLOGICAL ECONOMICS, 2020, 171
作者:  Gobillon, Laurent;  Wolff, Francois-Charles
收藏  |  浏览/下载:5/0  |  提交时间:2020/07/02
Fish  Innovation  Product quality  Product prices  Discontinuity  Difference in differences  
IL-17a promotes sociability in mouse models of neurodevelopmental disorders 期刊论文
NATURE, 2020, 577 (7789) : 249-+
作者:  Reed, Michael Douglas;  Yim, Yeong Shin;  Wimmer, Ralf D.;  Kim, Hyunju;  Ryu, Changhyeon;  Welch, Gwyneth Margaret;  Andina, Matias;  King, Hunter Oren;  Waisman, Ari;  Halassa, Michael M.;  Huh, Jun R.;  Choi, Gloria B.
收藏  |  浏览/下载:13/0  |  提交时间:2020/07/03

A subset of children with autism spectrum disorder appear to show an improvement in their behavioural symptoms during the course of a fever, a sign of systemic inflammation(1,2). Here we elucidate the molecular and neural mechanisms that underlie the beneficial effects of inflammation on social behaviour deficits in mice. We compared an environmental model of neurodevelopmental disorders in which mice were exposed to maternal immune activation (MIA) during embryogenesis(3,4) with mouse models that are genetically deficient for contactin-associated protein-like 2 (Cntnap2)(5), fragile X mental retardation-1 (Fmr1)(6) or Sh3 and multiple ankyrin repeat domains 3 (Shank3)(7). We establish that the social behaviour deficits in offspring exposed to MIA can be temporarily rescued by the inflammatory response elicited by the administration of lipopolysaccharide (LPS). This behavioural rescue was accompanied by a reduction in neuronal activity in the primary somatosensory cortex dysgranular zone (S1DZ), the hyperactivity of which was previously implicated in the manifestation of behavioural phenotypes associated with offspring exposed to MIA(8). By contrast, we did not observe an LPS-induced rescue of social deficits in the monogenic models. We demonstrate that the differences in responsiveness to the LPS treatment between the MIA and the monogenic models emerge from differences in the levels of cytokine production. LPS treatment in monogenic mutant mice did not induce amounts of interleukin-17a (IL-17a) comparable to those induced in MIA offspring  bypassing this difference by directly delivering IL-17a into S1DZ was sufficient to promote sociability in monogenic mutant mice as well as in MIA offspring. Conversely, abrogating the expression of IL-17 receptor subunit a (IL-17Ra) in the neurons of the S1DZ eliminated the ability of LPS to reverse the sociability phenotypes in MIA offspring. Our data support a neuroimmune mechanism that underlies neurodevelopmental disorders in which the production of IL-17a during inflammation can ameliorate the expression of social behaviour deficits by directly affecting neuronal activity in the central nervous system.


  
Potential circadian effects on translational failure for neuroprotection 期刊论文
NATURE, 2020
作者:  Sakai, Akito;  Minami, Susumu;  Koretsune, Takashi;  Chen, Taishi;  Higo, Tomoya;  Wang, Yangming;  Nomoto, Takuya;  Hirayama, Motoaki;  Miwa, Shinji;  Nishio-Hamane, Daisuke;  Ishii, Fumiyuki;  Arita, Ryotaro;  Nakatsuji, Satoru
收藏  |  浏览/下载:15/0  |  提交时间:2020/07/03

Neuroprotectant strategies that have worked in rodent models of stroke have failed to provide protection in clinical trials. Here we show that the opposite circadian cycles in nocturnal rodents versus diurnal humans(1,2) may contribute to this failure in translation. We tested three independent neuroprotective approaches-normobaric hyperoxia, the free radical scavenger alpha-phenyl-butyl-tert-nitrone (alpha PBN), and the N-methyl-d-aspartic acid (NMDA) antagonist MK801-in mouse and rat models of focal cerebral ischaemia. All three treatments reduced infarction in day-time (inactive phase) rodent models of stroke, but not in night-time (active phase) rodent models of stroke, which match the phase (active, day-time) during which most strokes occur in clinical trials. Laser-speckle imaging showed that the penumbra of cerebral ischaemia was narrower in the active-phase mouse model than in the inactive-phase model. The smaller penumbra was associated with a lower density of terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive dying cells and reduced infarct growth from 12 to 72 h. When we induced circadian-like cycles in primary mouse neurons, deprivation of oxygen and glucose triggered a smaller release of glutamate and reactive oxygen species, as well as lower activation of apoptotic and necroptotic mediators, in '  active-phase'  than in '  inactive-phase'  rodent neurons. alpha PBN and MK801 reduced neuronal death only in '  inactive-phase'  neurons. These findings suggest that the influence of circadian rhythm on neuroprotection must be considered for translational studies in stroke and central nervous system diseases.


Studies in rats and mice at different times of day suggest that the failure of neuroprotective strategies for stroke in translational studies might be related to the difference in circadian cycles between humans and rodents.


  
Hydrothermal (NN)-N-15-N-15 abundances constrain the origins of mantle nitrogen 期刊论文
NATURE, 2020, 580 (7803) : 367-+
作者:  Zhao, Steven;  Jang, Cholsoon;  Liu, Joyce;  Uehara, Kahealani;  Gilbert, Michael;  Izzo, Luke;  Zeng, Xianfeng;  Trefely, Sophie;  Fernandez, Sully;  Carrer, Alessandro;  Miller, Katelyn D.;  Schug, Zachary T.;  Snyder, Nathaniel W.;  Gade, Terence P.;  Titchenell, Paul M.;  Rabinowitz, Joshua D.;  Wellen, Kathryn E.
收藏  |  浏览/下载:16/0  |  提交时间:2020/05/13

Nitrogen is the main constituent of the Earth'  s atmosphere, but its provenance in the Earth'  s mantle remains uncertain. The relative contribution of primordial nitrogen inherited during the Earth'  s accretion versus that subducted from the Earth'  s surface is unclear(1-6). Here we show that the mantle may have retained remnants of such primordial nitrogen. We use the rare (NN)-N-15-N-15 isotopologue of N-2 as a new tracer of air contamination in volcanic gas effusions. By constraining air contamination in gases from Iceland, Eifel (Germany) and Yellowstone (USA), we derive estimates of mantle delta N-15 (the fractional difference in N-15/N-14 from air), N-2/Ar-36 and N-2/He-3. Our results show that negative delta N-15 values observed in gases, previously regarded as indicating a mantle origin for nitrogen(7-10), in fact represent dominantly air-derived N-2 that experienced N-15/N-14 fractionation in hydrothermal systems. Using two-component mixing models to correct for this effect, the (NN)-N-15-N-15 data allow extrapolations that characterize mantle endmember delta N-15, N-2/Ar-36 and N-2/He-3 values. We show that the Eifel region has slightly increased delta N-15 and N-2/Ar-36 values relative to estimates for the convective mantle provided by mid-ocean-ridge basalts(11), consistent with subducted nitrogen being added to the mantle source. In contrast, we find that whereas the Yellowstone plume has delta N-15 values substantially greater than that of the convective mantle, resembling surface components(12-15), its N-2/Ar-36 and N-2/He-3 ratios are indistinguishable from those of the convective mantle. This observation raises the possibility that the plume hosts a primordial component. We provide a test of the subduction hypothesis with a two-box model, describing the evolution of mantle and surface nitrogen through geological time. We show that the effect of subduction on the deep nitrogen cycle may be less important than has been suggested by previous investigations. We propose instead that high mid-ocean-ridge basalt and plume delta N-15 values may both be dominantly primordial features.


  
Detection of metastable electronic states by Penning trap mass spectrometry 期刊论文
NATURE, 2020, 581 (7806) : 42-+
作者:  Rauch, Jennifer N.;  Luna, Gabriel;  Guzman, Elmer;  Audouard, Morgane;  Challis, Collin;  Sibih, Youssef E.;  Leshuk, Carolina;  Hernandez, Israel;  Wegmann, Susanne;  Hyman, Bradley T.;  Gradinaru, Viviana;  Kampmann, Martin;  Kosik, Kenneth S.
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/03

State-of-the-art optical clocks(1) achieve precisions of 10(-18) or better using ensembles of atoms in optical lattices(2,3) or individual ions in radio-frequency traps(4,5). Promising candidates for use in atomic clocks are highly charged ions(6) (HCIs) and nuclear transitions(7), which are largely insensitive to external perturbations and reach wavelengths beyond the optical range(8) that are accessible to frequency combs(9). However, insufficiently accurate atomic structure calculations hinder the identification of suitable transitions in HCIs. Here we report the observation of a long-lived metastable electronic state in an HCI by measuring the mass difference between the ground and excited states in rhenium, providing a non-destructive, direct determination of an electronic excitation energy. The result is in agreement with advanced calculations. We use the high-precision Penning trap mass spectrometer PENTATRAP to measure the cyclotron frequency ratio of the ground state to the metastable state of the ion with a precision of 10(-11)-an improvement by a factor of ten compared with previous measurements(10,11). With a lifetime of about 130 days, the potential soft-X-ray frequency reference at 4.96 x 10(16) hertz (corresponding to a transition energy of 202 electronvolts) has a linewidth of only 5 x 10(-8) hertz and one of the highest electronic quality factors (10(24)) measured experimentally so far. The low uncertainty of our method will enable searches for further soft-X-ray clock transitions(8,12) in HCIs, which are required for precision studies of fundamental physics(6).


Penning trap mass spectrometry is used to measure the electronic transition energy from a long-lived metastable state to the ground state in highly charged rhenium ions with a precision of 10(-11).


  
Using Natural Experiments and Counterfactuals for Causal Assessment: River Salinity and the Ganges Water Agreement 期刊论文
WATER RESOURCES RESEARCH, 2020, 56 (4)
作者:  Penny, Gopal;  Mondal, M. Shahjahan;  Biswas, Subir;  Bolster, Diogo;  Tank, Jennifer L.;  Mueller, Marc F.
收藏  |  浏览/下载:10/0  |  提交时间:2020/07/02
causal inference  transboundary treaty  difference in difference  India  Bangladesh  delta  
Simulation of Hubbard model physics in WSe2/WS2 moire superlattices 期刊论文
NATURE, 2020, 579 (7799) : 353-+
作者:  Stein, Reed M.;  Kang, Hye Jin;  McCorvy, John D.;  Glatfelter, Grant C.;  Jones, Anthony J.;  Che, Tao;  Slocum, Samuel;  Huang, Xi-Ping;  Savych, Olena;  Moroz, Yurii S.;  Stauch, Benjamin;  Johansson, Linda C.;  Cherezov, Vadim;  Kenakin, Terry;  Irwin, John J.;  Shoichet, Brian K.;  Roth, Bryan L.;  Dubocovich, Margarita L.
收藏  |  浏览/下载:9/0  |  提交时间:2020/07/03

Study of WSe2/WS2 moire superlattices reveals the phase diagram of the triangular-lattice Hubbard model, including a Mott insulating state at half-filling and a possible magnetic quantum phase transition near 0.6 filling.


The Hubbard model, formulated by physicist John Hubbard in the 1960s(1), is a simple theoretical model of interacting quantum particles in a lattice. The model is thought to capture the essential physics of high-temperature superconductors, magnetic insulators and other complex quantum many-body ground states(2,3). Although the Hubbard model provides a greatly simplified representation of most real materials, it is nevertheless difficult to solve accurately except in the one-dimensional case(2,3). Therefore, the physical realization of the Hubbard model in two or three dimensions, which can act as an analogue quantum simulator (that is, it can mimic the model and simulate its phase diagram and dynamics(4,5)), has a vital role in solving the strong-correlation puzzle, namely, revealing the physics of a large number of strongly interacting quantum particles. Here we obtain the phase diagram of the two-dimensional triangular-lattice Hubbard model by studying angle-aligned WSe2/WS2 bilayers, which form moire superlattices(6) because of the difference between the lattice constants of the two materials. We probe the charge and magnetic properties of the system by measuring the dependence of its optical response on an out-of-plane magnetic field and on the gate-tuned carrier density. At half-filling of the first hole moire superlattice band, we observe a Mott insulating state with antiferromagnetic Curie-Weiss behaviour, as expected for a Hubbard model in the strong-interaction regime(2,3,7-9). Above half-filling, our experiment suggests a possible quantum phase transition from an antiferromagnetic to a weak ferromagnetic state at filling factors near 0.6. Our results establish a new solid-state platform based on moire superlattices that can be used to simulate problems in strong-correlation physics that are described by triangular-lattice Hubbard models.


  
Asynchronous carbon sink saturation in African and Amazonian tropical forests 期刊论文
NATURE, 2020, 579 (7797) : 80-+
作者:  Wannes Hubau;  Simon L. Lewis;  Oliver L. Phillips;  Kofi Affum-Baffoe;  Hans Beeckman;  Aida Cuní;  -Sanchez;  Armandu K. Daniels;  Corneille E. N. Ewango;  Sophie Fauset;  Jacques M. Mukinzi;  Douglas Sheil;  Bonaventure Sonké;  Martin J. P. Sullivan;  Terry C. H. Sunderland;  Hermann Taedoumg;  Sean C. Thomas;  Lee J. T. White;  Katharine A. Abernethy;  Stephen Adu-Bredu;  Christian A. Amani;  Timothy R. Baker;  Lindsay F. Banin;  Fidè;  le Baya;  Serge K. Begne;  Amy C. Bennett;  Fabrice Benedet;  Robert Bitariho;  Yannick E. Bocko;  Pascal Boeckx;  Patrick Boundja;  Roel J. W. Brienen;  Terry Brncic;  Eric Chezeaux;  George B. Chuyong;  Connie J. Clark;  Murray Collins;  James A. Comiskey;  David A. Coomes;  Greta C. Dargie;  Thales de Haulleville;  Marie Noel Djuikouo Kamdem;  Jean-Louis Doucet;  Adriane Esquivel-Muelbert;  Ted R. Feldpausch;  Alusine Fofanah;  Ernest G. Foli;  Martin Gilpin;  Emanuel Gloor;  Christelle Gonmadje;  Sylvie Gourlet-Fleury;  Jefferson S. Hall;  Alan C. Hamilton;  David J. Harris;  Terese B. Hart;  Mireille B. N. Hockemba;  Annette Hladik;  Suspense A. Ifo;  Kathryn J. Jeffery;  Tommaso Jucker;  Emmanuel Kasongo Yakusu;  Elizabeth Kearsley;  David Kenfack;  Alexander Koch;  Miguel E. Leal;  Aurora Levesley;  Jeremy A. Lindsell;  Janvier Lisingo;  Gabriela Lopez-Gonzalez;  Jon C. Lovett;  Jean-Remy Makana;  Yadvinder Malhi;  Andrew R. Marshall;  Jim Martin;  Emanuel H. Martin;  Faustin M. Mbayu;  Vincent P. Medjibe;  Vianet Mihindou;  Edward T. A. Mitchard;  Sam Moore;  Pantaleo K. T. Munishi;  Natacha Nssi Bengone;  Lucas Ojo;  Fidè;  le Evouna Ondo;  Kelvin S.-H. Peh;  Georgia C. Pickavance;  Axel Dalberg Poulsen;  John R. Poulsen;  Lan Qie;  Jan Reitsma;  Francesco Rovero;  Michael D. Swaine;  Joey Talbot;  James Taplin;  David M. Taylor;  Duncan W. Thomas;  Benjamin Toirambe;  John Tshibamba Mukendi;  Darlington Tuagben;  Peter M. Umunay;  Geertje M. F. van der Heijden;  Hans Verbeeck;  Jason Vleminckx;  Simon Willcock;  Hannsjö;  rg Wö;  ll;  John T. Woods;  Lise Zemagho
收藏  |  浏览/下载:30/0  |  提交时间:2020/05/13

Structurally intact tropical forests sequestered about half of the global terrestrial carbon uptake over the 1990s and early 2000s, removing about 15 per cent of anthropogenic carbon dioxide emissions(1-3). Climate-driven vegetation models typically predict that this tropical forest '  carbon sink'  will continue for decades(4,5). Here we assess trends in the carbon sink using 244 structurally intact African tropical forests spanning 11 countries, compare them with 321 published plots from Amazonia and investigate the underlying drivers of the trends. The carbon sink in live aboveground biomass in intact African tropical forests has been stable for the three decades to 2015, at 0.66 tonnes of carbon per hectare per year (95 per cent confidence interval 0.53-0.79), in contrast to the long-term decline in Amazonian forests(6). Therefore the carbon sink responses of Earth'  s two largest expanses of tropical forest have diverged. The difference is largely driven by carbon losses from tree mortality, with no detectable multi-decadal trend in Africa and a long-term increase in Amazonia. Both continents show increasing tree growth, consistent with the expected net effect of rising atmospheric carbon dioxide and air temperature(7-9). Despite the past stability of the African carbon sink, our most intensively monitored plots suggest a post-2010 increase in carbon losses, delayed compared to Amazonia, indicating asynchronous carbon sink saturation on the two continents. A statistical model including carbon dioxide, temperature, drought and forest dynamics accounts for the observed trends and indicates a long-term future decline in the African sink, whereas the Amazonian sink continues to weaken rapidly. Overall, the uptake of carbon into Earth'  s intact tropical forests peaked in the 1990s. Given that the global terrestrial carbon sink is increasing in size, independent observations indicating greater recent carbon uptake into the Northern Hemisphere landmass(10) reinforce our conclusion that the intact tropical forest carbon sink has already peaked. This saturation and ongoing decline of the tropical forest carbon sink has consequences for policies intended to stabilize Earth'  s climate.


  
Long-term cyclic persistence in an experimental predator-prey system 期刊论文
NATURE, 2020, 577 (7789) : 226-+
作者:  Blasius, Bernd;  Rudolf, Lars;  Weithoff, Guntram;  Gaedke, Ursula;  Fussmann, Gregor F.
收藏  |  浏览/下载:11/0  |  提交时间:2020/04/16

Predator-prey cycles rank among the most fundamental concepts in ecology, are predicted by the simplest ecological models and enable, theoretically, the indefinite persistence of predator and prey(1-4). However, it remains an open question for how long cyclic dynamics can be self-sustained in real communities. Field observations have been restricted to a few cycle periods(5-8) and experimental studies indicate that oscillations may be short-lived without external stabilizing factors(9-19). Here we performed microcosm experiments with a planktonic predator-prey system and repeatedly observed oscillatory time series of unprecedented length that persisted for up to around 50 cycles or approximately 300 predator generations. The dominant type of dynamics was characterized by regular, coherent oscillations with a nearly constant predator-prey phase difference. Despite constant experimental conditions, we also observed shorter episodes of irregular, non-coherent oscillations without any significant phase relationship. However, the predator-prey system showed a strong tendency to return to the dominant dynamical regime with a defined phase relationship. A mathematical model suggests that stochasticity is probably responsible for the reversible shift from coherent to non-coherent oscillations, a notion that was supported by experiments with external forcing by pulsed nutrient supply. Our findings empirically demonstrate the potential for infinite persistence of predator and prey populations in a cyclic dynamic regime that shows resilience in the presence of stochastic events.